Association of TGF-β2 Gene Polymorphism with Growth Rate in Local Chickens

  • Ali M. Sahib College of Veterinary Medicine, University of Kufa, Iraq
  • Abbas F Al-Khalisy College of Veterinary Medicine, Baghdad University
  • Mushtaq T Abdulwahid College of Veterinary Medicine, Baghdad University
Keywords: chicken, TGF-β2 gene, single nucleotide polymorphism, productive performance

Abstract

Iraqi native chickens have tasty meat and eggs; however, they are characterized by low production efficiency. In fact, phenotypic traits, such as growth rate, are influenced by genes and environmental factors. During health and disease, a variety of cellular processes such as proliferation, differentiation, motility, adhesion, migration, apoptosis, and immune response regulate the TGF-β genes. The enhancement in body weight can be reached through mass selection, whereas feed conversion ratio (FCR) is relatively more difficult to improve. This means, selecting for body weight has been submitted as an effective way of indirectly improving feed conversion ratio. Therefore, the present study attempts to identify associations between productive traits and polymorphism of TGF-β2 gene in local Iraqi chicken. Seventy-five male birds were used in this study. The restriction enzyme RsaI has been used to detect the target region (284 bp) in the TGF-β2 gene. A single nucleotide polymorphism (SNP) was identified at the position 62 in the exon 1 region of TGF-β2 by using PCR-RFLP and DNA sequencing technique. The genotypic frequencies were 46.7, 40, and 13.3% for CC and TC and TT genotypes, respectively. While the allele frequency of C and T were 0.67 and 0.33%, respectively. Generally, during the last period of rearing the best significant (P<0.05) improve in the body weight, weight gain and FCR were recorded in the TT genotype of the TGF-β2 gene. In conclusion, a functional sequence in the genome could be attributed to the mutation. Therefore, genotype of the TGF-β2 gene could be exploited to select the best individual as a parent to the next generations for improving of growth rate in

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References

Yacoub HA, Fathi MM. Phylogenetic analysis using d-loop marker of mtDNA of Saudi native chicken strains. Mitochond. DNA. 2013; 24: 538.551.

Yacoub HA, Fathi MM, Sadek MA. Using cytochrome b gene of mtDNA as a DNA barcoding marker in chicken strains, Mitochond. DNA. 2015; 26: 217.223.

Fathi MIC, Motawei MI, Abou-Emera OK, El-Zarei MF. Evaluation of genetic diversity of Saudi native chicken populations using microsatellite markers. Poult Sci. 2017; 96(3): 530.536.

FAO. Local chicken genetic resources and production systems in Indonesia. Prepared by Muladno. GCP/RAS/228/GER Working Paper. 2008; No. 6. Rome.

Zuidhof MJ, Schneider BL, Carney VL, Korver DR, Robinson FE. Growth, efficiency, and yield of commercial broilers from 1957, 1978, and 2005. Poult Sci. 2014; 93:2970.2982.

Abdulwahid MT. Effect of injection hatching eggs with Newcastle disease vaccine and different doses of vitamin E on some productive traits and immune response of broilers. Iraqi J. Vet. Med. 2015; 39(2): 98-107.

Scanes CG, Harvey S, Marsh JA, King DB. Hormones and growth in poultry. Poult Sci. 1984; 63: 2062-2074.

Deeb N, Lamont SJ. Genetic architecture of growth and body composition in unique chicken population. Journal Hered. 2002; 93: 107-118.

Naqvi AN. Applications of molecular genetic technologies in livestock production: Potentials for developing countries. Adv. Biol. Res. 2007; 1: 72.84.

Du X, Chen C, Yuan Z, Zhang L, Chen X, Wang Y, et al. Genetic polymorphisms of Mc4R and IGF2 gene association with feed conversion efficiency traits in beef cattle. Pak. Vet. J. 2013; 33(4): 418-422.

Li H, Deeb N, Zhou H, Mitchell A, Ashwell C, Lamont SJ. Chicken quantitative trait loci for growth and body composition associated with transforming growth factor-ƒÀ genes. Poult. Sci. 2003; 82: 347-356.

Enayati B, Rahimi-Mianji G. Genomic growth hormone, growth hormone receptor and transforming growth factor b-3 gene

polymorphism in breeder hens of Mazandaran native fowls. Afr. J. Biotechnol. 2009; 8(14): 3154-3159.

Abasht B, Dekkers JC, Lamont SJ. Review of quantitative trait loci identified in the chicken. Poult Sci. 2006; 85: 2079.2096.

