Isolation and Identification of Lactobacillus salivarus to be used as probiotic in chickens
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Published:
Jun 28, 2003
Keywords:
Lactobacillus salivarus, chickens, Enrofloxacine
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Abstract
The study was conducted to isolate and identified Lactobacillus through its biochemical and some biological character
The growth inhibition of E. coli (EC) and Salmonella typhimurium (ST) invitro and its sensitivity to pH=3 and its antibiogram were studied.
Two isolates of Lactobacillus salivarus (LS) were obtained. The isolated bacteria were taken from the crops of adult chicken, and cultured on M.R.S. broth, they were Gram positive bacilli, negative for catalase and amonia production from Arginin. The first isolate showed inhibitory zone of 22 mm diameter against (EC) and 19 mm. against (ST) The second isolate showed inhibitory zone of 1mm diameter against (EC) and did not show an inhibitory zone against (ST)
Adhesive ability of the first isolate was strong while that of the second isolate was weak.
The first isolate showed growth on Rogosa media in pH=3 and
It was resistant to the following antimicrobial drugs: Enrofloxacine, Ciprofloxacine, Norfloxacine, Flumequine, Doxycycline, Tetracycline, Sulfanomide, Furaltidone, and sensitive to Ampicillin, Neomycin, Chloramphenicol.
It was concluded that the first isolate of Lactobacillus salivarus could be successfully used as probiotic in chickens.
The growth inhibition of E. coli (EC) and Salmonella typhimurium (ST) invitro and its sensitivity to pH=3 and its antibiogram were studied.
Two isolates of Lactobacillus salivarus (LS) were obtained. The isolated bacteria were taken from the crops of adult chicken, and cultured on M.R.S. broth, they were Gram positive bacilli, negative for catalase and amonia production from Arginin. The first isolate showed inhibitory zone of 22 mm diameter against (EC) and 19 mm. against (ST) The second isolate showed inhibitory zone of 1mm diameter against (EC) and did not show an inhibitory zone against (ST)
Adhesive ability of the first isolate was strong while that of the second isolate was weak.
The first isolate showed growth on Rogosa media in pH=3 and
It was resistant to the following antimicrobial drugs: Enrofloxacine, Ciprofloxacine, Norfloxacine, Flumequine, Doxycycline, Tetracycline, Sulfanomide, Furaltidone, and sensitive to Ampicillin, Neomycin, Chloramphenicol.
It was concluded that the first isolate of Lactobacillus salivarus could be successfully used as probiotic in chickens.
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How to Cite
Isolation and Identification of Lactobacillus salivarus to be used as probiotic in chickens. (2003). The Iraqi Journal of Veterinary Medicine, 27(1), 158-173. https://doi.org/10.30539/ijvm.v27i1.1106
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How to Cite
Isolation and Identification of Lactobacillus salivarus to be used as probiotic in chickens. (2003). The Iraqi Journal of Veterinary Medicine, 27(1), 158-173. https://doi.org/10.30539/ijvm.v27i1.1106
References
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2. السامرائي ، علي کريم (1993). تأثير العصيات اللبنية في إصابات الأكياس الهوائية في دجاج اللحم رسالة ماجستير ، كلية الطب البيطري ، جامعة بغداد.
3. Fuller, R. (1989). Probiotic in man and animals: A review. J. Appl. Bacteriol. 66:365 -378.
4. Fuller, R. and Turvey, A. (1971). Bacteria associated with the intestinal wall of the fowl. J. App. Bact. 34: 617-622.
5. Fuller, R. (1973). Ecological studies on the Lactobacillus flora associated with the crop epithelium of the fowl. J.Appl. Bacteriol. 36: 131 -139.
6. Fuller, R. and Brooker. (1974) Lactobacilli which attach to the crop epithelium of the fowl. Am.J.Clin.Nutr.27: 1305-1312.
7. Henriksson, A.; Szewzyk, R. and Conway, P.L. (1991). Characteristics of the adhesive determinants of Lactobacillus fermentum 104.Appl. Environ. Microbiol. 57:499-502
8. Tannock, G.W. (1987). Demonstration of mucosa – associated microbial population in the colons of mice. Appl. Environ. Microbiol. 53: 1965-1968
9. Gusils, C.; Perez, A.; Gonzalez, S. and Oliver, G. (1998). Lactobacilli isolated from chicken intestines: potential use as probiotics. Journal of Food protection Vol.62, No.3 Pages 252 -256.
