The protective effect of the aqueous extract of parsley (Petroselinum sativum ) seeds on experimentally – induced oxidative stress in rats
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Published:
Nov 5, 2021
Keywords:
rats, aqueous extract of parsley, Petroselinum sativum
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Abstract
In this study, the potential protective effect of aqueous extract of parsley (Petroselinum sativum) seeds against hydrogen peroxide (H2O2) – induced oxidative stress in male rats was assessed. Three groups of male albino rats were randomly divided (n=7) and were handled for twenty-eight days as follows: rats in group I served as control; animals in group || were provided with drinking water containing 0.5% H2O2 and those in group III received orally 8 mg/100 gm B.W. of aqueous extract of parsley seeds plus 0.5% H2O2 in drinking water. After four weeks experimental period, a significant increase in lipid peroxidation products (MDA), and decrease in glutathione (GSH) concentrations were observed in plasma, kidney, liver and heart tissues of H2O2 treated animals as compared with the control group. These biomarkers (GSH and MDA) are interrelated and indicate the occurrence of oxidative stress. Plasma total cholesterol (TC) concentration was significantly increased in H2O2 treated rats. By administration of aqueous extract of parsley along with H2O2, plasma and tissue GSH levels were significantly increased while the elevation in MDA level was diminished in plasma and different tissues examined. A decrease in plasma cholesterol concentration was recorded in H2O2 and parsley treated group as compared with the control one and H2O2 treated groups. These results indicate that aqueous extract of parsley have hypocholesterolemic and antioxidant effect.
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The protective effect of the aqueous extract of parsley (Petroselinum sativum ) seeds on experimentally – induced oxidative stress in rats. (2021). The Iraqi Journal of Veterinary Medicine, 25(1), 154-172. https://doi.org/10.30539/ijvm.v25i1.1157
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How to Cite
The protective effect of the aqueous extract of parsley (Petroselinum sativum ) seeds on experimentally – induced oxidative stress in rats. (2021). The Iraqi Journal of Veterinary Medicine, 25(1), 154-172. https://doi.org/10.30539/ijvm.v25i1.1157
References
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challenge with H2O2. Msc. Thesis, College of Pharmacy. University of Baghdad.
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24. Hennig, B.; Toborek, M.; McClain, C.J. and Diana, J.N. (1996). Nutritional implications in vascular endothelial cell metabolism. J Am College of Nutr.,
15(4): 345-358.
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26 . Taniguchi, M.; Yasutake, A. and Takedomi, K. (2000). Effects of dietary sulfur-containing amino acids on oxidative damage in rat liver caused by N-nitrosodimethylamine administration. British J Nutr., 84: 211-217.
27 · van Acker, S.A.; van den Berg, D.J.; Tromp, M.N.; Griffioen, D.H. and Bast, A. (1996). Structural aspects of antioxidant activity of falvonoids. Free Radic Biol Med., 20: 331 342.
28. Hempel, J.; Pforte, H.; Raab, B.; Engst, W.; Bohm, H. and Jacobasch, G. (1999). Flavonols and flavones of parsley cell suspension culture change the antioxidative capacity of plasma in rats. Nahrung, 43(3): 201-4. (Midline Abstract).
29. Kreeman, V.; Skottova, N.; Walterova, D.; Ulrichora, J. and Simanek, V. (1998). Silymarin inhibits the development of hypercholesterolemia in rats. Planta diet induced Medica., 64: 138-142.
2. Halliwell, B. and Gutteridge, J.M.C. (1984). Lipid peroxidation, oxygen radicals, cell damage and antioxidant therapy. Lancet., 1: 1396-1397.
3. Visioli, F. and Galli, C. (1997). Evaluating oxidation processes in relation to cardiovascular disease: a current review of oxidant / antioxidant methodology. Nutr Metab Cardiovasc Dis.,7: 459-466.
4. Diguiseppi, J. and Fridovich, I. (1983). The toxicology of molecular oxygen, CRC Critical Review in Toxicology., 12: 315-342.
5. Bernnan, L.A.; Morris, G.M.; Wasson, G.R. and Hnnigan, B.M. (2000). The effect of vitamin C or vitamin E supplementation on basal and H2O2-induced DNA damage in human lymphocytes. British J Nutr., 82: 23-30.
6· Southrn, P.A. and Powis, G. (1988). Free radicals in medicine. Chemical nature and biological reactions. Mayo Clin Proc., 63: 381-389.
7. Kritchevsky, S.B. (1999). B-carotene, carotenoids and the prevention of coronary heart disease. J Nutr., 129(1): S8.
8. Khudiar, K.K. (2000). The role of aqueous extracts of olive
(Olea europaea) leaves and garlic (Allium sativum) in ameliorating the effects of experimentally induced atherosclerosis in rats. Ph.D. Thesis, College of Veterinary
Medicine, University of Baghdad.
