Preparation of Listerin antigen as a tool for Diagnosis of listeriosis
Article Sidebar
Published:
Jun 28, 2004
Keywords:
Listeria monocytogenes, listeriosis, guinea pigs
Main Article Content
Abstract
This study was conducted to produce a specific antigen for detection L. monocytogenes infection. The antigen was prepared by culturing L. monocytogenes on suitable media and then disrupted by ultrasonicatorwaves.
The water – soluble extract of sonically disrupted Listeria were used for skin testing guinea pigs infected with (1*108 CFU / ml) of a L. monocytogenes. Two infected doses were used for immunization at intervals of (10) days between them.
Two skin tests were done, 10 days and 15 days after the 2nd infected dose. The skin test results were read after 24 & 48 hrs. which showed clear thickening differences and redness at the injection sites after 24 hrs. and become more clear after 48 hrs.
Downloads
Download data is not yet available.
Article Details
How to Cite
Preparation of Listerin antigen as a tool for Diagnosis of listeriosis. (2004). The Iraqi Journal of Veterinary Medicine, 28(1), 43-54. https://doi.org/10.30539/ijvm.v28i1.1065
Section
Articles
How to Cite
Preparation of Listerin antigen as a tool for Diagnosis of listeriosis. (2004). The Iraqi Journal of Veterinary Medicine, 28(1), 43-54. https://doi.org/10.30539/ijvm.v28i1.1065
References
1- Rouquette, C. & Berch, P. (1996): The pathogenesis of infection by Listeria monocytogenes. Microbiologia. 12. 245-258.
2- Schlech, W.F. (1996): pathogenesis & immunology of Listeria monocytogenes. Pathol. Biol. Paris. 44. 775-782.
3- Miles, A.A; Misra, S. S; and Irwin. J. O. (1983): The estimation of bactericidal power of blood. J. Hyg. 38: 732-749.
4- Hudson, L. & Hay, F.C. practical Immunology, 2nd edition (1980). Blackwell scientific publications. Pp. 328-340.
5- Halliburton, B. L. & Blazkovec, A. A. (1975): Delayed hyper sensitivity and acquired cellular resistance in guinea pigs infected with Listeria monocytogenes. Infect. Immun. 11. 1-7.
6- Mcgregor, D. D & chen- woan, M. (1984): The cell response to Listeria monocytogenes is mediated by heterogenous population of immuno specific T-cell. Clin. Inves. Med. 7. 243.
7- North, R. J. (1975): Nature of memory in T-cell mediated anti bacterial immunity: anamenstic production of mediator T-cell. Infect. Immun. 12. 754-760.
8- Mielk, M; Niedobitek, G.; stein. H; & Hahn, H. (1989): Acquired resistance to Listeria monocytogenes is mediated by lyt - 2* T-cellindependently of the influx of monocytes into granulomatous lesions. J. Exp. Med. 170. 589-594.
9- Harty, J. T. & Bevan, M. J. (1999): Responses of CD3 T-cells to intracellular bacteria. Curr- Opin. Immunol, 11.89-93.
10- Cohen. J. J; Gilberto, E; Rodriguez, P. D. K; & Campbell. P. A. (1975): listeria cell wall fraction AB cell mitogen. J, Immunol. 114. 1132-34
11- Campbell, P. A;sehuffler, C; & Rodriguez, G. C. (1976): Listeria cell wall fraction; AB cell adjuvant. J. Immunol. 116. 590-594.
12- Roots, E. & Strauch, D. (1958): Listeriolisin. P.18. Paul parey in Berlin and Hamburg.
13- Fiedler, F; Seger, J. & Ruhl, G. J. (1984): The biochemistry of murine & cell wall teichoic acids in the genus Listeria monocytogenes. Syst. App. Microbiol. 5. 360-376.
14- Afanso, L. C; Scharton, T. M; Vicira, Q. L.; Wysocka, M.; Trinchieri, G.; & Scott, P. (1994): The Adjuvent effect of inter leukin- 12 in avaccine against leishmania major. Science. 263. 235 - 236.
15- Cleveland, M. G.; Gorhan. J. D.; Murph. T. L.; Tuomanen, E. T.; & Murphy,K. M. (1996): Lipotechoic & Preparation of gram -- Positive bacteria induce interlukin - 12 through a CD14 - dependent pathway. Infect. Immun. 64. 1906.
16- Dai, J. W.; Bartens, W.; Kohler. G.; Hufnagel, M.; Kopf & Brombacher, F. (1997): Impaired macrophages Listericidal and cytotoxin activities are responsible for the rapid death of Listeria monocytogenes infecied IFN - receptor deficient mice. J. Immunol. 158. 5297.
17- SeeLiger,H. P. R. & Hohn, K. (1979): Serotype of Listeria monocytogenes and related species. Methods in Microbiology. Vol. 13, eds Bergan, T.; Norris, J. R. Academic Press - London. PP. 31 - 49. cited by peel, M. ; Donachie , W. and shaw , W. (1988b) : Physical & antigenic heterogeneity in the flagellin of Listeria monocytogenes and ivanovii. J. Gen. Microbial. 134. 2593 -98.
18- Sheehan, B. C.; Kcoks, S.; Dramsi, S.; Gouin, E.; Klarsfeld. A. D.; Menguao, J.; Cossard, P. (1994): Molecular and genetic determinant of the Listeria monocytogenes infection process. Curr. Top. Microbiol. Immunol. 192. 187-16.
19- Schwarzkopf, A. (1996): Listeria monocytogenes: aspects of pathogenicity. Pathol. Biol. Paris. 44. 769-774.
