EVALUATION OF ANTIMICROBIAL ACTIVITY OF WATERY AND ALCOHOLIC EXTRACTS FOR ANCHUSA STRIGOSA ON GROWTH OF GRAM POSITIVE PATHOGENIC BACTERIA ISOLATED FROM PHARYNGITIS AND TONSILLITS CASES HANAN A. AL-AYMI , AMINA N. AL-THAWANI, FARID J. AL-TAHAN

Abstract

The effects of Anchusa strigosa plant extract were studied in respect to
their of gram positive bacterial growth inhibition. were isolated from cases of
pharyngitis and tonsillitis . alcoholic and hot water extracts of the plants as well
as their dried powders were prepared . The preliminary chemical tests revealed
acidic pH of all extracts.
The dried powder , watery and alcoholic extracts of A. strigosa
contained resins ,tannins , phenols, flavonoids, glycosides and avery little
amount of alkaloids in its watery and alcoholic extract.The alcoholic extract of
A. strigosa showed more patent inhibitory effect on resistant bacteria than its
watery extract and the best effect was on growth of Strept.salivarius and
Strept.pyogenes inhibition zone diameter 27.0,26.0 mm. In the present study ,
the Minimum inhibitory concentration (MIC) and Minimum bacteriocidal
concentration (MBC) of the plants extracts were measured for the more
predominant gram positive isolates and the results varied according to different
kinds of plant extracts and different types of bacteria . The least values of MIC
and MBC were for alcoholic extract of A. strigosa on Staph.epidermidis which
valued 10% ,20% respectively .It has been noticed that the watery extracts of A.
strigosa , have a sedative effect when given dosing 5 g/kg for laboratory
mice , these extracts however, showed a synergistic sedative effect when mixed
with pentobarbitone and caused prolongation of sleeping time in experimental
mice .Neither the alcoholic nor the watery extracts of the plants showed any
toxic effect on the laboratory mice after oral dosing of 2.5 – 15 g /Kg B.W.
Watery and alcoholic extracts, Anchusa strigosa , Pharyngitis ,Tonsillitis .
Pathogenic bacteria