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Genetically altered stable non reverting aromatic dependent (aro)
Salmonella typhimurium,strain SL1479 was administrated intramuscularly to
healthy pregnant guinea pigs as alive vaccine. Twenty one pregnant guinea pigs
were divided into two groups, the first group (15 animals) was vaccinated twice
with 1ml containing 17 C.f.U /ml approximately fourth and second week preparturition
and the second group (6 animals) injected with 1ml trypticase soy
broth (TSB) as a control group.
Adverse reaction to vaccination were not observed in the pregnant guinea
pigs, which parturated normally. The vaccine induced humoral and cellular
immune response as measured by tube agglutination test and delayed type
hypersensitivity(DTH)-skin test in the immunized dams and transfer of this
response to the newborns, which revealed a high titers of
O(somatic)&H(flagller) agglutination titers and positive delayed type
hypersensitivity(DTH)- skin test.
The newborn overcome the challenge with virulent Salmonella
typhimurium at 3,6 &8 weeks of age, compared with the control
newborn which died. These results revealed the efficacy of the
prenatal vaccination with aro Salmonella typhimurium to transfer the
passive immunity to the newborn.