The Protective role of Pomegranate seed oil (Pometon) on kidney (functional and structural) damage induced by Methionine overload in adult female Rabbits

Abstract

     This study  was designed to investigate the protective effect of Pomegranate seed oil (PSO) on kidney damage induced by methionine overload in adult female rabbits. Twenty four  adult female local rabbits were randomly divided into four equal groups, and they were treated daily for 42 days,as fallow: the first group (C): rabbits  were received ordinary corn oil orally, serving as control, while aniamls in the second  group T₁ were intubated with methionine (100 mg/kg BW) orally and animals in third group (T₂)  were intubated with methionine (100mg/kg BW) plus pometon (30mg/kg BW) diluted in corn oil wihle, rabbits in fourth group (T₃) were intubated with pometone (30 mg/kg BW) diluted in corn oil.Fasting blood samples were collected from all experimental groups at 0, 21, and 42 days of experiment to study the parameters: Serum creatinine (SC) concentration and Blood urea nitrogen (BUN) concentration. Sections of kidney were assessed for histopathological studies. The result revealed that exposure of animals to methionine in group (T₁) for 42 days caused a significant increase (P<0.05) in SC, BUN, concentrations as comparing to pomegranate  treated groups and control. While oral intubation of PSO caused significant decrease (P<0.05) in the previous and correct the values near that of the control. Exposure to methionine overload showed severe acute cellular degeneration with   mononuclear cells infiltration between renal tubules while  there was no clear pathlogical lesions in kidney section after PSO intubation. In conclusion, it seems that  Pomegranate seed oil intubation possessed renoprotactive (functional and structural) effect against deleterious damage induced by Methionine overload  in rabbits.