Molecular surveillance and phylogenetic analysis of Theileria annulata in bovine at Baghdad city/ Iraq
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Abstract
This study is conducted to investigate Theileria spp. by traditional and molecular methods. A total of 150 blood and 50 lymph samples were collected from local breed symptomatically and asymptomatically cattle of both sexes with age ranging from less than 6 months to more than 1 year during the four seasons of 2018, in different parts at Baghdad city / Iraq. Microscopic examination of Giemsa stained blood smears revealed 39.33 %( 59/150) rate of infection with bovine theileriosis and 34 %( 17/50) positive lymph smears. Statistically no significant difference recorded between female and male: 42.04 % (37/88) and 35.48 % (22/62) respectively. Higher rate of infection 57.97 % (40/69) were recorded in more than 1 year age and 0 % in less than 6 months. 48.93 % (23/47) rate of bovine theileriosis was recorded during summer and 39.53 % (17/43) ; 37.5 % (15/40) rates were recorded during spring and autumn respectively, while the lower rate recorded in winter 20 % (4/20). DNA extraction and polymerase chain reaction (conventional PCR) were done on all cattle blood samples the result recorded that 22 out of 25 samples were positive for Theileria spp and Theileria annulata with percentage of 88 %. Also DNA sequencing analysis and genetic relationship were conducted by phylogenetic analysis.
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George N, Bhandari V, Reddy DP. and Sharma P. (2015). Molecular and Phylogenetic analysis revealed new genotype of Theileria annulata parasites from India. Parasitology Vectors 8, 468. DOI: 10.1186/s13071-015-1075-z
Nazifi S, Razavi SM, Esmailnejad Z. and Gheisari H. (2009). Study on acute phase proteins (haptoglobin, serum amyloid A, fibrinogen, and ceruloplasmin) changes and their diagnostic values in bovine tropical theileriosis. Parasitology Research 105, 41–46. DOI: 10.1007/s00436-009-1360-x. Epub 2009 Feb 24
Demessie Y.and Derso, S. (2015). Tick Borne Hemoparasitic Diseases of Ruminants: A Review of Advance Biological Research 9(4):210-224. DOI: 10.5829/idosi.abr.2015.9.4.9516
Debabrata D, Vasundhra B, Sreenivasamurthy GS, Shweta M, Sonti R, Vandna B, Jaspreet SA, Satparkash S. and Paresh S. (2018). A
Real-Time PCR based assay for determining parasite to host ratio and parasitaemia in the clinical samples of Bovine Theileriosis. Scientific Reports 8,15441.https://doi.org/10.1038/s41598-018-33721-3
Slodki J, Jasik KP, Kepa M, Idzik D. and Wojtyczka R. (2011). Ticktransmitted disease caused by apicomplexa. Acta Protozoologica50:155–161.
Erdemir A, Aktas M. and Dumanli N. (2012). Isolation, cloning and sequence analysis of the lactate dehydrogenase
gene from Theileria annulata may lead to design of new anti Theilerial drugs. Veterinary Medicine
(10) and 559-567. DOI: 10.17221/6368-VETMED
Radostits OM, Gay CC, Hinchcliff KW. and Constable PD. (2007). Veterinary Medicine: A textbook of the diseases of cattle, horses, sheep, pigs and goats, 10th Ed. Elsevier, Philadelphia, USA,1522-1532.
Muhanguzi D, Picozzi K, Hatendorf J, Thrusfield M, Welburn SC, Kabasa, JD, Waiswa C. (2014). Prevalence and spatial distribution of Theileria parva in cattle under crop-livestock farming systems in Tororo District, Eastern Uganda. Parasites and Vectors
(91), 1–8. DOI:10.1186/1756-3305-7-91
Nourollahi-Fard SR, Khalili M. and Ghalekhani N. (2013). Detection of Theileria annulatain blood samples of native cattle by PCR and smearmethod in Southeast of Iran. Journal of Parasitic Diseases 39(2) :249–252. DOI: 10.1007/s12639-013-0333-2
Zaeemi M, Haddadzadeh H, Khazraiinia P, Kazemi B. and Bandehpour M. (2011). Identification of different Theileria species (Theileria lestoquardi, Theileria ovis, and Theileria annulata) in naturally infected sheep usingnested PCR–RFLP. Parasitology Research 108:837–843. DOI: 10.1007/s00436-010-2119-0
Ghaemi P, Hoghooghi-Rad N, Shayan P. and Eckert B. (2012). Detection of Theileria orientalis in Iran by seminested PCR.Parasitology Researcher 110:527-531. DOI: 10.1007/s00436-011-2517-y
Alkhaledi MJA. (2008). Epidemiological study of theileriosis, babesiosis and anaplasmosis in cattle of Al Qadisiya province. MSc thesis, College of Veterinary Medicine, University of Baghdad, Iraq.
