Evaluation of Toxicity and Antimicrobial Activity of Watery and Alcoholic extracts of some plant on growth of pathogenicBacteria isolatedfrom diarrhea
Main Article Content
Abstract
In This study, the bacteria Escherichia Coli, Salmonella typhimurium and Shigella dysentarea were isolated from diarrhea in infants. Microbiological and biochemical tests were conducted to identify these bacteria. In the Identification of bacterial Species the API-System was used. Sensitivity test of bacterial isolates revealed high resistance to many Antibiolics like Ampicillin, Tetracycline, Ceftazidine, Cephalothin ,Vancomycin and Rifamipicin.
The effects of extracts of (Citrus aurantifolia ,Zingiber offienals, Glycyrrhiza glabra, Pimpinellaanisum, Camellia ,sinessis Coffeiaarabica, Elattariacar domomum, Cumminum cumminum, Thymus vulgaris and Trigonella foenum geoecum in inhibiting bacteria isolated from diarrhea were studied , also indication of presence of chemical active components in extracts was observed . The Preliminary chemical tests revealed acidic PH of all extracts, and the best antibacterial was effect that of Citrus aurantifolie an lcoholic extract on growth of E.coli with inhibition zone diameter 47mm.
Downloads
Article Details
How to Cite
References
-Victora, CG.;Kirkwood, BR.; fuchs, SC.; Lombardi, C. and Barros, FC. (1990).Is
itpossible, to predict which diarrhoea episodes will lead to life Threatening dehydration
.Int J.Epidemiol., 19:736-743.
-Zanbransky, RJ.(1997).Infectious disease prime cause of death
worldwide.Clin.Microbiol.News Letter, 19:7-8.
-Mata, L.; Urrutia, JJ.andSimhon,A. (1984). Infectious Agents in Acute and chronic
diarrhea of childhood.In:chronic diarrhea in children. Edited by EmanualLebenthal
,Raven press, New York.Pp: 237-251.
-Roderick, P.; Weeler, J.; Cowden, J.;Sockett, P.; Skinner, R.; Mortmer, P. andRawe B and
Rodriques, L.(1995).Apilot study of infectious intestinal disease in England,
Epidermiol.Infect. 11: 227-288.
-Branksi, D.; Lerner, A. and Lebenthal, E. (1996).Chronic diarrhoea
amalabsorptionpediater.Gastroenterol. 43(2):307-331.
-Kaplan, LA.and Pesce, AJ. (1989).Clinical chemistry. Theory, anaylsis and correlation.
The C.V. Mosby comp.USA .
-Henry, JB.(2001).Clinical diagnosis and management by .Laboratory Methods. 20thed.,
WB.Saunderscompany,U.S.A.
-Jawetz, E.; Melnick, JL.and Adelberg, A.(1998).Review of Medical Microbiology 21 th
ed. Middle eastedition, Beirut,Lebanon.
-Collee, JG.; Franser, AG.;Marmion, BP. and Sinmones, A. (1996).Mackie and
Maccartney Practical Medical Microbiology 14 thed. Churchill LivingstoneLondon,
Pp:97-133.
-Kirby, WM. andBauer, AM.(1966). Antibiotic susceptibility testing by a standardized
single disc method J.clin.Pathol.,45:493-496
-Swanston, FS.; Day, C.; Baileg, CJ.andFlatt, PR. (1990).Tradional plant treatments For
diabetes. Studies in normal and Streptozatocin diabetic mice.Diabetologia., 33:462-464.
-Harborn, JB.; Mabary, TJ.andMabary, H. (1975).Physiological and Functional of
Flavonoids.NewYork,SanFrancisco, p:970.
- Prescott,LM.;Harley, JP.and Klein, DA.(1993). Microbiology; 2nded. W.M.C.Brown.
Publishers London Chicago, p: 89.
- Vandepitte, J.;Engback, K.; Piot, P. and Heuk, C.(1991).Basic Laboratory Procedures in
clinical bacteriology World Health Organization,2nded. Geneva.
-Klassan, CD.;Amdar, MO. and Doul, IJ.(1986).Casarett and Doulls toxicology the basic
science of poison.3rd ed.macmillon publishing company, NewYork.
-Green field, J. and Bigland, CH.(1971).Comparison of the rates of motility of
salmonehha with those of other enteric bacteria Appl. Microb., 21(2):240-243.
- Murray, RG. ; Brenner, DJ.;Bryant, MP.; Halt, JG.; King, NR.; Moulder, JW.;Pfenning,
N.;Sneath, P.and HandStaley, JT. (1984).Bergeys manual of systemic bacteriology,
vol.1willians&wilkins.Baltjimore. London, Pp:408-446.
- Al-Rawi, A. and Chakavarty, HL.(1988).Medicinal plants of Iraq.2nded.Ministry of
Agriculture and Irrigation Baghdad-Iraq.
- Taylor, GD.; Buchana, CM.; Kirkland, T.;Mckenzzic, M. and Weins,
R.(1992).Bacteremic nosocomial pneumoniae-A7.experience in one
instituationchest.,168;786-788.