CYTOPATHOGENICITY OF INFLUENZA VIRUS (H4 N3)
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Published:
Nov 5, 2021
Keywords:
INFLUENZA VIRUS (H4 N3), chicken, CYTOPATHOGENICITY
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Abstract
Monolayer tissue cultures of chicken embryo fibroblast ( CEF ) cells infected with avian influenza virus isolate were examined by the hematoxylie and eosin (H&E) staining and indirect immunoperoxidase test for studying the cytopathogenic effect of the virus. Cytopathological changes which occurred in the uncleus of infected cells included nuclear and nucleolar hypertropy, chromatin margination and intranuclear inclusions. The most striking cytoplasmic change were the presence of perinuclear. eosinophilic inclusions at 22-36 hours post inoculation ( p. i.). Vacuolization, and granulation were also observed. Indirect immunoperoxidase ( IP ) test demonstrated the localization of influenza virus antigens in infected cells. A positive peroxidase reaction observed in the nucleus and cytoplasm were similar to those shown hematoxyline and eosin staining.
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CYTOPATHOGENICITY OF INFLUENZA VIRUS (H4 N3). (2021). The Iraqi Journal of Veterinary Medicine, 25(1), 181-193. https://doi.org/10.30539/ijvm.v25i1.1159
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How to Cite
CYTOPATHOGENICITY OF INFLUENZA VIRUS (H4 N3). (2021). The Iraqi Journal of Veterinary Medicine, 25(1), 181-193. https://doi.org/10.30539/ijvm.v25i1.1159
References
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2. Armstrong, C. L., Miranda, A. F. & Hus, K. C.(1978). Susceptibility of human skeletal muscle culture to influenza virus infection II.Cytopathology and immunofluorescence. J. Neurology Science. 35,43-57.
3. Saito, Y., Yashioka, I. & Igarashi, Y. (1970). Nuclear inclusions observed by electron microscope in cynomologus monkey kidney cells infected with
influenza virus. Virology. 40, 408-409.
4. Archetti, I.,Bereezky, E, Rosati- Valente , F and Steve- Bocciarlh, D. (1970). Elongated structures present in cells infected with influenza virus. Archiv
Virology. 29,275-286.
5. Alexander, D. J., collins, M. S. & Parkinson, M. (1982) Plaque forming ability in MDCK cells and structure of hemgglutinin of Influenza A viruses which differ in virulence for chickens. In proceedings of the first international symposium avian influenza, 22-24 April 1981. Beltsville Maryland, USA 1981, pp. 148-156.
6. Alexander, D. J. ( 1986 ) Criteria for the definition of pathogenicity of avian influenza virus. In proceeding of the second international symposium on avian influenza, 3-5 Sept. 1986. Athens: USA, pp. 228-245.
7. Bosch, F. X., Orlich, M., Klenk, H. D. &Rott, R.(1979) The structure of the hemagglutinin A determinant for the pathogenicity of influenza viruses. Virology.
95, 197-207.
8. Ibrahim, H. M., Awang, I. P. R., Alexander, D. J., Manvell, R. J., Aini, I. & Ibrahim, A. L. ( 1990 ) Isolation of influenza viruses from passerine birds in
Malaysia. Veterinary Record. 24, 582.
9. Ibrahim , H. M. (1993) In vitro and in vivo studies of Malaysian isolates of avian influenza virus. PhD thesis submitted to the Faculty of Veterinary Medicine
and Animal Science, University Pertanian, Malaysia.
10. Hsiung, G. D. ( 1973 ) Diagnostic Virology; An Illustrated Hand book. (New Haven and London: Yale University Press).
11. Russell, p. H., Griffiths, P. C. & Cannon, M.J. ( 1983) A micro well immunoperoxidase test for screening hybridomas and for diagnosis NDV and Sendai Viruses. J. Immunological Methods. 61, 156-170.
12. Rott, R.C.(1981) The role of the hemagglutinin in infectivity and pathogenicity of avian influenza viruses. In proceeding of the first international symposium on avian influenza. 22-24 April 1981. Beltsville: Maryland, USA, pp. 116- 133.
13. Anisima, E., Ghendon, Y. & Markushin, S. (1980) Ultrastructural changes in cells induced by temperature-sensitive mutants of fowl plangue virus at permissive and non- permissive temperature. J. General Virology. 47,11-16.
14. Breithenfeld, P. M. & Schafer, W. (1957) The formation of fowl plague virus antigens in infected cells studied with flourescent antibodies. Virology. 4,328-345.
15. Grossberg, S. (1963) Cytocidal assay and propagation of human influeza viruses in cultred primary hamster kidney cells. Proceedings Social Experimental Biological Medicine. 113,546-553.
16. Blaskovic. P., Rhodes, A. J., Doane, F. W. & Labzoffsky, N. A. ( 1972 ) Infection of chick embryo tracheal organ cultres with influenza A2 (Hong Kong) virus 1. Cytopathological, histopathological, immunofluorescence, hemadsorption and titration of released infections progeny. Archives Virology. 38,250-260.
17. Ciampor, F. M., Bystricka, M. & Rajcani, J. (1974) Ultrastructural localization of influenza vius antigens in infected chick embroy cells as revealed by a peroxidase-labelled antibody method Archives Virology. 46,341-352.