10. Abdulrahim, S.M.; Haddadin, M.S.Y.; Hashlamoun, E.A.R. and Robinson, R.K. (1996). The influence of Lactobacillus acidophillus and Bactracin on layer performance of chickens and cholesterol content of plasm and egg yolk. Br.poult.Sci.37: 341-346.
11. Havenaar, R. and Huiint vedl, J. H. (1992). Probiotic: A general review. In: “ The lactic acid bacteria “. Wood, J. B. Ed. Blackie. Academic and professional Glasgow, UK
12. Hott, J.C. and Krieg, N. R. (1986). “Bergey's manual of determinative bacteriology“. Williams and Wilkins .Eds. 9th ed. Baltimore Maryland, U.S.A.
13. Haddadin, M. S. Y.; Abdulrahim, S. M.; Hashlmoun, E. A. R. and Robinson, R. K. (1997). A proposed protocol for checking the suitability of Lactobacillus acidophillus for use during feeding trials with chicken. Trop.Sci.37: 16-20.
14. Fuller, R. (1977). The importance of lactobacillus in maintaining normal microbial balance in the crop. Br. Poul. Sc. 18:85 – 94.
15. Al-Chalaby, Z.A.M.(1983). The ecology of Salmonella infection in chickens and it's influence on hygenic meat production. Ph.D. Thesis University of Bristol.
16. البياتي، لينا فلاح (2000). استخدام لقاح 9R في السيطرة على جراثيم السالمونيلا باراتيفوئيد في أفراخ الدجاج . رسالة ماجستير ، كلية الطب البيطري ، جامعة بغداد .
17. Rogosa, M. (1951). A selective medium for the isolation of oral and fecal lactobacilli. J. Bacteriol. 62: 132–133
18. DeMand, J. C.; Rogosa, M. and Sharpe, M. E. (1960). A medium for cultivation of lactobacilli. J. Appl. Bact.23: 130 -135
19. Harrigan, W.F.; Mccance, M. F. (1976). Laboratory methods in: Food and dairy microbiology. Academic press London.
20. Cupta, P.K.; Mital, B.K. and Grag, S.K. (1996). Characterization of Lactobacillus acidophilus for uses as dietary adjunct. International J. of food microbiology, Vol.29, No.7..
21. Stratford, B.C. (1977). Simple test of sensitivity (well diffusion technique). In: An atlas of medical microbiology pp. 138. Black well scientific publication. jell as ou quan aquel
22. Fuller, R. (1975). Nature of determinant responsible for adhesion of Lactobacilli to chicken crop epithelial cell. J.Gen.Micro.87: 245-250.
23. Chateau, N.; Gastellanos, I. and Deschamps, A.M. (1993). Distribution of pathology inhibition in the lactobacillus isolates of commercial Probiotic consortium. J. of Appl.Bacteriol.74:36-40
24. Cruickshank, R. (1975).”Medical Microbiology”. 12th. ed. Churchill Livingston. Edinburgh, London and New York.pp.199-200
25. Gilliland ,S.E.(1985). Bacterial starter culture for food CRS.press. Inc.Boca Rotan Florida U.S.A.
26. Farmer, J. J. 111. (1995). Enterobacteriaceae: introduction and identification. In: “ Manual of clinical microbiology“ (Baron, E. J.; Faller, M. A.P.; Tenover, F. C.; Yolken, R. H. Eds.). Pp. 438 – 449.
27. Barrow, P.A.; Brooker, B.E.; Fuller, R. and Newport, M.J. (1980). The attachment of bacteria to the gastric epithelium of the pig and its importance in the microecology of the intestine. J.Appl-Bacteriol.48: 147.
28. Sarra, P. G.; Morelli, L. and Bottazzi, V. (1992). The lactic acid microflora of fowl. In: “ The lactic acid bacteria in health and disease “ Wood, B.J. B Ed. Essex, UK: Elsevier Applied Science. Pp. 3-19.
29. Kandler, O. and Weiss, N. (1986). Genus lactobacillus. In: “ Bergey's Manual of Systemic Bacteriology“ Vol.2 (Sneath, P. H. A., Ed.) Williams and Wilkins Co., Baltimore, MD. PP.1208 -1234
30. Edens, F. W.; Parkhurst, C.R.; Cassas, I.A. and Dobrogosz, W.J. (1997). Principles of Ex-ovo competitive exclusion and in - ovo administration of Lactobacillus reuteri. Poult. Sci. 76:169-179.
31. Jernigan, M.A.; Miles, R.D. and Arafa, A.S. (1985). Probiotic in poultry nutrition -Areview. World's Poult.Sci.J. 41:99.
