Effect Of The Aqueous Extract Of Nigella Sativa Seeds On Blood Serum Glucose, Cholesterol and Total Protein In · Alloxan-Induced Diabetic Rabbits
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Published:
Jun 28, 2003
Keywords:
Nigella Sativa, Aqueous Extract, Alloxan-, Blood, Glucose, Cholesterol, Rabbits
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Abstract
The effect of five weeks oral administration of the aqueous extract of Nigella Sativa Seeds (320 mg/kg body weight) on body weight, blood glucose, cholesterol and total protein (albumin and globulin) concentrations in alloxan induced diabetic rabbits was studied.
Twenty-four mature male rabbits were randomly assigned into three groups of eight each. Diabetes was induced experimentally by intravenous injection of alloxan monohydrate (100 mg/kg body weight) into two groups; diabetic group (D), diabetic group which was orally administrated aqueous extract of Nigella Sativa seeds (DN) while the third group served as control(C).
The results showed that the aqueous extract of Nigella Sativa seeds significantly reduced (P<0.01) the blood glucose and cholesterol concentrations in group (DN) and significantly increased the concentration of total protein (albumin and globulin), also inhibited the body weight reduction as compared with diabetic group (D) and the control (C). These results revealed the capability of the extract of Nigella Sativa seeds to ameliorate the biochemical changes induced by alloxan in diabetic rabbits.
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Effect Of The Aqueous Extract Of Nigella Sativa Seeds On Blood Serum Glucose, Cholesterol and Total Protein In · Alloxan-Induced Diabetic Rabbits. (2003). The Iraqi Journal of Veterinary Medicine, 27(1), 27-40. https://doi.org/10.30539/ijvm.v27i1.1094
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How to Cite
Effect Of The Aqueous Extract Of Nigella Sativa Seeds On Blood Serum Glucose, Cholesterol and Total Protein In · Alloxan-Induced Diabetic Rabbits. (2003). The Iraqi Journal of Veterinary Medicine, 27(1), 27-40. https://doi.org/10.30539/ijvm.v27i1.1094
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19. Al-Gahy, A.M. (1999). Amino acid composition and biological effects of supplementing broad bean and corn proteins with Nigella Sativa (black Cumin) cake protein . Nahrung. Oct; 42(5): 290-4.
20. El-Faham and Sawsan, Y. (1994). Comparative studies on chemical compostion of Nigella Sativa seeds and Its Cake (Defa Tedmeal). J. Agric. Sci. 19(7). 2283-2289.
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22. Ardaw, M. S.; Nasrat, H. A. and Bahhssy, A.A. (1994). Serum immunoglobulins Concentrations in diabetic patients. Diabetes Med. 11(4): 384-387.
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24. Liener, E. I. (1980). A Textbook of Constituents of plant Food Stuffs, 2nd ed . Academic press. Asubsidiary of Horcourt Brace lovanvich publishers, New York, London, Toronto, Sundeny, San Francisco.
2. Yamaraji, T.; Kinoshita, M. (1993). Permeability to albumin in diabetic rat. Mgocradium. Circ. Res. 72(5): 947-957.
3. Al-Zubaidi, Z. N.; Babon, H.A. and Flayeh, F.K. (1996). A Theraputic Index of Iraqi Medicinal Plants . P :43. A, AB Printing Co., Baghdad, Iraq.
4. Shah, M. U. (1992). Antidiabetic screening of a folkoric medicational plant prescntation in normal and diabetic rabbits. Faisalabad, Pakistan, UAF: 85.
5. Ayoub, R.S. (1999). Effect of high dose of the aqueous extract of Nigella Sativa seeds on blood glucose level and leucocyte count in rats . Iraqi J. . Vet. Med. Vol. 12, No. (1) pp. 61-64.
6. Harborne, J. B. (1984). Textbook of Phytochemical Methods . A guide to a moderin techniques of plant analysis 2nd Edition , London, NewYork. Chapman and Hall. P. 196-197.
