Genetic Analysis of Field Isolates of Infectious Bursal Disease Virus in Iraqi Farms
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Abstract
Sixty samples of bursa of Fabricius were collected from broiler chickens suspected to be infected with infectious bursal disease virus (IBDV) in different areas of Iraq for molecular evaluation. The extracted nucleic acid was amplified using reverse transcriptase polymerase chain reaction (RT-PCR) targeting genes of segment A (Vp2, Vp3, Vp4 and Vp5 genes) and segment B (VP1 genes). The products of amplification were sent to Korea for sequencing using Sanger method. The sequencing analysis of the IBDV from the Iraqi isolates revealed that each gene had different transition and transversion (nonsense and missense of point mutation) compared to reference genes. The phylogenetic tree analysis showed that the VP2 of segment A of the Iraqi samples was similar to that of an Egyptian strain with 96%similarity, the polypeptide VP2-3-4 of segment A of the Iraqi samples was similar to those of a Chinese strain with 99% similarity and the VP5 of segment A was similar to that of Chinese strain with 99% similarity. However, the phylogenetic tree analysis showed that the VP1 of segment B had 95% similarity with that of a Chinese strain.
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Eterradossi N, Saif, YM. Infections Bursal Disease. In: Swayne DE (ed) Diseases of Poultry, 13th ed.
Wiley-Blackwell, Ames; 2013.
Ferrero D, Garriga D, Navarro A, Rodríguez JF, Verdaguer N. Infectious bursal disease virus VP3
upregulates VP1-mediated RNA-dependent RNA replication. J Virol. 2015;89(21): 11165-8.
Wu H, Shi L, Zhang Y, Peng X, Zheng T, Li Y, et al . Ubiquitination is essential for avibirna virus replication
by supporting VP1 polymerase activity. J virol. 2019; 93(3): e01899-18.
Le XTK, Doan HTT, Do RT, Le TH. Molecular characterization of field isolates of infectious bursal disease
virus from three decades, 1987–2018, reveals a distinct genotypic subgroup in Vietnam. Arch Virol. 2019;1-9.
Wang W, Song Y, Liu L, Zhang Y, Wang T, Zhang W, et al. Neutralizing antibody mediated protection
of chickens against infectious bursal disease via one-time vaccination with inactivated recombinant
Lactococcus lactis expressing a fusion protein constructed from the RCK protein of Salmonella enterica
and VP2 of infectious bursal disease virus. Microb. Cell Fact. 2019;18(1): 21
Luque D, Saugar I, Rejas MT, Carrascosa JL, Rodríguez JF, Castón JR. Infectious bursal disease virus: ribonucleoprotein complexes of a double-stranded RNA virus. J M Biol. 2009;386(3): 891-901.
Lee CC, Wu CC, Lin TL. Chicken melanoma differentiation-associated gene 5 (MDA5) recognizes infectious bursal disease virus infection and triggers MDA5-related innate immunity. Arch Virol. 2014;159(7): 1671-86.
Lejal N, Da-Costa B, Huet JC, Delmas B. Role of Ser-652 and Lys-692 in the protease activity of infectious
bursal disease virus VP4 and identification of its substrate cleavage sites. J Gen Virol. 2000;81(4): 983-92.
Méndez F, Romero N, Cubas LL, Delgui LR, Rodríguez D, Rodríguez JF. Non-lytic egression of infectious
bursal disease virus (IBDV) particles from infected cells. PloS one. 2017;12(1):e0170080.
Li Z, Wang Y, Xue Y, Li X, Cao H, Zheng SJ. Critical role for voltage-dependent anion channel 2 in infectious bursal disease virus-induced apoptosis in host cells via interaction with VP5. J virol. 2012;86(3):1328-38.
Li K, Courtillon C, Guionie O, Allée C, Amelot M, Qi X, et al. Genetic, antigenic and pathogenic
characterization of four infectious bursal disease virus isolates from China suggests continued evolution
of very virulent viruses. Infec Genet Evol. 2015;30: 120-7.
