Stereological and Histopathological Effects of Trigonella foenum-graecum Seeds on Rabbits Ovary
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Abstract
This study aimed to investigate the impacts of the Trigonella foenum-graecum (T. foenum-graecum) seeds on the female gonad. A total of twenty local rabbits were used in this study; were divided into four groups (5 each): first group (G1) was considered as the control group. The second group (G2), third group (G3) and fourth group (G4) were fed daily1.5%, 3%, and 4.5% of T. foenum-graecum seeds respectively for 60 days (twice daily). At the end of the experiment, the animals were euthanized by diethyl ether (C2H52O). Then the abdomen was incised, and the samples of ovaries were collected and fixed by 10% neutral buffered formalin. The histological assessment was done with a paraffin embedding technique and the histological sections were stained with Hematoxylin and Eosin stain. The result showed that the numbers of primary and secondary follicles were significantly (P<0.05) decreased in G3and G4 compared with the control (G1) and G2. The numbers of Graafian follicles were significantly P<0.05 decreased G4 compared with other groups. The diameters of the primary, secondary, and Graafian follicles were significantly smaller than the other groups. The thickness of the granulosa cell layer in G3and G4 were significantly thinner than the other groups. The histological figures declared that the ovary of G2 was similar to that in G1. The histological sections of G3 and G4 were revealed marked cortical and medullary vascular congestion and focal hemorrhage; there were also marked follicular degeneration and cystic necrosis. The study concluded that the low concentration of T. foenum-graecum (fenugreek) seeds do not have any positive effect in terms of ovarian stimulation.
Correspondence: [email protected]
Received: 23 March 2020
Accepted: 1 September 2020
Published: 28 December 2020
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Allaw AK, Belqees AK, Yousof Z, Al-Ani I, Bracamonte M, Sadeeq A. Is there any effect of fenugreek seeds aqueous extract (FSA) on the quantity of ovarian follicles and estrus cycle of female rats. JMPR. 2016; 1(1): 55-63.
Atila UG, Uslu H, AdaliY. Hepatoprotective and nephroprotective effects of Trigonella foenum-graecum L. (Fenugreek) seed extract against sodium nitrite toxicity in rats. BMRAT.2019:6 (5): 3142-3150.
Al-Yasery AJ, Khaleel IM, Al-Aameli MH. Histological and hemato-biochemical study of fenugreek (Trigonella foenum) and ginger (Zingiber officinale) aqueous extracts on ovary structure in white swiss mice (Musmusculus). LSA.20173; (2): 962-973.
Cahill LP, Mariana JC, Mauleon P. Total follicular populations in ewes of high and low ovulation rates. RRF. 1979;55 (1): 27-36.
Danko J. Changes in ovarian mass, size and number of follicles in post parturientewes. ActaVeterinaria Brno. 1997;66 (2): 71-4.
Dande PA, Patil SU. Evaluation of aponins from Trigonella foenum graecum seeds for its ant fertility activity. Asian J Pharm Clin Res. 2012;5 (3):154-7.
Dawood TN, Al-Deri AH, Kareem EH. Effect of Trigonella foenum graecum seeds on the productive, reproductive performance and some biochemical traits in the local rabbits. IJSR. 2015; 6(5): 904-908.
Dorostghoal M, Mahabadi MK, Adham S. Effects of maternal caffeine consumption on ovarian follicle development in wistar rats offspring. JRI. 2011;12 (1): 15-22.
Bancroft JD, Gamble M. Theory and practice of histological techniques.6th. USA: Elsevier health sciences; 2008; 168-173 p.
Suad KA, Al-Shamire JS, Dhyaa AA. Histological and biochemical evaluation of supplementing broiler diet with β-hydroxy-methyl butyrate calcium (β-HMB-Ca). IJVR. 2018; 19(1): 27.
SAS. Statistical Analysis System, User's Guide. Statistical. 2010; V 9.1th ed. SAS. Inst. Inc. Cary. N.C. USA.
Solomon T, Largesse Z, Mekbeb A, Eyasu M, Asfaw D. Effect of Rumexsteudelii methanolic root extract on ovarian folliculogenesis and uterine histology in female albino rats. AJOL. 2010;10(4): 353-361.
Esson S, Ric L, Ekin O, Krush I. Effect of fenugreek (Trigonella foenum) on Gonad development and body vital values in mice. IJCH.2005; 64 (4): 387-395.
Khaleel AA, Hamad KR. Effects of fenugreek (Trigonella foenum-graecum) seeds aqueous extract on the tissue structure of anterior pituitary gland and some organs of reproductive system in male albino rats. JOPAS. 2011; 3(3): 102-115.
Prakash AO, Saxena V, Chand GK, Mathur R. Antifertility investigation on embelinan oral-contraceptive of plant-origin. Effect on Uterine Biochemical-Constituents of Ovariectomized Albino-Rats. Comparative. JOPAE. 1983; 8(4): 271-275.
Jhon M, Cumbuger H, Gandwinterhoff H. Oxidation product of caffaic acid mode of substance for the antigonadotrophic activity of plant extract. Planta Medica. 1989; 6: 14-17.
Nikolić Z, Mrvić V, Ivanov I, Blagojević Z. Effect of neonatal estrogen and HCG treatment on the genital organs of adult female rats. ActaVeterinaria Hungarica. 1999; 47(1): 109-15.
Lee CJ, Park H, HandKim JK. Natural and radiation induced degeneration of primordial and primary follicles in mouse ovary. ARS. 2003; 59(2): 109-117.
Kaisser A, Gabari A, Habeb M, Majid M. Evaluation of the potential activity effect of fenugreek (Trigonella foenum) in female rabbits. IJBS. 2006; 7(3): 31-42.