Effect of Cryopreservation on Sperms Function Parameters and In vitro Fertilization Rate in Mice
Article Sidebar
Main Article Content
Abstract
The study was carried out to investigate the effect of cryopreservation in liquid nitrogen (-
196ºC) on sperm quality (concentration, motility, morphology) and in vitro fertilization rate (IVF),
24 hours post insemination in mice. The sperms were obtained from caudal epididymis of 20 mature
male mice and prepared by Direct Activation Technique after cryopreservation followed by
insemination the oocyte of 20 female mice using in vitro fertilization procedure. Results showed
that there were significant (P > 0.05) decrease in sperms concentration, motility and morphology post
cryopreservation. In addition, the results demonstrated that cryopreservation significantly decreased
(P > 0.05) fertilization rate in treated mice (36%) compared to control mice (56%). It is conclusion
that frozen sperm can be used without compromising pregnancy chances.
Downloads
Article Details
How to Cite
References
Abdelhafez, F.; Bedaiwy, M.; El-Nashar, S.A.; Sabanegh, E. and Desai, N. (2009). Techniques for cryopreservation of
individual or small numbers of human spermatozoa: a systematic review. Human Reproduction Update, 15:153–164.
Behrman, S.J. and Sawada, Y. (1966). Heterologous and homologous inseminations with human semen frozen
and stored in a liquid-nitrogen refrigerator. Fertility and Sterility, 17(4):457–466.
Anger, J.T.; Gilbert, B.R. and Goldstein, M. (2003). Cryopreservation of sperm: indications, methods and results. J.
Urology, 170(41):1079–1084.
Sanger, W.G.; Olson, J.H. and Sherman, J.K. (1992). Semen cryobanking for men with cancer—criteria change. Fertility and
Sterility, 58(5):1024–1027.
Medeiro, C.M.; Forell, F.; Oliveira, A.T. and Rodrigues, J.L. (2002). Current Status of sperm cryopreservation: why isn’t it
better. Theriogenology, 57:327-344.
Lucena, E. and Obando, H. (1986). Comparative analysis of different glycerol levels when used as cryoprotective agent
on human spermatozoa. P:553. In: Paulson, J.D. (ed) Andrology: male fertility and sterility. Academic Press, NewYork, USA.
Cha, K.Y.; Han, S.Y. and Chung, H.M. (2000). Pregnancies and deliveries after In vitro maturation culture followed by In
vitro fertilization and embryo transfer without stimulation in women with polycystic ovary syndrome. Fertility and
Sterility, 73:978-983.
Sherman, J. (1990). Cryopreservation of human semen. PP:68-71 In: Handbook of the Laboratory Diagnosis and Treatment
of Infertility. CRC Press. Boca Raton, Fla, USA.
Mortimer, D. (1994). Semen cryopreservation. In: Mortimer, D. (ed). Practical laboratory andrology. Oxford
University Press, Oxford, PP:301-323.
Nagy, A.; Gertsenstein, M.;Vintersten, K. and Behringer, R. (2003). PP:31-61, In: Manipulating the Mouse Embryo, A
Laboratory Manual. 3rd ed. Cold Springer Harbor Press. New York, USA.
Cross, N.L. and Overstreet, J.W. (1987).Glycoconjugates of the human sperm surface: distribution and alterations that
accompany capacitation in vitro. Gamete Res., 16(1):23–35.
Jensen, J.R. and Morbeck, D.E. (2011). Fertility preservation. Mayo Clinic Proceedings, 86(1):45–49.
Watson, P.F. (2000). The causes of reduced fertility with cryopreserved semen. Ani. Reprod. Sci., 60-61:481–492.
Thachil, J.V. and Jewett, M.A. (1981). Preservation techniques for human semen. Fertility and Sterility, 35(5):546–548.
Taylor, K.; Roberts, P.; Sanders K. and Burton P. (2009). Effect of antioxidant supplementation of cryopreservation
medium on post-thaw integrity of human spermatozoa. Reproductive BioMedicine Online, 18(2):184–189.
Ford, W.C. and Rees, J.M. (1990). Thebioenergetics of mammalian sperm motility. Biological and Clinical Aspects.
Boca Raton. Fla. USA. CRC Press. pp. 175–202.