Wang SZ, Hu XX, Wang ZP, Li XC, Wang QG, Wang YX, et al. Quantitative trait loci associated with body weight and abdominal fat traits on chicken chromosomes 3, 5 and 7. Genet. Mol. Res. 2012; 11: 956.965.

Lorda-Diez CI, Montero JA, Garcia-Porrero JA, Hurle JM. TGF-ƒÀ2 and 3 are coexpressed with their extracellular regulator Ltbp1in the early limb bud and modulate mesodermal outgrowth and BMP signaling in chicken embryos. BMC Dev. Biol. 2010; 10(1): 69.

Lu Y, Chen S, Yang N. Expression and methylation of FGF2, TGF-B and their downstream mediators during different developmental stages of leg muscles in chicken. PLoS ONE. 2013; 8(11): e79495.

Jin S, Chen S, Li H, Lu Y, Zhang D, Ji C, et al. Polymorphisms in the transforming growth factor ƒÀ3 gene and their associations with feed efficiency in chickens. Poult Sci. 2013; 92(7): 1745.1749.

Gu L, Sun C, Gong Y, Yu M, Li S. Novel copy number variation of the TGFƒÀ3 gene is associated with TGFƒÀ3 gene expression and duration of fertility traits in hens. PLoS ONE. 2017; 12(3): e0173696.

Lu SX, Wu CX. Research and application of animal genetic marker-assisted selection. Yi Chuan. 2002; 24:359-362.

National research council (NRC). Nutrient requirements of poultry. 9th ed. National academy press. Washington. D.C. USA; 1994.

Malek M, Lamont SJ. Association of INOS, TRAIL, TGF-ƒÀ2, TGF-ƒÀ3, and IgL genes with response to Salmonella enteritidis in poultry. Genet. Sel. Evol. 2003; 35(Suppl 1): S99-S111.

Tohidi R, Idris I, Panandam JM, Bejo MH. The effects of polymorphisms in IL-2, IFN-ƒÁ, TGF-ƒÀ2, IgL, TLR-4, MD-2, and iNOS genes on resistance to Salmonella Enteritidis in indigenous chickens. Avian Pathol. 2012; 41(6): 605-612.

Muhsinin M, Ulupi N, Gunawan A, Wibawan IWT, Sumantri C. g.640T>C Polymorphism of the TGF-ƒÀ2 gene is associated with Salmonella pullorum resistance in Indonesian chicken. Animal Prod. 2017; 19(2): 81-92.

Al-zubaidie SSA. Poultry management. 1st edition. College of Agriculture. Basra University; 1986.

Cary N. Statistical analysis system, User's guide. Statistical. Version 9. SAS. Inst. Inc. USA; 2012.

Edwards AWF. GH Hardy (1908) and hardy.weinberg equilibrium. Genetics J. 2008;179(3): 1143-1150.

Keambou TC, Hako BA, Ommeh S, Bembide C, Ngono EP, Manjeli Y, et al. Genetic diversity of the Cameroon indigenous chicken ecotypes. Int. J. Poult. Sci. 2014; 13(5): 279.

Dayton WR, White ME. Cellular and molecular regulation of muscle growth and development in meat animals. J. Anim. Sci. 2008; 86: 217-225.

Wu MY, Hill CS. TGF-ƒÀ superfamily signaling in embryonic development and homeostasis. Dev. Cell. 2009; 16: 329-343.

El-Tahawy WS, Abdel-Rahman MM. Molecular, sequencing and bioinformatics of insulin-like growth factor 1(IGF-1) gene and transforming growth factor ƒÀ2 gene associations with growth traits in three strains of chicken. Preprints J. 2020; 1-22.

Carlson CM, Turpin EA, Moser LA, O'Brien KB, Cline TD, Jones JC, et al. Transforming growth factor-ƒÀ: activation by neuraminidase and role in highly pathogenic H5N1 influenza pathogenesis. PLoS Pathogens. 2010; 6(10): e1001136.

Zhang P. Mechanisms and regulation of transforming growth factor superfamily mediated gene expression [dissertation]. Michigan, USA: University of Michigan; 2012.

Cooley JR, Yatskievych TA, Antin PB. Embryonic expression of the transforming growth factor beta ligand and receptor genes in chicken. Dev. Dyn. 2014; 243: 497-508.

Javelaud D, Mauviel A. Mammalian transforming growth factor-betas: Smad signaling and physio-pathological roles. I. J. B. C. B. 2007; 36 (7): 1161-6115.

Poniatowski .A, Wojdasiewicz P, Gasik R, Szukiewicz D. Transforming growth factor beta family: insight into the role of

growth factors in regulation of fracture healing biology and potential clinical applications. Mediat. Inflamm. 2015; 1-17.

