Study of some virulence factors of Aeromonas Spp. isolated from stool samples of children with diarrhea

Main Article Content

Saba Talib Hashim
Mahmoud Mohammed Nema

Abstract

     Three hundred samples of stool were collected from children, who are suffering from diarrhea, during the period from October to December 2016 at Fatima Al Zahra and Shaheed al-Sadr hospitals. All bacterial isolates have been submitted to the cultural, microscopical, biochemical examinations, and Vitek 2 system identification to identify Aeromonas isolates. All the isolates under study were tested for their ability to the production of certain factors associated with virulence. It has been concluded that 12 (4%) of collected samples were positive to Aeromonas Spp. The virulence factors of Aeromonas spp. were detected and showed that all the isolates have the ability to produce haemolysin, protease, lipase, phospholipase, gelatinase enzymes, and biofilm on Congo red agar.

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Study of some virulence factors of Aeromonas Spp. isolated from stool samples of children with diarrhea. (2018). The Iraqi Journal of Veterinary Medicine, 42(1), 23-27. https://doi.org/10.30539/iraqijvm.v42i1.26
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How to Cite

Study of some virulence factors of Aeromonas Spp. isolated from stool samples of children with diarrhea. (2018). The Iraqi Journal of Veterinary Medicine, 42(1), 23-27. https://doi.org/10.30539/iraqijvm.v42i1.26

References

Subashkumar, T.R.; Thaumanvan, S.T.; Vivekandhan, S.G. and Lakshman, T.P. (2016). Occurrence of Aeromonas hydrophila in acute gastroenteritis among children. J. Med. Res., 123(l):6-61.

Nahharo, N.Q. (2014). Bacteriological and immunological study of gram-positive bacteria and mechanisms of their targeting of the cell envelope. Microbiol. Mol. Biol. Rev., 63:174-229. https://doi.org/10.1128/MMBR.63.1.174-229.1999

Meiyanti, S.; Salim, O.C.; Surjawidjaja, J.E. and Lesmana, M. (2010). Isolation and antibiotic sensitivity of Aeromonas Spp. from children with diarrhea. Uni. Med., 29(1):1420.

Uche, O.A. and Kennedy, N.J. (2014). Prevalence of Aeromoas Spp among patients attending general hospital. Advanced Medical Sciences: Int. J., 1:1-10.

Galindo, C.L. and Chopra, A.K. (2007). Aeromonas and Plesiomonas Spp in food microbiology, fundamentals and frontiers. ASM Press., 6:381-401. https://doi.org/10.1128/9781555815912.ch17

Chopra, A.K.; Xu, X.J.; Ribardo, D.; Gonzalez, M.; Kuhl, K.; Peterson, J.W. and Houston, C.W. (2000). The cytotoxic enterotoxin of Aeromonas hydrophila induces pro inflammatory cytokine production and activates arachidonic acid metabolism in macrophages. Infect. Immun., 68:2808-2818. https://doi.org/10.1128/IAI.68.5.2808-2818.2000

Janda, J.M. and Abbott, S.L. (2010). The gnus Aeromonas, taxonomy, pathogenicity, and infection. Clin. Microbiol. Rev., 23:3573.

https://doi.org/10.1128/CMR.00039-09

MacCFadin, J.K. (2000). Biochemical Tests for Identification of Medical Bacteria. 3rd Ed. Philadelphia Baltimore, NewYork. Lippincott Williams and Winkins. Walter Klumer Company, Pp:25-40.

Jawetz, E.; Melnick, J.I. and Adelberg, E.A. (2007). Medical Microbiology. 24th Ed. USA. Appleton and Lange Books, Pp:55.

Collee, J.G.; Fraser, A.G.; Marmino, B.P. and Simons, A. (1996). Mackin and McCartney Practical Medical Microbiology. 14th Ed. USA. The Churchill Livingstone, Inc., Pp:55.

Benson, H.J. (2002). Microbiological Applications: Laboratory Manual in General Microbiology. 8th Ed. USA. McGraw-Hill, Pp:53.

Lobna, A.J. (2016). Physiological study for extracted surface layer from Aeromonas hydrophila isolated from diarrhea. Mesopotamia Environ. J., 2(3):11-26.

Obaid, A.J. (2013). Effect of some physiological factor on s-layer synthesis of Aeromonas hydrophila in Babylon province. M.Sc. Thesis, Collage of Science, Babylon University, Pp:125-130. (Arabic).

Rogo, L.D.; Attah, A.; Bawa, E.; Aliyu, A.M.; Aliyu, M.S. and Gaiya, A.Z. (2009). Antimicrobial susceptibility Aeromoas hydrophila pattern among of patients presented with diarrheal attending two teaching hospitals in Northern Nigeria. Bayero J. Pure Appl. Sci., 2(1):91-95.

https://doi.org/10.4314/bajopas.v2i1.58475

Guerra, I.M. (2007). Aeromonas associated diarrheal disease in South Brazil. Brazilian J. Microbiol., 38:638-643.

https://doi.org/10.1590/S1517-83822007000400011

Brenner, D.J.; Krieg, N.R. and Staley, J.S. (2005). Bergey's Manual of Systematic Bacteriology. Vol. 2, 2nd Ed. New York. Springer, Pp:556-578. https://doi.org/10.1007/0-387-28021-9

Samal, S.K.; Das, B.K. and Pal, B.B. (2014). Isolation, biochemical characterization, and antibiotic susceptibility study of Aeromonas hydrophila isolated from freshwater fish. Int. J. Current Microbiol. Appl. Sci., 3(12):259267.

