COMPARISON OF DIFFERRENT LABORATORY METHODS FOR DIAGNOSIS OF CRYPTOSPORIDIUM
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Published:
Nov 1, 2021
Keywords:
LABORATORY METHODS, CRYPTOSPORIDIUM
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Abstract
The present study was carried on to compare several methods for identification of Cryptosporidium oocysts sp. which conclude modified acid fast stain, Sheather's flotation solutions, Ritchi formal-ether sedimentation solutions, noval stain and iodine technique. The modified acid-fast stain and its combination with formal – ether technique gave the best demonstration 100%. The most reliable technique for identification of oocysts was formal-ether concentration combination with modified acid fast stain (100%), followed by using modified acid fast stain only (97%), sucrose flotation technique (90%) novel staining technique (85%), formal ether concentration (84%), and iodine technique (69%) respectively.
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COMPARISON OF DIFFERRENT LABORATORY METHODS FOR DIAGNOSIS OF CRYPTOSPORIDIUM. (2021). The Iraqi Journal of Veterinary Medicine, 26(1), 153-158. https://doi.org/10.30539/ijvm.v26i1.1134
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How to Cite
COMPARISON OF DIFFERRENT LABORATORY METHODS FOR DIAGNOSIS OF CRYPTOSPORIDIUM. (2021). The Iraqi Journal of Veterinary Medicine, 26(1), 153-158. https://doi.org/10.30539/ijvm.v26i1.1134
References
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2. Tzipori S. (1998) Cryptosporidiosis: laboratory investigations and chemotherapy. Adv. Parasitol, 40: 187-94.
3. Miller R.; Holmberg R. and Clusen C. (1983) Life-threatening diarrhea caused
by Cryptosporidium in child undergoing therapy for acute leukemia. J. Pediatr., 103:256-59.
4. Mackenzie W. R.; Hoxie N. J.; Proctor ME; Grades M. S.; Blaire K.A.; and
Peterson D.F.(1994)A massive outbreak in Milwaukee of Crytptosporidium infection transmitted through the public water supply. N.Eng.J.Med,331,161 67.
5. Casemore D.P.(1989) Human cryptosporidiosis, Recent advance in infection. 3: 209-236.
6. Moon H. W. & Bemrick W. J. (1981) Fecal transmission of calf cryptosporidiosis between calves and pigs. Vet. Pathol., 18: 248-255.
7. Heinemann D.(1987) Medical parasitology. In: Jawitz E., MeinikJ. L., Adelberg E. A.(Eds.) Review of Medical Microbiology, 17 Edition,
Washington Appleton & Long Co. 553. :
8. Bennson A. S.(1995) Control of Communicable Diseases Manual. 16 edition Washington, American Public Health. 121.
9. Pavlasek I. Nikitin V. F. (1984) (Eimeria in calves on industrial farms]. Veterinariya, Moscow; 5:44-45..