Tallaf AK. (2017). Detection of Theileriasis in cattle by using polymerase chain reaction in ALMuthanna Province. MSc Thesis in
Veterinary Medicine, Baghdad University, Iraq.
ChaudhriSS. and Gupta SK. (2003). Manual of General Veterinary Parasitology. 1st Ed. Department of Veterinary Parasitology College of Veterinary Science. Haryana Agricultural University, India,pp: 46-47.
El-Naga TRA. and Barghash SM.(2016). Blood parasites in Camels (Camelus dromedarius) in Northern
West Coast of Egypt. J. of Bacteriology and parasitology 7(1): 1-7. DOI: 10.1016/j.parepi.2016.07.002
Salih DA, Hussein AM, Seitzer U. andAhmed JS.(2007). Epidemiological studies on tick-borne diseases of cattle in central equatorial state, southern Sudan. Parasitology Research 101(4):1035–1044. DOI:10.1007/s00436-007-0583-y
Muhammad G, Naureen, A, Firyal S. and Saqib M. (2008). Tick control strategies in dairy production medicine.
Pakistan Vet. J., 28(1): 43-46.
Al-Robayi HM. (1999). Epidemiology of Theileria annulata infection in Al Ashaiki farm. PhD Thesis, College of Veterinary Medicine, University of Baghdad.
Al-EmarahGY, Khudor MH. andDaham HR.(2012). Clinical, haematological and biochemical study to cattle naturally infected with
Theileria annulatain north of Basrah province. AL-Qadisiya. J. of Vet.Med.lSci. 11(1): 54-62.
Darghouth MA, Mhadhbi M, Naouach A, Boumiza A, Chaabani MF. andBen-Abderazzak S. (2010). In vivo evidence for the resistance of Theileria annulata to buparvaquone. Veterinary Parasitology 169: 241–247. https://doi.org/10.1016/j.vetpar.2010.01.013
Ziam H, Kelanamer R, Aissi M, Ababou A, Berkvens D. and Geysen D. (2015). Prevalence of bovine theileriosis in North central region of Algeria by real-time polymerase chain reaction with a note on its distribution. Tropical Animal Health and Production 47(5):787-796. DOI: 10.1007/s11250-015-0772-0
Haque M, Singh NK. and Rath SS. (2010). Prevalence of Theileria annulata infection in Hyalomma anatolicum anatolicum in Punjab state, India. Journal of Parasitic Diseases 34(1): 48-51. DOI: 10.1007/s12639-010-0004-5
Tarimo MA. (2013). Studies on the prevalence of East Coast Fever among cattle in Kilosa district. PhD thesis, Agriculture Sokoine University.
Yusufmia SB, Collins NE, Nkuna R, Troskie M, Van den Bossche P. andPenzhorna BL. (2010). Occurrence of Theileria parva and other
haemoprotozoa in cattle at the edge of Hluhluwe-iMfolozi Park, KwaZuluNatal, south Africa. J. of the South African Veterinary Assoc. 81(1): 45-49.
Gul N, Ayaz S, Gul I, Adnan M, Shams S. and Akbar N. (2015). Tropical Theileriosis and East Coast Fever in Cattle: Present, Past and Future Perspective. International J. of Current Microbiol. and Applied Sci.4(8): 1000-1018. DOI: 10.9790/2380-1105020709
Al-Saeed ATM, Omer LT, Abdo J, Habibi G, Salih D A, Seitzer U. and Ahmed J. (2010). Epidemiological studies on tropical theileriosis
(Theileria annulata infection of cattle) in Kurdistan region, Iraq Parasitol. Res
.106(2):403-404. https://doi.org/10.1007/s00436-009-1675-7
Nayel M, El-Dakhly KM, Aboulaila M, Elsify A, Hassan H, Ibrahim E. andYanai T. (2012). The use of different diagnostic tools for Babesia and Theileria parasites in cattle in Menofia, Egypt. Parasitol. Res. 111(3): 1019-1024. DOI: 10.1007/s00436-012-2926-
Epub 2012 Apr 29
Aktas M, Altay K. and Dumanli N. (2006). A molecular survey of bovine Theileria parasites among apparently cattle and with a note on the distribution of ticks in eastern Turkey. J. of Vet. Parasitology 138:179-185. https://doi.org/10.1016/j.vetpar.2006.01.052
Kohli S, Atheya UK. and Thapliyal A. (2014). Prevalence of theileriosis in cross- bred cattle: its detection through blood smear examination and polymerase chain reaction in Dehradun district, Uttarakhand, India. Veterinary World
(3): 168–171. DOI: 10.14202/vetworld.2014.168-171
Charaya G, Rakha NK, Maan S, Kumar A, Kumar T. and Jhambh, R. (2016). Comparative evaluation of polymerase chain reaction assay with microscopy for detection of asymptomatic carrier state of theileriosis in a herd of crossbred cattle. Vet. World 9(9): 1039-1040.doi: 10.14202/vetworld.2016.1039-1042