Teixeira AF, Dijke PT, Zhu H. On-target anti-TGF-ƒÀ therapies are not succeeding in clinical cancer treatments: what are remaining challenges. Front. Cell Dev. Biol. 2020; 8(605): 1-18.

Massague J, Cheifetz S, Endo T, Nadal-Ginard B. Type beta transforming growth factor is an inhibitor of myogenic differentiation. Proc. Natl. Acad. Sci., USA. 1986; 83: 8206-8210.

Roberts AB, Sporn MB. The transforming growth factor-ƒÀs. In: Peptide growth factors and their receptors I. Springer, New York, NY; 1991. P. 419-472.

Burt DW, Law AS. Evolution of the transforming growth factor-beta superfamily. Progr. Growth Factor Res. 1994; 5:99-118.

Roark EF, Greer, K. Transforming growth factor-beta and bone morphogenetic protein-2 act by distinct mechanisms to promote chick limb cartilage differentiation in vitro. Dev. Dyn. 1994; 200: 103-116 .

Wall NA, Hogan BL. TGF-beta related genes in development. Curr. Opin. Genet. Dev. 1994; 4: 517-522.

Jakowlew SB, Ciment G, Tuan RS, Sporn MB, Roberts AB. Pattern of expression of transforming growth factor-beta 4 mRNA and protein in the developing chicken embryo. Dev. Dyn. 1992; 195: 276-289 .

Jakowlew SB, Ciment G, Tuan RS, Sporn MB, Roberts AB. Expression of transforming growth factor-beta2 and beta 3 mRNAs and proteins in the developing chicken embryo. Differention J. 1994; 55: 105-118.

Sanders EJ, Wride MA. Roles for growth and differentiation factors in avian embryonic development. Poult Sci. 1997; 76: 111-117 .

Saxena VK, Sachdev AK, Gopal R, Pramod AB. Roles of important candidate genes on broiler meat quality. World's Poult. Sci J. 2009; 65: 37-50.

Loveridge N, Farquharson C, Hesketh JE, Jakowlew SB, Whitehead CC, Thorp BH. The control of chondrocyte differentiation during endochondral bone growth in vivo: Changes in TGF-beta and the proto-oncogene c-myc. J. Cell Sci. 1993; 105: 949-956.

Jakowlew SB, Dillard PJ, Winokur TS, Flankers KC, Sporn MB, Roberts AB. Expression of transforming growth factor-beta s 1-4 in chicken embryo chondrocytes and myocytes. Dev. Biol. 1991; 143: 135-148 .

Joyce ME, Jingushi S, Bolander ME. Transforming growth factor-ƒÀ in the regulation of fracture repair. Orthop Clin North Am. 1990; 21(1): 199-209.

Tsiridis E, Upadhyay N, Giannoudis P. Molecular aspects of fracture healing: which are the important molecules. Injury J. 2007; 38(1): S11-S25.

Xu X, Zheng L, Yuan Q, Zhen G, Crane JL, Zhou X, et al. Transforming growth factor-ƒÀ in stem cells and tissue homeostasis. Bone Res. 2018; 6: 1-31.

Bennett AK, Hester PY, Spurlock DM. Relationships of transforming growth factor .ƒÀ2 single nucleotide polymorphism and messenger ribonucleic acid abundance with bone and production traits in chickens. Poult Sci. 2007; 86: 829-834.

Tang S, Ou J, Sun D, Zhang Y, Xu G, Zhang Y. A novel 62-bp indel mutation in the promoter region of transforming growth factor-beta 2 (TGFB2) gene is associated with body weight in chickens. Anim. Genet. 2011; 42(1): 108-112.

Chen S, An J, Lian L, Qu L, Zheng J, Xu G, et al. Polymorphisms in AKT3, FIGF, PRKAG3, and TGF-ƒÀ genes are associated with myofiber characteristics in chickens. Poult Sci. 2013; 92: 325-330.

Moreira GCM, Poleti MD, Pertille F, Boschiero C, Cesar ASM, Godoy TF, et alUnraveling genomic associations with feed efficiency and body weight traits in chickens through an integrative approach. BMC genetics. 2019; 20(1): 1-14.

Al-Khalisy AFS. The effect of using cod liver oil to broilers diet on some production and physiological traits. Al-Anbar J Vet. Sci. 2011; 4(2): 193-200 .

Published
2021-06-27
How to Cite
Sahib, A., Al-Khalisy, A., & Abdulwahid, M. (2021). Association of TGF-β2 Gene Polymorphism with Growth Rate in Local Chickens. The Iraqi Journal of Veterinary Medicine, 45(1), 9-16. https://doi.org/10.30539/ijvm.v45i1.1034