Enhancing the Productive Performance of Broiler Chickens by Adding Spirulina platensis Compared with Probiotic, Prebiotics, and Oxytetracycline

  • Wisam R Atiyah College of Veterinary Medicine, University of Al-Qadisiyah, Iraq
  • Mohanad F Hamood College of Veterinary Medicine, University of Baghdad, Iraq
Keywords: Spirulina platensis, productive performance, broiler, probiotics, prebiotics


This study was conducted in order to find out the effect of Spirulina platensis (S. platensis) algae mixed with drinking water (2%) on the production performance of broiler chickens infected with or without Enterococcus faecalis (E. faecalis) and compare it with the effects of probiotics (containing Lactobacillus acidophilus, L. casi, L. reuteri, and Bacillus subtillis), prebiotics (containing antioxidants and a group of vitamins) as well as oxytetracycline. The experiment included 350 one day old (Ross-308) broiler chicks, randomly divided into 7 groups by 50 chicks per group for 35 days. The groups were divided as follows: T1: infected experimentally with E. faecalis only, T2: treated with S. platensis, T3: infected experimentally with E. faecalis and treated with 2% S. platensis, T4: infected experimentally with E. faecalis and treated with probiotic, T5: infected experimentally with E. faecalis and treated with prebiotic, T6: infected experimentally with E. faecalis and treated with oxytetracycline, and T7: control group without any addition. Weekly live body weight and weekly body weight gain were measured as well as weekly feed intake and feed conversion rate for broiler chickens were estimated. The current study proved that S. platensis algae added to drinking water (2%) in T2 and T3 groups had caused a significant increase (P<0.05) in the weekly live body weight and weekly body weight gain, Spirulina also caused a significant decrease (P<0.05) in weekly feed intake and feed conversion rate for broiler chickens. Therefore, S. platensis could be a suitable alternative to some feed additives such as probiotics, prebiotics, and antibiotics in addition to the ability of Spirulina to reduce the symptoms of E. faecalis.


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Dolka B, Go³êbiewska–Kosakowska M, Krajewski K, Kwieciñski P, Nowak T, Szubstarski J, et al. Occurrence of Enterococcus spp. in poultry in Poland based on 2014–2015 data. Med Weter. 2017;73(4): 220–4.

Gregersen R, Petersen A, Christensen H, Bisgaard M. Multilocus sequence typing of Enterococcus faecalis isolates demonstrating different lesion types in broiler breeders. Avian Pathol. 2010;39(6): 435-440.

Niewold TA. The nonantibiotic anti-inflammatory effect of antimicrobial growth promoters, the real mode of action? A hypothesis. Poult. Sci. 2007; 86: 605-609.

Zahir U R, AnwarulHaque B, Maksuda B, Md Mahfuj U P. Effect of dietary supplement of algae (Spirulina platensis) as an alternative to antibiotics on growth performance and health status of broiler chickens. In. J Poult Sci. 2019; 18: 576-584.

Noh EB, Kim YB, Seo KW, Son SH, Ha JS, Lee YJ. Antimicrobial resistance monitoring of commensal Enterococcus faecalis in broiler breeders. Poult Sci. 2020;99(5): 2675–83.

Furtula V, Jackson CR, Farrell EG, Barrett JB, Hiott LM, Chambers PA. Antimicrobial resistance in Enterococcus spp. isolated from environmental samples in an area of intensive poultry production. Int J Environ Res Public Health. 2013;10(3): 1020–36.

KimY B, SeoKwang W H S, NohEun B, LeeYoung J. Molecular characterization of antimicrobial-resistant Enterococcus faecalis and Enterococcus faecium isolated from layer parent stock. J Poult Sci. 2019;98(11):5892–5899.

Al-Shammary A. Run-off Patterns of vancomycin resistant Enterococci (VRE clones) in cows raw milk and imported milk powders at Baghdad markets. Iraqi J Vet Med. 2019;43(2): 65-70. Available from: http://jcovm.uobaghdad.edu.iq/index.php/Iraqijvm/article/view/532

Han D, Unno T, Jang J, Lim K, Lee S N , Ko G, et al. The occurrence of virulence traits among high-level aminoglycosides resistant Enterococcus isolates obtained from feces of humans, animals, and birds in South Korea. Int. J. Food Microbiol.2011; 144:387-392.

O'Driscoll T, Crank CW. Vancomycin-resistant enterococcal infections: epidemiology, clinical manifestations, and optimal management. Infect Drug Resist. 2015; 24:217-230. doi:10.2147/IDR.S54125

Fiore E, Van Tyne D, Gilmore MS. Pathogenicity of Enterococci. Microbiol Spectr.2019;7(4): 10.1128/microbiolspec.GPP3-0053-2018. doi:10.1128/microbiolspec. GPP3-0053-2018.

Kiczorowska B, Al-Yasiry A, Samoliñska W, Marek A, Pyzik E. The effect of dietary supplementation of the broiler chicken diet with Boswellia serrata resin on growth performance, digestibility and gastrointestinal characteristics, morphology and microbiota. Livest. Sci.2016; 191: 117-124.

Sugiharto S, Yudiarti T, Isroli I, Widiastuti E, Putra FD. Intestinal microbial ecology and hematological parameters of broiler fed

cassava waste pulp fermented with Acremonium charticola. Vet. World. 2017; 10: 324-330.