20- Berche, P.; Gaillard, J. L.; Geoffroy, C. J.; Alouf, J.E. (1987a); T cell recognition of listeriolysin is induced during infection Listeria monocytogenes. J. Immunol. 139.3813-3821.
21- Baetz, A. L.; Wesley, I. V.; stevens, M. G. (1996): The use of listeriolysin O in an Elisa, askin test & alymphocyte blastogenesis assay on sheep experimentally infected with Listeria monocytogenes, Listeria ivanovii or Listeria innocua. vet. Microbial. 51. 151-159.
22- Barbuddhe, S. B.; Malik, S.V. S; shomer, B; Gupta, L. K. (1999): Cytotoxic T-cell, delayed type hyper sensitivity and listeriolysin O in experimental bovin listeriosis. Vet. Microbial. 64. 333-341.
2- Schlech, W.F. (1996): pathogenesis & immunology of Listeria monocytogenes. Pathol. Biol. Paris. 44. 775-782.
3- Miles, A.A; Misra, S. S; and Irwin. J. O. (1983): The estimation of bactericidal power of blood. J. Hyg. 38: 732-749.
4- Hudson, L. & Hay, F.C. practical Immunology, 2nd edition (1980). Blackwell scientific publications. Pp. 328-340.
5- Halliburton, B. L. & Blazkovec, A. A. (1975): Delayed hyper sensitivity and acquired cellular resistance in guinea pigs infected with Listeria monocytogenes. Infect. Immun. 11. 1-7.
6- Mcgregor, D. D & chen- woan, M. (1984): The cell response to Listeria monocytogenes is mediated by heterogenous population of immuno specific T-cell. Clin. Inves. Med. 7. 243.
7- North, R. J. (1975): Nature of memory in T-cell mediated anti bacterial immunity: anamenstic production of mediator T-cell. Infect. Immun. 12. 754-760.
8- Mielk, M; Niedobitek, G.; stein. H; & Hahn, H. (1989): Acquired resistance to Listeria monocytogenes is mediated by lyt - 2* T-cellindependently of the influx of monocytes into granulomatous lesions. J. Exp. Med. 170. 589-594.
9- Harty, J. T. & Bevan, M. J. (1999): Responses of CD3 T-cells to intracellular bacteria. Curr- Opin. Immunol, 11.89-93.
10- Cohen. J. J; Gilberto, E; Rodriguez, P. D. K; & Campbell. P. A. (1975): listeria cell wall fraction AB cell mitogen. J, Immunol. 114. 1132-34
11- Campbell, P. A;sehuffler, C; & Rodriguez, G. C. (1976): Listeria cell wall fraction; AB cell adjuvant. J. Immunol. 116. 590-594.
12- Roots, E. & Strauch, D. (1958): Listeriolisin. P.18. Paul parey in Berlin and Hamburg.
13- Fiedler, F; Seger, J. & Ruhl, G. J. (1984): The biochemistry of murine & cell wall teichoic acids in the genus Listeria monocytogenes. Syst. App. Microbiol. 5. 360-376.
14- Afanso, L. C; Scharton, T. M; Vicira, Q. L.; Wysocka, M.; Trinchieri, G.; & Scott, P. (1994): The Adjuvent effect of inter leukin- 12 in avaccine against leishmania major. Science. 263. 235 - 236.
15- Cleveland, M. G.; Gorhan. J. D.; Murph. T. L.; Tuomanen, E. T.; & Murphy,K. M. (1996): Lipotechoic & Preparation of gram -- Positive bacteria induce interlukin - 12 through a CD14 - dependent pathway. Infect. Immun. 64. 1906.
16- Dai, J. W.; Bartens, W.; Kohler. G.; Hufnagel, M.; Kopf & Brombacher, F. (1997): Impaired macrophages Listericidal and cytotoxin activities are responsible for the rapid death of Listeria monocytogenes infecied IFN - receptor deficient mice. J. Immunol. 158. 5297.
17- SeeLiger,H. P. R. & Hohn, K. (1979): Serotype of Listeria monocytogenes and related species. Methods in Microbiology. Vol. 13, eds Bergan, T.; Norris, J. R. Academic Press - London. PP. 31 - 49. cited by peel, M. ; Donachie , W. and shaw , W. (1988b) : Physical & antigenic heterogeneity in the flagellin of Listeria monocytogenes and ivanovii. J. Gen. Microbial. 134. 2593 -98.
18- Sheehan, B. C.; Kcoks, S.; Dramsi, S.; Gouin, E.; Klarsfeld. A. D.; Menguao, J.; Cossard, P. (1994): Molecular and genetic determinant of the Listeria monocytogenes infection process. Curr. Top. Microbiol. Immunol. 192. 187-16.
19- Schwarzkopf, A. (1996): Listeria monocytogenes: aspects of pathogenicity. Pathol. Biol. Paris. 44. 769-774.
20- Berche, P.; Gaillard, J. L.; Geoffroy, C. J.; Alouf, J.E. (1987a); T cell recognition of listeriolysin is induced during infection Listeria monocytogenes. J. Immunol. 139.3813-3821.
21- Baetz, A. L.; Wesley, I. V.; stevens, M. G. (1996): The use of listeriolysin O in an Elisa, askin test & alymphocyte blastogenesis assay on sheep experimentally infected with Listeria monocytogenes, Listeria ivanovii or Listeria innocua. vet. Microbial. 51. 151-159.
22- Barbuddhe, S. B.; Malik, S.V. S; shomer, B; Gupta, L. K. (1999): Cytotoxic T-cell, delayed type hyper sensitivity and listeriolysin O in experimental bovin listeriosis. Vet. Microbial. 64. 333-341.