The Use Of Alternative Stain In Rose Bengal Test Antigen Preparation Which Specific For Brucellosis. Erada A .J . Saeed , Sahar A. Al_Bayatti

Abstract

The Rose Bengal test is one of the famous diagnostic test of Brucellosis
specially as a screening test in order to detect the infection in limited area.
Rose Bengal standard stain which producd by specific companies was used in
preparation of the test special antigen , The stain gives the known pink colour
for the antigen during the test that make the agglutination in positive cases more
visible due to the reaction between antigen and the specific antibodies of
Brucella which found in the serum sample of human and different animals.
Antigen for the Rose Bengal test in this study is prepared by using alternative
stain easily found in local supermarkets using for food colours and not
expensive like standard stain. All standard tests were down for the stain like the
colour ,pH, stability are same for two stains until the date of expire of antigen.
The antigen prepared with alternative stain was used in comparative with the
antigen prepared with standard stain for testing serum samples of human and
different animals, the results deal no significant different statistically between
them that means as a result we can prepare antigen more easily and not
expensive.