Effect of Sperm Selection by ''Swim-Up" Technique on the Sex Ratio of In Vitro Produced Ovine Embryos Saja Hadi and Ihsan H. AL-Timimi

Abstract

      The objective of this study was to investigate the percentage of male and female ovine embryos produced after oocytes fertilization by sperm selected with self-migration (swim-up) technique. The sex of in vitro produced embryos was determined by Polymerase chain reaction (PCR). results indicate that the fertilization by sperm selected using Swim-up technique was 81.8%  (9/11) male and 18.1% (2/11) female in vitro produced embryos when centrifuged at 200×g, while centrifugation at 300×g  was 72.7% (8/11) male and 27.2% (3/11) female embryos. It was concluded that the use of “swim-up” technique refer to significant (P<0.05) occurred for increasing male embryos ratio compared with normal ratio (50%) which represent the sex, and it is for the first time, preselected sperms used for In Vitro fertilization (gender selection) in farm animals in Iraq.