Preparation of Local Diagnostic Kit for Salmonella typhimurium Zainab Sami Habeeb, Amal Majid Al-Shawi, Muniem M. fathi

Abstract

Some of somatic and flageller antgens from both the human and animal isolates were prepared,
and were used to Immunize a group of rabbits by intravenous injections at an upgrading doses.The
results of this immunization showed the gaining of an excellent hyperimmune serum. With the use
of absorptions technique amonospecific antiserum was obtained for the specific injected antigenes, the
standardization of the antibodies showed a highly immune response reading. 1: 2560 , 1: 5120 to H:I
and O: 4.5 Antigens respectively for both antisera.
This study showed the report for the 1st time the preparation for diagnostic kit for somatic and flagellar
antigens to S. typhimurium comparing this method to Widal test with a good results and Response