32. Shanhani, K. M.; Vakil, J. R. and Kilara, A. (1976). Natural antibiotic activity of lactobacillus acidophillus and L. bulgaricus. 1-Cultural condition for the production of antibiosis. Cult. Dair.Prod. J. 11: 14-17. ileg auton ajelangenisl elelläsjes awlw. (1999) is el toc volco Ch.
33. رسالة دكتوراه ، كلية الزراعة ، جامعة بغداد.
34. MacGroarty, J. A.; Rosanne, A. and Roger, L. Cook. (1998). Lactobacillus inhibitor production against E. coli Coaggregation ability with uropathogens. Can. J. Micro. 34: 344 –351;
35. Smulder, F. J.M.; Barendson, P.; Vanlogtestijn, J.G.; Mossel, D.A.A. and Yan Der Marel, G.M. (1986). Review: Consideration in favour of it's acceptance as meat decontamenant. J.Food Tech.21: 419-436.26.
36.Adams, M.R. and Hall, C.J. (1988). Growth inhibition of food – borne pathogens lactic and acetic acid and their mixtures. Int.J.Food.Sci.Techno.23: 287-292.
37. Bhowmik, T.; Johnson, M.C. and Ray, B. (1986). Cellular damage in dried Lactobacillus acidophillus. J. of Food prod. 49,No.1.
38. Sharpe, M.E., Fryer, T.F. and Smith, D.G. (1972). Identification of lactic acid bacteria .In: “Identification methods for microbiologist “ Skinner, F. A. ED. Academic press. London.
2. السامرائي ، علي کريم (1993). تأثير العصيات اللبنية في إصابات الأكياس الهوائية في دجاج اللحم رسالة ماجستير ، كلية الطب البيطري ، جامعة بغداد.
3. Fuller, R. (1989). Probiotic in man and animals: A review. J. Appl. Bacteriol. 66:365 -378.
4. Fuller, R. and Turvey, A. (1971). Bacteria associated with the intestinal wall of the fowl. J. App. Bact. 34: 617-622.
5. Fuller, R. (1973). Ecological studies on the Lactobacillus flora associated with the crop epithelium of the fowl. J.Appl. Bacteriol. 36: 131 -139.
6. Fuller, R. and Brooker. (1974) Lactobacilli which attach to the crop epithelium of the fowl. Am.J.Clin.Nutr.27: 1305-1312.
7. Henriksson, A.; Szewzyk, R. and Conway, P.L. (1991). Characteristics of the adhesive determinants of Lactobacillus fermentum 104.Appl. Environ. Microbiol. 57:499-502
8. Tannock, G.W. (1987). Demonstration of mucosa – associated microbial population in the colons of mice. Appl. Environ. Microbiol. 53: 1965-1968
9. Gusils, C.; Perez, A.; Gonzalez, S. and Oliver, G. (1998). Lactobacilli isolated from chicken intestines: potential use as probiotics. Journal of Food protection Vol.62, No.3 Pages 252 -256.
10. Abdulrahim, S.M.; Haddadin, M.S.Y.; Hashlamoun, E.A.R. and Robinson, R.K. (1996). The influence of Lactobacillus acidophillus and Bactracin on layer performance of chickens and cholesterol content of plasm and egg yolk. Br.poult.Sci.37: 341-346.
11. Havenaar, R. and Huiint vedl, J. H. (1992). Probiotic: A general review. In: “ The lactic acid bacteria “. Wood, J. B. Ed. Blackie. Academic and professional Glasgow, UK
12. Hott, J.C. and Krieg, N. R. (1986). “Bergey's manual of determinative bacteriology“. Williams and Wilkins .Eds. 9th ed. Baltimore Maryland, U.S.A.
13. Haddadin, M. S. Y.; Abdulrahim, S. M.; Hashlmoun, E. A. R. and Robinson, R. K. (1997). A proposed protocol for checking the suitability of Lactobacillus acidophillus for use during feeding trials with chicken. Trop.Sci.37: 16-20.
14. Fuller, R. (1977). The importance of lactobacillus in maintaining normal microbial balance in the crop. Br. Poul. Sc. 18:85 – 94.
15. Al-Chalaby, Z.A.M.(1983). The ecology of Salmonella infection in chickens and it's influence on hygenic meat production. Ph.D. Thesis University of Bristol.
16. البياتي، لينا فلاح (2000). استخدام لقاح 9R في السيطرة على جراثيم السالمونيلا باراتيفوئيد في أفراخ الدجاج . رسالة ماجستير ، كلية الطب البيطري ، جامعة بغداد .
