Survey and Molecular Study of Babesia gibsoni in Dogs of Baghdad Province, Iraq
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Abstract
This study aimed to detect Babesia gibsoni (B. gibsoni) in dogs of different ages, sex and breeds in Baghdad province by microscopic and molecular investigations using polymerase chain reaction (PCR), sequencing, and phylogenetic analyses. The present study was investigated B. gibsoni in 310 blood samples of dogs for the period from December 2018 to September 2019 in Baghdad province, Iraq. The molecular study was carried out by using universal primers of Babesia spp. (PIRO-A and PIRO-B) and specific primers of B. gibsoni (BAGIF and BAGIR) products size of 410 bp and 488 bp fragments of 18S rRNA gene respectively. The clinical signs revealed higher percentage and specific clinical signs of B. gibsoni as depression, anorexia, fever, pale mucus membrane, and ticks infestation, however icterus, and dead were low in which only occurred in two dogs out of infected dogs. The PCR assay and microscopic diagnosis revealed the infection rate of B. gibsoni 9 out of 310 (2.9%) in dogs. The sequence data analyses of nine DNA products were 98-100% similar to sequences of 18S rRNA gene of B. gibsoni data available in Gene bank. According to breed, age, and sex, the results revealed a significantly high-risk factor of infection in Husky dogs; B. gibsoni detected in females which was increased non-significantly than males; while the highest occurrence of disease was in young dogs aged three years or less in addition to the above, the infection rate of B. gibsoni was high in spring season. In conclusion, this study was considered the first molecular record of B. gibsoni in Baghdad, Iraq documented no differences in diagnosis by blood smear and conventional PCR to amplify of 18S rRNA gene and partial sequencing of B. gibsoni with low-cost method and easily done.
Correspondence: [email protected]
Received: 9 March 2020
Accepted: 21 Jun 2020
Published: 28 December 2020
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Jalali MHR, Mosallanejad B, Avizeh R, Alborzi AR, Nejat HH, Taghipour R. Babesia infection in urban and rural dogs in Ahvaz district Southwest of Iran. Arch Razi Inst. 2013; 68(1): 37-42.
Birkenheuer AJ. Babesiosis In: Infectious diseases of the dog and cat. 4th ed. edited by Greene CE Elsevier; 2012. 771-784 p.
Singh A, Singh H, Singh NK, Singh ND, Rath SS. Canine babesiosis in Northwestern India: molecular detection and assessment of risk factors. Biomed Res Int. 2014; (741785): 1-5.
Taylor MA, Coop RL, Wall RL. Veterinary parasitology. 4th ed. Wiley Blackwell UK; 2016. 152 p.
Birkenheuer AJ, Levy MG, Breitschwerdt EB. Development and evaluation of a seminested PCR for detection and differentiation of Babesia gibsoni (Asian genotype) and B canis DNA in canine blood samples. J Clin Microbiol. 2003; 41(9): 4172-4177.
Goo Y and Xuan X. New molecules in Babesia gibsoni and their application for diagnosis vaccine development and drug discovery Korean. J parasitol. 2014; 52(4): 345-353.
Matjila PT, Penzhorn BL, Leisewitz AL, Bhoora R, Barker R. Molecular characterization of Babesia gibsoni infection from a Pit-bull terrier pup recently imported into South Africa. J South African Vet Asso. 2007; 78(1): 2-5.
Lee MJ, Yu DH., Yoon JS, Li YH, Lee JH, Chae JS, Park J. Epidemiologic and clinical surveys in dogs infected with Babesia gibsoni in South Korea. Vector-Borne Zoonotic Dis. 2009; 9(6): 681-686.
Imre M, Farkas R, Stelian M, Imre K, Gheorghe D. Ticks and tick-borne diseases survey of babesiosis in symptomatic dogs from Romania: Occurrence of Babesia gibsoni associated with breed. Ticks and Tick-borne Dis. 2013; 4: 500-502.
Inokuma H, Yoshizaki Y, Matsumoto K. Molecular survey of Babesia infection in dogs in Okinawa Japan. Vet Parasitol. 2004; 121(3-4): 341-346.
Akhtardanesh B, Saberi M, Nurollahifard SR, Aghazamani M. Molecular detection of Babesia spp in tick-infested dogs in southeastern. Iran J Dis Glob Heal. 2016; 8(2): 72-77.
Aysul N, Ural K, Ulutaş B, Eren H, Karagenç T. First detection and molecular identification of Babesia gibsoni in two dogs from the Aydın Province of Turkey. Turkish J Vet Anim Sci. 2013; 37(2): 226-229.
