Comparison Study about Selected Human Infection of Zoonotic Cryptosporidiosis by Conventional Diagnostic Methods in Karbala Province, Iraq
Cryptosporidium spp Parasites were detected in man in Karbala province of Iraq by conventional methods (Flotation Methods by Sheather's sugar solution and stained with modified Ziehl-Neelsen) to study the effects of age, sex, and months on the infection rate and to record the morphological characterization of Cryptosporidium spp in patients. This study was done through the period from beginning of December 2019 to September 2020. A total of 100 fecal samples were collected from adult and young and from both sexes of human. The result recorded infection rate of Cryptosporidium spp in human in about 26%. Infection rate of Cryptosporidium spp. showed a significant relation among age groups of humans and the maximum infection rate was showed at age group 2-6 years 44% (11/25) and this percentage of infection from the number human samples at this age group, while the minimum rate was among age group 18-25 years 12% (3/25) and this percentage of infection from the number human samples at the age group. Prevalence of cryptosporidiosis in relation to the sex of infected human. The result showed no significant difference between the rate of infection and the highest rate was in males who recorded 27.41% (17/62) and this percentage of infection from the number male samples, while the lowest percentage 23.68% (9/38) was recorded in the females and this percentage of infection from the number female samples. Prevalence of Cryptosporidium spp. in human was according to Months of the year. The results of current study showed that higher infection rate with Cryptosporidium 46.66% (7/15) in February and this percentage of infection from the number of samples at this month. And the lowest rate of infection was 10% (1/10) infection rate in July and this percentage of infection from the number of samples at this month. In Conclusion, human patients indicated that the Cryptosporidium spp infection rate by using microscopic technique to be 26%, significant differences in infection rate of Cryptosporidium spp. among age groups and months of study. There was no significant difference in infection rate between sex.