Cytopathogenicity studied of fowl pox virus by using indirect immunoperoxidase and acridine orange tests . Harith M. AL-Hyali , Aied B. AL-Zughaibi and Suad Abdulkarim.

Abstract

Locally isolated fowl pox virus was inoculated on chorioallantoic membrane
of chick embryos, leads to the appearances of odema and pocks lesion on the
membrane , 120 hours post inoculation .Virus assay showed that the infectivity
titer were 106.8 to 107.5 EID50/0.1 ml in embryonated chicken eggs. Monolayer
tissue cultures of chick embryo fibroblast cells infected with fowl pox virus were
examined by acridine orange staining and indirect immunoperoxidase test to study
the cytopathogenic effects of the virus . The most striking cytoplasmic changes
observed was the presence of the intracytoplasmic inclusions at 24-48 hours post
inoculation, numerous inclusions were clearly seen at 72 hr. P.I.in addition to
cytoplasmic vaculation and granulation were clearly seen at 96 hr. P.I. These
changes stained brilliant green with acridine orange and dark brown staining with
immunoperoxidase . Both tests demonstrated the localization of pox virus antigens
in infected cells at same intervals.