Effect of Supplimenting Black Seeds (Nigella Sativa Linn) in the Ration on the Immune Response of Newcastle Disease and Some Physiological Aspects of Broiler Chicken Atheer Kassab , Aamer Rassam

Abstract

The study was conducted to investigate the role of supplementing the diet of
broiler chicken with Black seed (Nigella sativa Linn) broiler chicken in the
immune response of the bird .The study has also investigated the level of some
serum proteins and the activity of amino group transmitting enzymes. The Black
seeds have been ground and supplemented in the diet of the chickens at 2%
inclusion rate .A total of 80 Faobro broiler chicks were divided equally into 4
groups.
Group 1: given Black seed supplemented diet and vaccinated at 8 ,16 and 26 days
of age with one dose of live Newcastle (ND) vaccine in the crop and injected 0.2
ml oil adjuvant ND vaccine subcutaneously.
Group 2: given Black seed supplemented diet and vaccinated at 8 ,16 and 26 days
of age with one dose of live Newcastle (ND) vaccine in the crop but did not
injected with oil adjuvant ND vaccine.
Group 3: A control to group 1, but not supplemented with Black seed in the diet.
Group 4: A control to group 2, but not supplemented with Black seed in the diet.
Results showed that the supplementation of Black seed with the diet caused
significant increase in the total serum protein, the serum globuline, the ND
antibody examined by Haemagglutination test and the body weight. The
differences were more obvious when the birds were vaccinated with oil adjuvant
vaccine .The enzyme Aspartate aminotransferase (AST) and Alanine
aminotransferase(ALT) have not been affected by the inclusion of 2% Black seed
in the diet. It was concluded that the inclusion of 2% Black seed in the diet has
led to a better immune response and health conditions shown by the birds.