Differentiated Stem Cells Derived from Rabbit Adipose Tissue Exhibited in Vitro Adipogenesis and Osteogenesis
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Abstract
The multipotent characteristic of rabbit adipose-derived stem cells makes them available and convenient sources for isolating mesenchymal stem cells. The aim of this study was to assess the differentiation in rabbit adipose-derived stem cells pre-committed to produce several mesenchymal lineages in response to inductive extracellular cues to multipotent stromal cells. Three grams of adipose tissue was taken from a subcutaneous region of the nape of the neck and was carefully isolated to obtain mesenchymal stem cells for expanded by fourth passage. In the 4th passage, active growth of mesenchymal stem cells was observed. Furthermore, the research demonstrated the inherent ability of rabbit MSCs to induce differentiation in osteogenic and adipogenic lineages. These mesenchymal stem cells were successfully isolated from adipose tissue which differentiated into either osteocytes or adipocyte-like cells after 21 and 14 days of culturing in specific osteogenic and adipogenic media, respectively. The remarkable differentiation potential of rabbit mesenchymal stem cells is indicated by mineralized deposition to the osteocytes and lipid droplets accumulated in the cytoplasm lipid vacuoles in the adipocytes.
Received: 31 July 2023
Revised: 17 August 2023
Accepted: 15 October 2023
Published: 28 December 2023
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