Comparison Between The Efficacy Of Different Laboratory Methods For Diagnosis Of Cryptosporidium
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Published:
Jun 28, 2004
Keywords:
Cryptosporidium, methylene blue, safranin methylene blue
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Abstract
This study was performed to detect the effective laboratory method for diagnosis of Cryptosporidium spp. in patients attending the laboratories of Al-Tameem General Hospital and Kirkuk Paediatric Hospital in Kirkuk city, for the period from the beginning of January to end of August 2000..
It was shown that the modified Ziehl-Neelsens stain (hot method) with methylene blue (counter stain) was most efficient in identification of Cryptosporidium oocysts (4.36%), followed by malachite green (counter stain) (1.94%) and modified Ziehl-Neelsen (cold method) with malachite green (counter stain), safranin methylene blue, lugol s iodine preparation (mounting) (0.97%) for each and the modified Ziehl-Neelsen stain (cold method) with methylene blue (counter stain) was the least efficient (0.84%). .
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Comparison Between The Efficacy Of Different Laboratory Methods For Diagnosis Of Cryptosporidium. (2004). The Iraqi Journal of Veterinary Medicine, 28(1), 244-256. https://doi.org/10.30539/ijvm.v28i1.1084
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How to Cite
Comparison Between The Efficacy Of Different Laboratory Methods For Diagnosis Of Cryptosporidium. (2004). The Iraqi Journal of Veterinary Medicine, 28(1), 244-256. https://doi.org/10.30539/ijvm.v28i1.1084
References
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11.Al-Yassin, S.T. (1999) A study of cryptosporidiosis in immunocomprimised patients. MSc thesis, College of Medicine, Tikrit Univ.
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13.Haumouda, NA, Sadaka, HA, El-Gebaly, WM and El-Nassery, SM. (1996) Opportunistic intestinal protozoa in chronic diarrhoeic immunosuppressed patients. J. Egypt. Soc. Parasitol.: 26, 143-53.
14.Baxby, D., Hart, CA, Taylor, C. (1983) Human cryptosporidiosis a possible case of hospital cross infection. Br. Med. J., 278, 1760 1761.
15. Youssef, MY, Khalifa, AM and El-Azzonni, MZ (1998) Detection of Cryptosporidia in different water sources in Alexandria by monoclonal antibody test and modified Ziehl-Neelsen stain. J. Egypt. Soc. Parasitol.: 28, 487-96. 16.Manabe, YC, Clark, DP, Moore, RD. (1998) Cryptosporidium in patient with AIDS: correlates of disease and survival. Clin. Infect. Dis., Cited in Markell, John Krotosk, 1999.
2. Ma, P. and Soave, R. (1983) Three steps-.stool examination for cryptosporidiosis in ten homosexual men with protracted watery diarrhoea”. J. Inf. Dis.: 147, 824-828.
3. Baxby D, Blundell, N and Hart, CA (1984) The development and performance of simple sensitive method for the detection of Cryptosporidium oocysts in faeces. J. Hyg.: 02, 317-23.
4. Payne, P., Lancaster, LA, Heinzman, M., and McCutchan, JA. (1983) Identification of Cryptosporidium in patient with the acquired immunodeficiency syndrome. N. Engl. J. Med.: 309,
613-14.
5. Markell, E.K., Voge, M. and John, D.T. (1986.) Medical Parasitology. 6 th. Edit. , Philadelphia, W.B. Saunders Co.: 331 337.
6. Ignatius, R; Lehmann, M; Miksits, K; Prgnath, T; Arvand, M Engelmann, E and Wagner, J. (1997) A new acid fast trichrome stain for simultaneous detection of Cryptosporidium parvum and microsporidial species in stool specimens. J. Clin.Microbiol:35, 446-9.
7. Brown, SM, McDonald, V, Denton, H. and Coombs, GH. (1996) The use of a new viability assay to determine the suceptibility of Cryptosporidium and Eimeria sporozoites to to respiratory inhibitors and extremes of pH. FEMS Microbiol. Lett: 142, 203 208.
8. Flec, S.L. and Moody, A.H. (1988). Diagnostic technique in medical parasitology. 1 st. edition Great Britain, Butterworth Co.: 13.
9. World Health Organization. (1991) Basic laboratory method in medical parasitology. Geneva: 9-31.
10.Henrikson, S.A. and Pohlenz, J.F.L. (1981) Staining of Cryptosporidium by a Modified Ziehl-Neelsen technique. Acta Vet. Scand.: 22, 594-96.
11.Al-Yassin, S.T. (1999) A study of cryptosporidiosis in immunocomprimised patients. MSc thesis, College of Medicine, Tikrit Univ.
12. Ignatius, R., Elisenblatter, M., Regnath, T. (1997) Efficacy of different methods of low Cryptosporidium parvum oocysts numbers or antigen concentration in stool specimens. Eur. J. Clin. Microbiol. Infect. Dis.: 16, 732-6.
13.Haumouda, NA, Sadaka, HA, El-Gebaly, WM and El-Nassery, SM. (1996) Opportunistic intestinal protozoa in chronic diarrhoeic immunosuppressed patients. J. Egypt. Soc. Parasitol.: 26, 143-53.
14.Baxby, D., Hart, CA, Taylor, C. (1983) Human cryptosporidiosis a possible case of hospital cross infection. Br. Med. J., 278, 1760 1761.
15. Youssef, MY, Khalifa, AM and El-Azzonni, MZ (1998) Detection of Cryptosporidia in different water sources in Alexandria by monoclonal antibody test and modified Ziehl-Neelsen stain. J. Egypt. Soc. Parasitol.: 28, 487-96. 16.Manabe, YC, Clark, DP, Moore, RD. (1998) Cryptosporidium in patient with AIDS: correlates of disease and survival. Clin. Infect. Dis., Cited in Markell, John Krotosk, 1999.