Pathological and histochemical changes in the lungs of rabbits experimentally infected with P.seudomonas aeruginosa

Main Article Content

AL. Kutbi, S. H.
M. J. Alwan


The main objective to this study is to demonstrate certain aspects of pathological and histochomical changes in the lungs of rabbits experimentaly infected with P.aeruginosa

Twelve white Newzoland rabbits of 12-16 weeks old were used . they were randomaly divided into two groups. The first group (6 animals ) were given single intraperitoneal, dose of 108 CFU of P. aeruginosa . The second group (6 animals ) maintained as a control and was inoculated with 1 ml of sterial normal saline by the same route of the first group. All the animals were sacrificed on day three post – inoculation. 

The pathological exmination showed acute suppurative reaction in pulmonary tissue together with lymphocytic aggregation around airways and blood vessels. The histochomical study revealed that the granules of goblet cells and submucesal glands of the lung in normal rabbits were of neutral mucopolysaecharides and little sialomucin, these substances changed to sulfated acid mucopolysaccharide and sialomucin in the infected animals.


Download data is not yet available.

Article Details

How to Cite
H., A. K. S., & Alwan, M. J. (2001). Pathological and histochemical changes in the lungs of rabbits experimentally infected with P.seudomonas aeruginosa. The Iraqi Journal of Veterinary Medicine, 25(1), 24–39.


1- Vasil,M.L. (1986). Pseudomonas aeruginosa; Biology
mechanism of virulence, epidemiology. J.Podiat. 108 : 800 - 805.
2-Noas, T.I. and Glewski, B.H.(1985). the contribution of exoproducts to virulence of P.aeruginosa . Can. J. Microb. 31:387-392.
3-Liu, P.V.(1974). Extracellular toxins of P. aeruginosa J.Infect. Dis. 130:594-599.
4-Richards, M.J.; Edwards, J.R.; Culure, D.H. and Gaynes, R.P.(1999) . Nosocemial infection in pediatric intensive care units in the United State, Pediatrics. 103:39-40 .
5-Alwan, M.J. (1997). Histopathological and histochomical changes in the pulmonary tissues of cattle infected with hydatid cyst. Iraqi Vet. Med.J.21:80-92 .
6-Mory, R.W.(1956). Alciablue techniquos for the histochomical study of acidic carbehydrates .J. Histochem. 4:407-414 .
7-Spicer, S.S. (1960) . The use of various catinunic reagent in histochemical of mucapolysacchrides. Am.J. Clin. Path. 36:393-400.
8-Spicer, S.S. ; Horn, R.G. and Loppi, T.J.(1967). Histochomistry of connective tissue mucepolysacchrides. In the C.T. intornational academy of pathology, monograph No. 7. Williams Co. Baltimoro, PP. 251-303.
9-Spicer, S.S. and Moyer, D.B. (11960). Technical section . histochomical diffeenatiatien acid Mucepolysacchrides. by means of combined aldehyed fuchsin-alcian blue staining .
Am.J. Clin.Path. 33:435-460 .
10-Quinn, P.J.; Carter, M.E.; Markey, B.K. and Carter, G.R. (1994). In : Clinical Vet. Microbiol. Mosby , London . Philadolphia. St. louts. Sydney. Tokyo .
11-Reed, D.J. and Muench, H.(1938) Asimple of estimating fifty percent endpoints . Am. J.Hyg. 27: 493-497 .
12-Miles, A.A.; Misra, S.S. and Irwin, J.O. (1938) . The estimation of bactericidal power of blood .J. Hyg. 38:732-749.
13-Konig , B.; Vasil, M.L. and Koing, W. (1997). Role of hemolytic and nonhemolytic phosholipasec from P. aeruginosa for inflammatrory modiater release from human granulecytes. Int . Arch , Allergy. Immun. 112:115-124 .
14-Coalsen, J.J.; Junjii, H.H. and Mary, L.W. (1986). Morphologic and microbiologic features of P.aeruginosa Pneumonia in normal hamsters . Experm . and Moloc. Path. 45:193-602.
15-Tam, N.; Snipes, G.J. and Sterensan , M.M. (1999). Characterization of chronic bronchopulmonary P. aeruginosa infection in resistant and susceptible in brod mouse strains . Am. J. Respir. Cell Mol. Biol. 20;710-719.
16-AL-Kutbi, S.H. (2000) · Study on some aspects of pathogenesis of P. aeruginosa in rabbits M.Sc.. Thesis submitted to Coll. Vet. Med. University of Baghdad.
17-Choung , A.T. Mess, R.B. ; Kurland , G. andnevick , W.J. (1993) · Chrenic P. aeruginosa endobronchitis in rhesus monkeys 11. A histopathological analysis. J. Med. Primatol . 22:257-262. 18-Ramphal , R. and Pyle, M. (1983). Evidence for mucins and Sialic acid as receptor for P. aeruginosa in the lower repiratory tract infect. Immun. 83 : 500-504 .
19-Baker, J.R. ; Moyer, B.B. and Summer, A.T. (1973). Effect of chronic sulpher dioxide inhalation on the carbohydrato histochomistry. Am. Rev. Resp. Dis . 110:13-21.
20-Ganatwinon, A. and Korhnon, C.B. (1969) . The action of mucinin in promoting infection in goats . Brit. J. Exp. Path. 47: 355-357 .
21-Gibbons, R.A. (1959). Chemical properties of two mucoides from bovine cervical muein . Biochem. J. 73:209-214
22-Groften , J. and Douglas , A. (1981). Respiratory disease, 3rd edd. Oxford, London.