Soni RA, Sudhakar K, Rana R. Spirulina - from growth to nutritional product: a review. Trends Food Sci. Technol. 2017; 69: 157-171.

Mirzaie S, Zirak-Khattab F, Hosseini SA, Donyaei-Darian H. Effects of dietary Spirulina on antioxidant status, lipid profile, immune response and performance characteristics of broiler chickens reared under high ambient temperature. Asian-Australas J Anim Sci.2018;31(4): 556-563. doi:10.5713/ajas.17.0483.

Pestana JM, Puerta B, Santos H, Madeira MS, Alfaia CM, Lopes PA, et al. Impact of dietary incorporation of Spirulina (Arthrospira platensis) and exogenous enzymes on broiler performance, carcass traits, and meat quality. Poult Sci. 2020;99(5):2519–32.

Zhao P, Wu G, Zhang Q, Chu J, Xie C, Wang Y, et al. Experimental investigation on Ornithobacterium rhinotracheale and Enterococcus faecalis co-infection in chickens. Pak Vet J. 2015;35(2):173–177.

Mendes AS, Paixao SJ, Restelatto R, Reffatti R, Possenti JC, de Moura DJ,et al . Effects of initial body weight and litter material on broiler production. Rev Bras Cienc Avic. 2011; 13: 165-170. DOI: 10.1590/S1516-635X2011000300001

Shroha A, Bidhan DS, Sihag SS, Yadav DC. Body weight, feed consumption and FCR of broiler chicken upon dietary Supplementation of Ajwain (Trachyspermum ammi). Int. J. Curr. Microbiol. App. Sci. 2019;8(2):2189-98.

Mahmood M, Ahmad M, Hussain I, Abbas R, Khan A, Rafiq A. Growth promoting effect of Pimpinella anisum (Aniseed) in broiler chickens. Bol Latinoam Caribe Plantas Med Aromat.2014; 13. 278-284.

Landau S, Everitt BS. A handbook of statistical analyses using SPSS [Internet]. London, England: Chapman and Hall; 2003. Available from: http://dx.doi.org/10.1201/9780203009765.

Grajek W, Olejnik A, Sip A. Probiotics, prebiotics, and antioxidants as functional foods. Acta Biochim Pol. 2005;52(3):665-671.

Zeweil H, Abaza I, Zahran S, Ahmed M, Haiam M, Aboul-Ela, et al. Effect of Spirulina platensis as dietary supplement on some biological traits for chickens under heat stress condition. Asian j. biomed. pharm. sci.2016; 6(56): 08-12.

Jung F, Krüger-G A, Waldeck P, Küpper J-H. Spirulina platensis, a super food? J Cell Biotechnol.,2019; 5: 43-54. 10.3233/JCB-189012.

Abu-Elala NM, Galal MK, Abd-Elsalam RM, Mohey-Elsaeed O, Ragaa NM. Effects of dietary supplementation of Spirulina platensis and garlic on the growth performance and expression levels of immune-related genes in Nile tilapia (Oreochromis niloticus). J Aquac Res Dev.2016; 7:433. doi:10.4172/2155-9546.1000433.

Hajati H, Zaghari M. Effects of Spirulina platensis on growth performance, carcass characteristics, egg traits and immunity response of Japanese quails. Iran J Appl Anim Sci, 2019; 9(2): 347-357.

Mariey YA, Samak MA. Effect of using Spirulina platensis algae as a feed additive for poultry diets: 1- productive and reproductive performances of local laying hens. Egypt Poult Sci. 2012;32 (I):201–215.

Tavernari F C, Roza L F, Surek D, Sordi C, Silva M, Albino L, et al. Apparent metabolisable energy and amino acid digestibility of microalgae Spirulina platensis as an ingredient in broiler chicken diets. Br Poult Sci. 2018; 59(5): 562–567. https://doi.org/10.1080/00071668.2018.1496401

Sharmin F, Sarker NR, Sarker MSK. Effect of using Moringaoleifera and Spirulina platensis as feed additives on performance, meat composition and oxidative stability and fatty acid profiles in broiler chicken. Nutr. Food Sci. 2020; 10: 772. doi: 10.35248/2155-9600.20.10.772.

Jose AM, Diana G, Martínez F, Javier R, Pedro J. Enrichment of vitamin E from Spirulina platensis microalga by SF supercrit fluids. 2008; 43 (3): 484-489.

Hasan Z, Ibrahim M A, Soliman M, Mohamed H, Haiam M, Asmaa A. Effect of Spirulina platensis as dietary Supplement on some biological traits for chickens under heat stress condition. Asian j. biomed. pharm. sci.2016; 6(56): 08-12.

Kaoud HA. Effect of Spirulina platensis as a dietary supplement on broiler performance in comparison with prebiotics. Sci. J. App. Res. 2012;1 (2): 44-48.

How to Cite
Atiyah, W., & Hamood, M. (2021). Enhancing the Productive Performance of Broiler Chickens by Adding Spirulina platensis Compared with Probiotic, Prebiotics, and Oxytetracycline. The Iraqi Journal of Veterinary Medicine, 45(1), 31-36. https://doi.org/10.30539/ijvm.v45i1.1037