Winn, W.C.; Allen, S.D.; Janda, W.M.; Koneman, E.W.; Procop, G.W.; Schreckenberger, P.C. and Woods, G.L. (2006). Koneman's Color Atlas and Text Book of Diagnostic Microbiology. 6th Ed. USA. Lippincott Williams and Wilkins, Pp:422-440.

Kivanc, M.; Yilmaz, M. and Demir, F. (2011). The occurrence of Aeromonas in drinking water and the Porsuk river. Brazilian J. Microbiol., 42:126-131. https://doi.org/10.1590/S1517-83822011000100016

Dooley, J.S.; Lallief, R. and Trust, T.J. (2014). Surface antigens of virulent strains of Aeromonas hydrophila. Vet. Immunol. Immunopathol., 12:339-344. https://doi.org/10.1016/0165-2427(86)90138-8

Taj Aldeen, W.R.; Al-Rubaiae, A.F.; AlBermani, O.K. and Naj, S.N. (2014). PCR detection of putative haemolysin and erolysin genes in Aeromonas hydrophila isolates from diarrhea in Babylon province. Ph.D. Thesis. Collage of Science, Babylon University, Pp:120-125.

Anju, P.; Melinda, N. and Santosh, K.D. (2010). Hemolysin, protease, and EPS producing pathogenic Aeromonas hydrophila strain An4 shows antibacterial activity against marine bacterial fish pathogens. J. Marine Biol., 12:1-9. https://doi.org/10.1155/2010/563205

Hamdi, R.; Khan, M.A.; Ahmad, M.; Ahmad, M.M.; Abdin, M.Z.; Mussarat, J. and Javed, S. (2013). Chitinases: An update. J. Pharma Sci., 5(1):21-23. https://doi.org/10.4103/0975-7406.106559

Abd-Elmalek, A.M. (2017). Incidence and virulence characteristics of Aeromonas spp. in fish. Veterinary World, 10(1):34-37.

https://doi.org/10.14202/vetworld.2017.34-37

Kadhim, S.A. (2009). Detection of some bacterial causes of watery diarrhea in the province of Baghdad and some northern governorates, with a study of pathological effects. M.Sc. Thesis, College of Science, University of Baghdad, Pp:125-130. (Arabic).

Troy, S.; Jasmine, S.; Alexander, A.;Christopher, G.; and Greg, A. (2014). Aeromonas hydrophila and Aeromonas veronii predominate among potentially pathogenic ciprofloxacin- and tetracyclineresistant Aeromonas isolates from Lake Erie. Appl. Environ. Microbiol., 80(3):841-848. https://doi.org/10.1128/AEM.03645-13

Tiianchezian, S.; Jayaraman, S.; Manoharam, S. and Valsalon, S. (2010). Virulence and cytotoxicity of seafood borne A. hydrophila. Brazilian J. Microbiol., 41(4):978 -983. https://doi.org/10.1590/S1517-83822010000400016

Kirov, S.M.; Castrisio, M. and Shaw, J.G. (2004). Aeromonas flagella (polar and lateral) are enterocyte adhesions that contribute to biofilm formation on surface. Infec. Immun., 72:1939-1945. https://doi.org/10.1128/IAI.72.4.1939-1945.2004

Sinha, M.; Simoes, L. and Vieira, M. (2010). A review of current and emergent biofilm control of Aeromonas Spp. strategies. LWT - Food Sci. Technol., 43:573-583. https://doi.org/10.1016/j.lwt.2009.12.008

Soaris, C. (2008). Characterization of Aeromonas Spp. isolates from newborns hospitalized. Pereira C.S. Cols., 41(2):179182.

Dogan, B. and Boor, K.J. (2003). Genetic diversity and spoilage potentials among Aeromonas Spp. isolated from fluid milk products and dairy processing plants. Appl. Environ. Microbiol., 69(1):130- 138. https://doi.org/10.1128/AEM.69.1.130-138.2003

Thornley, J.P.; Shaw, J.G.; Gryllos, I.A. and Eley, A. (1997). Virulence properties of clinically significant Aeromonas species evidence for pathogenicity. Rev. Med. Microbiol, 8:61-72. https://doi.org/10.1097/00013542-199704000-00002

Yang, L.; Li, L. and Qun, J. (2011). Characterization of virulence properties of Aeromonas veronii isolated from diseased Gibel Carp (Carassius gibelio). Int. J. Moi. Sci., 17(4):1-11.

Vila, J.; Ruiz, J.; Gallardo, F.; Soler, L.; Figueras, M. J. (2003). Aeromonas Spp. and traveler's diarrhea: Clinical features and antimicrobial resistance. Emerg. Infect. Dis., 9:552-555. https://doi.org/10.3201/eid0905.020451

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