9· Azize, B.N. (1999). Certain biochemical changes associated with starvation, oxidative stress and experimental diabetes mellitus in rats: Effects of some medical plants and female sex hormones. Ph.D. Thesis, College of Veterinary Medicine. University of Mousl.
10 . Nielson, S.E. and Dragsted, L.O. (1998). Column switching high-performance liquid chromatographic assay for determination of apigenin and acacetin in human urine with ultraviolet absorbance detection. J Chematogr Biomed Sci Appl., 713(2): 379-86.
11. Chakravarty, H.L. (1976). Plant Wealth of Iraq: A Dictionary of Economic Plants. Vol. I, Ministry of Agriculture and Agrarian Reform. Baghdad Pp:
12 . AL-Okaily, B.N. (1999). Effect of parsley seeds extract (Petroselinum sativum) on kidney functions in rats. Ph.D. Thesis, College of Veterinary Medicine. University of Baghdad.
13 . Al-Zubaidi, Z.N.; Baban, H.A. and Flayeh, F.K. (1996). A theraputic Index of Iraqi Medicinal Plant. P: 107. A'AB printing Co., Baghdad, Iraq.
14 . Low, P.A.; Nickader, K.K. and Tritschler, H.I. (1977). The roles of oxidative stress and antioxidant treatment in experimental diabetic neuropathy. Diabetes., 46, supp.2: S38-542.
· 15 · Ellman, G.L. (1959). Tissue sulfhydryl groups. Arch Biochem Biophys. 82: 70-77.
16 . Gilbert, H.S.; Stump, D.D. and Roth, E.F. (1984). A method of correct for errors caused by generation of interfering compounds during erythrocyte lipid peroxidation. Analyt Biochem., 137: 282-286.
17 . Harborne, J.B.; Mabray, T.J. and Mabray, H. (1975). Physiology of function of flavonoids, P: 970-1042. The Favoids Acad. Press. New York, San Francisco.
18. Snedecor, G.W. and Cochran, W.G. (1973). Statistical Methods. 6th ed. The lowa state university press. Pp: 238-248.
19. Al-Kennany, E.R.O. (1998). A study on the capability of oxidative stress by hydrogen peroxide to induce atheromatous lesions experimentally in chickens. Ph.D.
Thesis; College of Veterinary Medicine, University of Mosul.
20 . Al-Omer, Z.S.N. (1999). A study of possible biochemical marker of oxidative stress in myocardial infarction: Susceptibility of erythrocytes and plasma to in vitro
challenge with H2O2. Msc. Thesis, College of Pharmacy. University of Baghdad.
21 . Loven, D.P. and Oberley, L.W. (1985). Free radicals, insulin action and diabetes. In: Superoxide Dismutase. III. Disease status. Oberley, L.W.(Ed). Boca Raton.
PP: 151-190.
22 . Kakkar, R.; Kolra, J.; Mantha, S.V. and Parad, K. (1995). Lipid peroxidation and activity of antioxidant enzymes in diabetic rats. Mol Cell Biochem., 151: 113-119.
23. Schreck, R.; Albermann, K. and Baeuerle, P.A. (1992). Nuclear factor KB: an oxidative stress-responsive transcription factor of eukaryotic cell (a review). Free
Radic Res Commun., 17: 221-237.
24. Hennig, B.; Toborek, M.; McClain, C.J. and Diana, J.N. (1996). Nutritional implications in vascular endothelial cell metabolism. J Am College of Nutr.,
15(4): 345-358.
25 . Khudiar, K.K. (2001). The effect of the aqueous extract of parsley (Petroselinum sativum) seeds on lipid metabolism of cholesterol-fed rats. (unpublished data).
26 . Taniguchi, M.; Yasutake, A. and Takedomi, K. (2000). Effects of dietary sulfur-containing amino acids on oxidative damage in rat liver caused by N-nitrosodimethylamine administration. British J Nutr., 84: 211-217.
27 · van Acker, S.A.; van den Berg, D.J.; Tromp, M.N.; Griffioen, D.H. and Bast, A. (1996). Structural aspects of antioxidant activity of falvonoids. Free Radic Biol Med., 20: 331 342.
28. Hempel, J.; Pforte, H.; Raab, B.; Engst, W.; Bohm, H. and Jacobasch, G. (1999). Flavonols and flavones of parsley cell suspension culture change the antioxidative capacity of plasma in rats. Nahrung, 43(3): 201-4. (Midline Abstract).
29. Kreeman, V.; Skottova, N.; Walterova, D.; Ulrichora, J. and Simanek, V. (1998). Silymarin inhibits the development of hypercholesterolemia in rats. Planta diet induced Medica., 64: 138-142.