PATHOLOGICAL STUDY OF LOCALLY ISOLATED FOWL POXVIRUS -EXPERIMENTALLY INFECTED TRACHEA USING SCANNING ELECTRON MICROSCOPY
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Published:
Oct 3, 2021
Keywords:
POXVIRUS, TRACHEA, MICROSCOPY
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Abstract
In this study fowl poxvirus has been isolated from naturally infected birds. This virus induced respiratory distress, 2, 3 and 4 days post inoculation, in intratracheally inoculated 8 week old chickens, these respiratory distress include congestion of tracheal mucosa, increased tracheal secreation and whitish-yellowish pastules appeared at ninth day post inoculation in the upper part of the trachea forming diphtheritic or pseudodiphtheritic membrane. Using scanning electron microscopy to study the changes that occurs in the epithelial surface of trachea. It was found that the virus causes proliferation of epithelial cells forming cluster like papilloma, deciliation and activation of goblet cells.
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PATHOLOGICAL STUDY OF LOCALLY ISOLATED FOWL POXVIRUS -EXPERIMENTALLY INFECTED TRACHEA USING SCANNING ELECTRON MICROSCOPY. (2021). The Iraqi Journal of Veterinary Medicine, 26(1), 109-117. https://doi.org/10.30539/ijvm.v26i1.1126
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How to Cite
PATHOLOGICAL STUDY OF LOCALLY ISOLATED FOWL POXVIRUS -EXPERIMENTALLY INFECTED TRACHEA USING SCANNING ELECTRON MICROSCOPY. (2021). The Iraqi Journal of Veterinary Medicine, 26(1), 109-117. https://doi.org/10.30539/ijvm.v26i1.1126
References
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2. FAO (1992) Animal health yearbook.
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8. Griffiths, G.L. and Purcell, D.A.(1986) Fowl pox in the trachea of laying hens. Aust. Vet.J. 63:91-92.
9. Tripathy, D.N. and Reed, W.M.(1997) Pox. In: Diseases of Poultry. Calnek, B.W., Barnes, H.J., Beard,C.W.,McDougald,L.R. and Saif. Y.M. eds. 10 th ed. Iowa State University Press, Ames, Iowa. 643-659.
10. Al-Hyali, H.M. (2001a) Immunoenzyme detection of fowl poxvirus in cells of chorioallantoic membrane and trachea. Iraqi J. Vet. Sci. (accepted for publication).
11. Al-Hyali, H.M. (2001b) Use of immunoperoxidase and methylen blue staining techniques for detection of pathological changes of fowl poxvirus infected cell culture and tracheal epithelial cells. Iragi J. Vet. Sci. (accepted for publication).
12. Brooks, G.F., Butel,J.S., Ornston,L.N, Jawets,E., Melnick,J.I. and Adelbg, E. (1995) Medical Microbiology. 20" ed. Asimon and Schoster Company, USA, 381-390.
13. Mohammed, N.A.(1979) Comparative study of fowel pox and pigeon poxviruses. M.Sc. Thesis, College of Veterinary Medicine, University of Baghdad.
14. Al-Fahad, A.A.(1993) Fowel pox vaccine preparation on tissue cultural. SAM.Sc. Thesis, College of Veterinary Medicine, University of Baghdad.
15. Reed, L.J. and Muench. A.(1938) Simple method for estimating fifty percent endpoint. Am.J.Hyg. 27:493-497.
16. Buscaglia,C., Bankowski,R.A. and Miers, L.(1985) Cell cuture virus neutralization test and ELIZA for evaluation of immunity in chickens against fowl pox. Avian Dis. 29:672-678.
17. Minbay,A. and Kreier,J.P.(1973) An experimental study of the pathogenesis of fowl pox infection in chicken. Avian Dis. 17:532-539.
18. Al-Hyali. M.M., Al-Joboury. K.H. and Ismai. A.D. (1998) Pathological study on laryngotracheitis in layers. Iraqi. J. Vet. Sci. 22:50-66.
19. Austic, R.E. and Scott, M.L.(1997) Nutritional Diseases In: Diseases of Poultry. Calnek et al. 10th ed. Iowa State University Press, Ames, Iowa, 643-659.
20. Tanizaki, E., Kotani, T. and Odagiri, Y.(1987) Pathological changes of tracheal mucosa in chickens infected with fowl pox virus. Avian Dis. 31:169-175.
