Study the effect of some plants extracts on growth of Mycobacterium tuberculosis in Comparison with Ethambutol

Abstract

This study was conducted to investigate the inhibition role of alcoholic
extracts of plants Trigonella foenumgraecum , Piper cubeba , Piper nigrum,
Camphora , Commiphora molmol, Apium graveolens , Arachis hypogaea ,
Linum usitatissumun , Cuminum cyminum , Pimpinella anisum , Lepidium
sativum , alcoholic extracts of plants used was prepared and used of
concentration 200 mg/ml and add 0.2 ml of each extracts to 10 ml of media and
0.2 ml of Ethambutol to 10 ml of media the drug for mycobacteria and media
with out any adding for controls. Cultural media were inoculated with 3 drops
of bacterial suspension ( 1mg /ml ) in concentration 108 cfu/ml from patient
suffering from tuberculosis and after incubation on 37 °C the result read
weekly up to 4 weeks . the results showed good growth on the control slopes ,
which was evident within 2-3 weeks and no growth on the cultural media
contain (Arachis hypogea , Cuminum cyminum , Commiphora molmol,
Trigonella foenumgraecum , Lepidium sativum , Ethambutol ) it while the
media contain (Piper nigrum , Pimpinella anisum showed than 5 colonies the
growth inhibition was recorded that means sensitivity of mycobacteria to the
extracts mention above, while media contain (Piper cubeba , Camphora , Apium
graveolens ). showed growth more than 5 colonies and media contain Linum
usitatissumun showed good growth which means resistant of mycobacteria to
the extract mentioned above .