17. Rogosa, M. (1951). A selective medium for the isolation of oral and fecal lactobacilli. J. Bacteriol. 62: 132–133
18. DeMand, J. C.; Rogosa, M. and Sharpe, M. E. (1960). A medium for cultivation of lactobacilli. J. Appl. Bact.23: 130 -135
19. Harrigan, W.F.; Mccance, M. F. (1976). Laboratory methods in: Food and dairy microbiology. Academic press London.
20. Cupta, P.K.; Mital, B.K. and Grag, S.K. (1996). Characterization of Lactobacillus acidophilus for uses as dietary adjunct. International J. of food microbiology, Vol.29, No.7..
21. Stratford, B.C. (1977). Simple test of sensitivity (well diffusion technique). In: An atlas of medical microbiology pp. 138. Black well scientific publication. jell as ou quan aquel
22. Fuller, R. (1975). Nature of determinant responsible for adhesion of Lactobacilli to chicken crop epithelial cell. J.Gen.Micro.87: 245-250.
23. Chateau, N.; Gastellanos, I. and Deschamps, A.M. (1993). Distribution of pathology inhibition in the lactobacillus isolates of commercial Probiotic consortium. J. of Appl.Bacteriol.74:36-40
24. Cruickshank, R. (1975).”Medical Microbiology”. 12th. ed. Churchill Livingston. Edinburgh, London and New York.pp.199-200
25. Gilliland ,S.E.(1985). Bacterial starter culture for food CRS.press. Inc.Boca Rotan Florida U.S.A.
26. Farmer, J. J. 111. (1995). Enterobacteriaceae: introduction and identification. In: “ Manual of clinical microbiology“ (Baron, E. J.; Faller, M. A.P.; Tenover, F. C.; Yolken, R. H. Eds.). Pp. 438 – 449.
27. Barrow, P.A.; Brooker, B.E.; Fuller, R. and Newport, M.J. (1980). The attachment of bacteria to the gastric epithelium of the pig and its importance in the microecology of the intestine. J.Appl-Bacteriol.48: 147.
28. Sarra, P. G.; Morelli, L. and Bottazzi, V. (1992). The lactic acid microflora of fowl. In: “ The lactic acid bacteria in health and disease “ Wood, B.J. B Ed. Essex, UK: Elsevier Applied Science. Pp. 3-19.
29. Kandler, O. and Weiss, N. (1986). Genus lactobacillus. In: “ Bergey's Manual of Systemic Bacteriology“ Vol.2 (Sneath, P. H. A., Ed.) Williams and Wilkins Co., Baltimore, MD. PP.1208 -1234
30. Edens, F. W.; Parkhurst, C.R.; Cassas, I.A. and Dobrogosz, W.J. (1997). Principles of Ex-ovo competitive exclusion and in - ovo administration of Lactobacillus reuteri. Poult. Sci. 76:169-179.
31. Jernigan, M.A.; Miles, R.D. and Arafa, A.S. (1985). Probiotic in poultry nutrition -Areview. World's Poult.Sci.J. 41:99.
32. Shanhani, K. M.; Vakil, J. R. and Kilara, A. (1976). Natural antibiotic activity of lactobacillus acidophillus and L. bulgaricus. 1-Cultural condition for the production of antibiosis. Cult. Dair.Prod. J. 11: 14-17. ileg auton ajelangenisl elelläsjes awlw. (1999) is el toc volco Ch.
33. رسالة دكتوراه ، كلية الزراعة ، جامعة بغداد.
34. MacGroarty, J. A.; Rosanne, A. and Roger, L. Cook. (1998). Lactobacillus inhibitor production against E. coli Coaggregation ability with uropathogens. Can. J. Micro. 34: 344 –351;
35. Smulder, F. J.M.; Barendson, P.; Vanlogtestijn, J.G.; Mossel, D.A.A. and Yan Der Marel, G.M. (1986). Review: Consideration in favour of it's acceptance as meat decontamenant. J.Food Tech.21: 419-436.26.
36.Adams, M.R. and Hall, C.J. (1988). Growth inhibition of food – borne pathogens lactic and acetic acid and their mixtures. Int.J.Food.Sci.Techno.23: 287-292.
37. Bhowmik, T.; Johnson, M.C. and Ray, B. (1986). Cellular damage in dried Lactobacillus acidophillus. J. of Food prod. 49,No.1.
38. Sharpe, M.E., Fryer, T.F. and Smith, D.G. (1972). Identification of lactic acid bacteria .In: “Identification methods for microbiologist “ Skinner, F. A. ED. Academic press. London.