Study of important moulds isolated from milk and its products in Baghdad – with experimental study the pathogenesis of Aspergillus Fumigatus moulds in mice Bushra.I.Al-Kaisie , Jinan Khalid

Abstract

This study was designed to isolate and identify the type of moulds and their
percentage of distribution with pathological study of highly moulds isolated from
(130)samples were included in this study with divided into (30)samples dried
milk,(30).samples baby food ,(30)samples raw milk , (40) samples dairy products .
The results revealed the percentage of infection according to total count of
isolation in raw milk were 100 % dairy product 65 %. The most common fungi
isolated from raw milk were Asp.niger of 16.2 % Asp. fumigatus of 15 % Asp.flavus
of 13.7 % Pencillium spp. 12.5 % . From dairy products were isolated with
percentage of Asp. Fumigatus 34.9% Geotrichum spp . of 23 % ,Asp. nager of 11.5
% . Pathological study was carried out to investigate the clinical signs with grossly
and Histopathological changes inoculated with most pathogenic moulds Asp.
fumigatus (30)male white swiss mice were used in this study and divided into four
groups . The first group were injected I/P with 0.2 ml of Asp. fumigatus containing
1X105 spores / ml , 4th group (control)given orally 0.2 ml of PBs.Two mice from
each treated groups and one from each control group were scarified on week (1,2,3,4)
.the clinical manifestation of the 1st group were at 2nd week showed dyspnea .Mould
isolated from blood and internal organs at third week , with no isolation from 3rd
group. The Histopathological observations showed presence of degeneration ,
necrosis and diffused granuloma in liver, lung , spleen , kidney and hemorrhage with
thrombosis in brain and intestine .