Evaluation of transfer factor protective efficacy against Tuberculosis in Guinea Pigs Ekram,A.A. AL-Samarrae , Khalifa A. Khalifa , Noria ,R.S. Mohammed

Abstract

Transfer factor has been prepared from sensitized guinea pigs against heat
killed Mycobacterium bovis antigen and protective efficacy were evaluated in
guinea pigs that gave 1 ml of transfer factor equivalent 5x108 cell / ml of sensitized
and normal cell donor; then challenged with dose 0.01 mg / animal of virulent
Mycobacterium bovis, After 40 days of challenge all animals were sacrificied.
Histologically: - TFs recipient group showed a mono-nuclear cells
infiltration’s (Lymphocytes & Macrophages) with a mild emphysema of lungs, and
liver showed mononuclear cells ,a small necrotic foci in the lymphocytic organs
(spleen & lymph nodes); the kidney showed mononuclear cells in glomeruli and
urinary tubules ;While the TFn recipient group were an areas of caseated necrotic
materials surrounded by macrophages &Lymphocytes, congestion of internal
organs (Liver and Spleen) and Sever congestion with hemorrhage of alveoli tissue,
sever emphysema & consolidation in lung kidney showed tuberculle foci in
glomeruli and around urinary tubules .
There were no pathgenomic changes in heart muscles of TFs recipient group while
a mild congestion in TFn recipient group.