Mittal M, Kundu K, Chakravarti S, Mohapatra JK, Singh VK., Kumar BR, Thakura V, Churamania CP, Kumar A. Canine babesiosis among working dogs of organized kennels in India: A comprehensive haematological biochemical clinicopathological and molecular epidemiological multiregional study. Prev Vet Med. 2019; 169(104696): 1-9.
Davitkov D, Vucicevic M, Stevanovic J, Krstic V, Tomanovic S, Glavinic U, Stanimirovic Z. Clinical babesiosis and molecular identification of Babesia canis and Babesia gibsoni infections in dogs from Serbia. Acta Vet Hung. 2015; 63(2): 199-208.
Jain KJ, Lakshmanan B, Karunakaran Syamala JEP, Aravindakshan T. High prevalence of small Babesia species in canines of Kerala South India. Vet World. 2017; 10(11): 1319-1323.
Jain J, Lakshmanan B, Nagaraj HV, Praveena JE., Syamala K, Aravindakshan T. Detection of Babesia canis vogeli Babesia gibsoni and Ehrlichia canis by multiplex PCR in naturally infected dogs in South India. Vet Arh. 2018; 88(2): 215-224.
Arsalan SH. Clinical haematological and biochemical studies of some blood protozoa in dogs in Nineveh. Iraqi J. Vet. Sci. 2005;1(19): 63-77.
Al-taie DA and Fadhil AI. Incidence of Babesia gibsoni in stray dogs (Iraq). Online J Vet Res. 2013;17(1): 32-35.
Voytas D. Agarose gel electrophoresis. Curr Protoc Mol Biol. 2000; 51(1): 2-5.
He L, Miao X, Hu J, Huang Y, He P, He J. First molecular detection of Babesia gibsoni in dogs from Wuhan China. Frontiers in Microbiol. 2017; 8(1577): 1-6.
Mahalingaiah MKC, Asoor M, Thimmaiah RP, Narayanaswamy HD, Mukartal SY, Elattuvalappil AM, Singh S. Prevalence of canine babesiosis in different breeds of dogs in and around Bengaluru. Adv Anim Vet Sci. 2017; 5(3): 140-144.
Laha R, Bhattacharjee K, Sarmah PC, Das M, Goswami A, Sarma D, Sen A. Babesia infection in naturally exposed pet dogs from a north-eastern state (Assam) of India: detection by microscopy and polymerase chain reaction. J Parasit Dis. 2014; 38(4): 389-393.
Chandra BS, Rajkumar K, Thanislass J, Vijayalakshmi P, Abiramy A. Molecular diagnosis of Babesia gibsoni Infection. Int J of Lives Res. 2018; 8(09): 98-103.
Lee CC, Hsieh YC, Huang CC, Tsang CL and Chung YT. Sequence and phylogenetic analysis of the thrombospondin-related adhesive protein (TRAP) gene of Babesia gibsoni isolates from dogs in Taiwan. J Vet Med Sci. 2010; 72(10): 1329-1335.
Düzlü Ö, Abdullah İ, Yildirim A, Önder Z, Çiloğlu A. The investigation of some tick-borne protozoon and rickettsial infections in dogs by real time PCR and the molecular characterizations of the detected isolates. Ankara Üniversitesi Vet Fakültesi Derg. 2014; 61(4): 275-282.
Kubo S, Tateno M, Ichikawa Y, Endo Y. A molecular epidemiological survey of Babesia Hepatozoon Ehrlichia and Anaplasma infections of dogs in Japan. J Vet Med Sci. 2015; 77(10): 1275-1279.
Terao M, Akter S, Yasin MG, Nakao R, Kato H, Alam MZ, Katakura K. Molecular detection and genetic diversity of Babesia gibsoni in dogs in Bangladesh. Infect Genet Evol. 2015; 31: 53-60.
Barash NR, Thomas B, Birkenheuer AJ, Breitschwerdt EB, Lemler E, Qurollo BA. Prevalence of Babesia spp. and clinical characteristics of Babesia vulpes infections in North American dogs. J Vet Intern Med. 2019; 33(5): 2075-2081.
Sarma K, Nachum-Biala Y, Kumar M, Baneth G. Molecular investigation of vector-borne parasitic infections in dogs in Northeast India. Parasit Vectors. 2019; 12(1): 1-8
Yao D, Jiang J, Yu Z, Yao DQ, Yang DJ, Zhao Y. Canine babesiosis in China caused by Babesia gibsoni: a molecular approach. Iran J Parasitol. 2014; 9(2): 163-168.
Fadhil AI. Prevalence study of hard tick Rhipicephalus sanguineus in stray dogs form many areas from Baghdad province. Iraqi J. Vet. Med. 2014; 38(2): 9-11.