Pikuła A, Domańska BK, Cepulis R, Śmietanka K. Identification of infectious bursal disease virus with atypical VP2 amino acid profile in Latvia. J Vet Res. 2017;61(2): 145-9.
Jackwood DJ, Sommer WSE, Crossley BM, Stoute ST, Woolcock PR, Charlton BR. Identification and pathogenicity of a natural reassortant between a very virulent serotype 1 infectious bursal disease virus
(IBDV) and a serotype 2 IBDV. Virol. 2011;420(2): 98-105.
Abed M, Soubies S, Courtillon C, Briand FX, Allée C, Amelot M, et al. Infectious bursal disease virus in
Algeria: Detection of highly pathogenic reassortant viruses. Infect, Genet Evol.2018;60: 48-57.
Soubies SM, Courtillon C, Briand FX, Queguiner-Leroux M, Courtois D, Amelot M, et al. Identification of a European interserotypicreassortant strain of infectious bursal disease virus. Avian Pathol. 2017;46(1): 19-27.
Pikuła A, Lisowska A, Jasik A, Śmietanka K. Identification and assessment of virulence of a natural
reassortant of infectious bursal disease virus. J Vet Res. 2018; 49(1):89.
Yousif NK. Isolation and study of Gumboro virus in Iraq [Thesis]. Baghdad, Iraq, University of Baghdad; 1995. 18. Nawzad AR, Nahla MS, Shilan FM, Zana HM. Detection and Identification of Infectious Bursal Disease
Virus in Broiler Farms in Sulaimani Province. Intl Journal of Adv in ChemEng and Biol Sci (IJACEBS).
;3(1):39-43.
Mohammed MH, Ali BH, Hasan II. Molecular characterization of local isolation of infectious bursal disease virus from broiler chickens in Iraq. Malays Appl Biol. 2015;43(2): 97–103.
Al-Zuhariy MTB, Rabee RHS, Abdulmaged SH, Al-Baldawi AAA. Molecular Genotyping of Infectious Bursal Disease virus (IBDV) isolated from layer Flocks in Iraq. Al-Anbar J. Vet. Sci. 2016;(2): 100–9.
Caston JR, Rodriguez JF, Carrascosa JL. Infectious Bursal Disease Virus (IBDV) segmented double stranded RNA viruses: Structure and Molecular Biology.2008;355-400.
Zierenberg K, Nieper H, Van den Berg TP, Ezeokoli CD, Voss M, Müller H. The VP2 variable region of African and German isolates of infectious bursal disease virus: comparison with very virulent, classical, virulent, and attenuated tissue culture-adapted strains. Arch Virol. 2000;145(1): 113–25.
He X, Wei P, Yang X, Guan D, Wang G, Qin A. Molecular epidemiology of infectious bursal disease viruses isolated from Southern China during the years 2000–2010. Virus Genes. 2012;45(2): 246–55.
Ebersberger I, Metzler D, Schwarz C, Pääbo S. Genomewide comparison of DNA sequences between
humans and chimpanzees. Am J Hum Genet. 2002;70(6): 1490–7.
Cristina J. The Ecological Role of Micro-organisms in the Antarctic Environment, switsland Springer, Cham, 2019. Chapter9., Molecular Biology of RNA Viruses Isolated in Antarctica; P.197-217.
Zhou T, Ko EA, Gu W, Lim I, Bang H, Ko JH. Non-silent story on synonymous sites in voltage-gated ion channel genes. Plos One. 2012;7(10): 485.
Shi L, Li H, Ma G, Zhou J, Hong L, Zheng X, et al . Competitive replication of different genotypes of infectious bursal disease virus on chicken embryo fibroblasts. Virus Genes. 2009;39(1): 46–52.
Yilmaz A, Turan N, Bayraktar E, Gurel A, Cizmecigil UY, Aydin O, et al. Phylogeny and evolution of infectious bursal disease virus circulating in Turkish broiler flocks. J Poult Sci. 2019;98(5): 1976-84.
Rudd MF, Heine HG, Sapats SI, Parede L, Ignjatovic J. Characterisation of an Indonesian
very virulent strain of infectious bursal disease
virus. Arch Virol. 2002; 147:1303–1322.