7. Al-Mashhadany, Huda A.J. (1999). The effect of Eucalyptus Camaldulensis leaves extract on serum glucose and protein in induced diabetic rabbits expermintaly. M. Sc. Thesis. College of Vet. Med. Univ. of Baghdad.
8. Barham, D. and Trinder, P. (1972). Analyst, 97-142(Kit).
9. Richmond, W. (1873). Enzyme preparation for cholestrol investigation. Clin.Chem. 19: 1350-1356.
10. Henry, R.J.; Cannon, D.C.; Winkelman, J.W. (1974). Clinical Chemistry, Principles and Techinques, Harper and Row , 2nd ed.
11.Douma, B.T.; watson. W.A; Biggs, H.G.(1971). Albumin standards and the meansurment of serum albumin with bromcresol green.Clin. Chem. Acta , 31:87.
12. Duncon, D.B. (1955). Multiple range and multiple F. test. Biometrics, 11:1- 42.
13. Statistical Analysis System (1992). Sas Users guide statistics (Version seed). Inst. Inc. Cary. Nc., U.S.A.
14. Ganong (1977). Review of Medical physiology, 18th Ed. Medical publication, California.
15. Edward, C.R.W.; Baird, J. D.; Frier, B.M.;Shepherd, J. and Toft, A. D. (1995). Endocrine and metabolic diseases, including diabetes mellitus in : principles and practice of Medicine . Edwards, C.R.W.; Bouchier, I. A.D., Hasiest, C. and Chilvers, E. (eds). 17th ed churchill, Livingstone, Edinburg.
16. Al-Awadi, F.; Fatania,.; Shamte, U. (1991). The effect of a plants mixture extract on liver glucongensis in streptozotocin induced diabetic rats. Diabetes-Res. Dec; 18(4):163-8.
17. Goldstein, T.L. and Brown, M.S. (1977). The LDL pathway in human fibroblaste: a receptor-mediated mechanism for the regulation of cholestrol metabolism. Ann. Rew. Biochem. 46: 897- 930.
18. Pain, M. V. and Garlick, J. P. (1974). Effect of Sreptozotocin diabetes and insulin treatment on the rate of protein synthesis tissue of the rat in vivo. J. of Biol. Chem. 249(14): 4510-4514.
19. Al-Gahy, A.M. (1999). Amino acid composition and biological effects of supplementing broad bean and corn proteins with Nigella Sativa (black Cumin) cake protein . Nahrung. Oct; 42(5): 290-4.
20. El-Faham and Sawsan, Y. (1994). Comparative studies on chemical compostion of Nigella Sativa seeds and Its Cake (Defa Tedmeal). J. Agric. Sci. 19(7). 2283-2289.
21. Kimhall, S.R.; Horestky, R. L. and Jefferson. L. SW. (1995). Hormonal regulation of albumin gene expression in primary cultures of rat heputocytes. Am. J. physiol. 268:6-11.
21. Catalano, C.; Muscell, E.; Quinones, G.A.; Baldi, S.; Masoni, A. Gibb; L. Torffvit. O.; Seyhieri, G. and Ferrannini, E. (1997). Effect of insulin on systemic and renal handling of albumi in diabetic and Niddm Subjects. Diabetes 46 (5):868-75.
22. Ardaw, M. S.; Nasrat, H. A. and Bahhssy, A.A. (1994). Serum immunoglobulins Concentrations in diabetic patients. Diabetes Med. 11(4): 384-387.
23. Tennekoon, K. H.; Jevathayaparan, S.; Kurukulasooriya, Ap; Karunanyake, EH. (1991). Possible hepatoxicity of Nigella Sativa seeds and Dregea Volubills levaves. J. Ethnophamacoi Mar; 31(3): 283-9.
24. Liener, E. I. (1980). A Textbook of Constituents of plant Food Stuffs, 2nd ed . Academic press. Asubsidiary of Horcourt Brace lovanvich publishers, New York, London, Toronto, Sundeny, San Francisco.