O’Connell, M.; McClure, N. and Lewis, S.E. (2002). The effects of cryopreservation on sperm morphology,
motility and mitochondrial function. Human Reproduction. 17(3):704–709.
Mahadevan, M. and Trounson, A.O. (1984). Effect of cooling, freezing and thawing rates and storage conditions on
preservation of human spermatozoa. Andrologia, 16(1):52–60.
Zamboni, L. (1987). The ultrastructural pathology of the spermatozoon as a cause of infertility: the role of electron
microscopy in the evaluation of semen quality. Fertility and Sterility, 48(5):711–734.
Lasso, J.L.; Noiles, E.E.; Alvarez, J.G. and Storey, B.T.(1994). Mechanism of superoxide dismutase loss from human
sperm cells during cryopreservation. J. Andrology, 15(3):255–265.
McClintock, D.S.; Santore, M.T. and Lee, V.Y. (2002). Bc1-2 family members and functional electron transport chain
regulate oxygen deprivation-induced cell death. Molecular and Cellular Biology, 22(1):94–104.
Martin, G.; Cagnon, N. and Sabido, O. (2007). Kinetics of occurrence of some Features of apoptosis during the cryopreservation process of bovine spermatozoa. Human Reprod., 22(2):380–388.
Martinez-Soto, J.C.; De Dioshourcade, J.; Gutiérrez-Adán, A.; Landeras, J.L. and Gadea, J. (2010). Effect of genistein
supplementation of thawing medium on characteristics of frozen human spermatozoa. Asian J. Andrology, 12(3):431–441.
Branco, C.S.; Garcez, M.E.; Pasqualotto, F.F.; Erdtman, B. and Salvador, M.(2010). Resveratrol and ascorbic acid
prevent DNA damage induced by cryopreservation in human semen. Cryobiology, 60(2):235–237.
Li, Z.; Lin, Q.; Liu, R.; Xiao, W. and Liu, W. (2010). Protective effects of ascorbateand catalase on human spermatozoa
during cryopreservation. J. Andrology, 31(5):437–444.
Stanic, P.; Tandara, M.; Sonicki, Z.;Simunic, V.; Radakovic, B. and Suchanek, E. (2000). Comparison of protective
media and freezing techniques for cryopreservation of human semen. European J. Obst. Gynecol. and Reprod.
Biol., 91(1):65–70.
Said, T.M.; Gaglani, A. and Agarwal, A. (2010). Implication of apoptosis in sperm cryoinjury. Reprod. BioMed. Online, 21(4):456–462.
Mazur, P.; Rall, W.F. and Rigopoulos, N. (1981). Relative contributions of the fraction of unfrozen water and of salt
concentration to the survival of slowly frozen human erythrocytes. Biophysical J., 36(3):653–675.
Arav, A.; Zeron, Y.; Leslie, S.B.;Behboodi, E.; Anderson, G.B. and Crowe, J.H. (1996). Phase transition temperature
and chilling sensitivity of bovine oocytes. Cryobiology, 33(6):589–599.
Zeron, Y.; Pearl, M.; Borochov, A. and Arav, A. (1999). Kinetic and temporal factors influence chilling injury to
germinal vesicle and mature bovine oocytes. Cryobiology, 38(1):35–42.
Giraud, M.N.; Motta, C.; Boucher, D. and Grizard, G. (2000). Membrane fluidity predicts the outcome of cryopreservation
of human spermatozoa. Human Reprod.,15(10):2160–2164.
Quinn, P.J. (1985). A lipid-phase separation model of low-temperature damage to biological membranes.
Cryobiology, 22(2):128–146.
Talaei, T.; Esmaeelpour, T.; Aekiyash, F. and Bahmanpour, S. (2010). Effects of cryopreservation on plasma membrane
glycoconjugates of human spermatozoa. Iranian J. Repro. Med., 8(3):119–124.
Benoff, S. (1997) Carbohydrates and fertilization: an overview. Molecular Human Reprod., 3(7):599–637.
Lassalle, B. and Testart, J. (1994). Human zona pellucida recognition associated with removal of sialic acid from human sperm
surface. J. Reprod. and Fertility, 101(3):703–711.