21. Bashoum,C.B.(1986) Regulation of airway secretory cell. Clin. Chest Med. 7: 231-237.
22. Hayashi,S. Odagiri, Y., Kotani, T. and Honochi, T.(1985) Pathological changes in the tracheal mucosa of chicken infected with ILT virus. Avian Dis. 29:943-950.
23. Lai, M.C. and Ibrahim, A.L.(1985) A study on a velogenic viscerotropic Newcastle disease virus in Vitro and in Vivo. Ph. D. Thesis, University Pertanian Malaysia, Selangor, Malaysia.
2. FAO (1992) Animal health yearbook.
3. Al-Falluji,M.M.; Tantawi,H.H.; Al-Bana, A. and Al-Sheikly,F.(1979) Pox infection among captive peacocks. J.Wild Dis. 15:597-600.
4. Tantawi, H.H; Al-Falluji,M.M. and Shony,M.O.(1979) Heat-selection mutants of pigeon poxvirus. Acta Virol. 23:249-252.
5. Al-Ani,M.O.(1986) An outbreak of pox among pheasants in Iraq. Avian Pathol. 15:795-796.
6. Trampel, D.W.(1996) Fowl pox update. In: Poultry news, Nebraska Poultry Newsletter (1997). Biannual Meeting of the Veterinarians, Iowa State University.
7. Aiello, E. and Mays, A.(1998) The Merck Veterinary Manual 8th ed. Whitehouse station, NJ.USA: 1960-1962.
8. Griffiths, G.L. and Purcell, D.A.(1986) Fowl pox in the trachea of laying hens. Aust. Vet.J. 63:91-92.
9. Tripathy, D.N. and Reed, W.M.(1997) Pox. In: Diseases of Poultry. Calnek, B.W., Barnes, H.J., Beard,C.W.,McDougald,L.R. and Saif. Y.M. eds. 10 th ed. Iowa State University Press, Ames, Iowa. 643-659.
10. Al-Hyali, H.M. (2001a) Immunoenzyme detection of fowl poxvirus in cells of chorioallantoic membrane and trachea. Iraqi J. Vet. Sci. (accepted for publication).
11. Al-Hyali, H.M. (2001b) Use of immunoperoxidase and methylen blue staining techniques for detection of pathological changes of fowl poxvirus infected cell culture and tracheal epithelial cells. Iragi J. Vet. Sci. (accepted for publication).
12. Brooks, G.F., Butel,J.S., Ornston,L.N, Jawets,E., Melnick,J.I. and Adelbg, E. (1995) Medical Microbiology. 20" ed. Asimon and Schoster Company, USA, 381-390.
13. Mohammed, N.A.(1979) Comparative study of fowel pox and pigeon poxviruses. M.Sc. Thesis, College of Veterinary Medicine, University of Baghdad.
14. Al-Fahad, A.A.(1993) Fowel pox vaccine preparation on tissue cultural. SAM.Sc. Thesis, College of Veterinary Medicine, University of Baghdad.
15. Reed, L.J. and Muench. A.(1938) Simple method for estimating fifty percent endpoint. Am.J.Hyg. 27:493-497.
16. Buscaglia,C., Bankowski,R.A. and Miers, L.(1985) Cell cuture virus neutralization test and ELIZA for evaluation of immunity in chickens against fowl pox. Avian Dis. 29:672-678.
17. Minbay,A. and Kreier,J.P.(1973) An experimental study of the pathogenesis of fowl pox infection in chicken. Avian Dis. 17:532-539.
18. Al-Hyali. M.M., Al-Joboury. K.H. and Ismai. A.D. (1998) Pathological study on laryngotracheitis in layers. Iraqi. J. Vet. Sci. 22:50-66.
19. Austic, R.E. and Scott, M.L.(1997) Nutritional Diseases In: Diseases of Poultry. Calnek et al. 10th ed. Iowa State University Press, Ames, Iowa, 643-659.
20. Tanizaki, E., Kotani, T. and Odagiri, Y.(1987) Pathological changes of tracheal mucosa in chickens infected with fowl pox virus. Avian Dis. 31:169-175.
21. Bashoum,C.B.(1986) Regulation of airway secretory cell. Clin. Chest Med. 7: 231-237.
22. Hayashi,S. Odagiri, Y., Kotani, T. and Honochi, T.(1985) Pathological changes in the tracheal mucosa of chicken infected with ILT virus. Avian Dis. 29:943-950.
23. Lai, M.C. and Ibrahim, A.L.(1985) A study on a velogenic viscerotropic Newcastle disease virus in Vitro and in Vivo. Ph. D. Thesis, University Pertanian Malaysia, Selangor, Malaysia.