The Expermintal Infection of L.monocytogenes &Isolation it from Egg, Oviduct & Ovary

Abstract

The aim of the present study to detect the transmission of L.monocytogenes
from experimental infected chickens to Egg products ,Ovary,Oviduct
.&histopathological changes in these organs.
2groups of chickn ,1st group(12 animals)infected with L.monocytogenes
1ml/chick.SC,(1×1010 cfu/ml),2nd group injected 1ml/chick normal salin as
control.eggs were collected for isolate Listeria ,animal killed in
(7,15,24,30,34,39,42)days,for isolateListeria from ovary,oviduct&samples
taken for histopathological examination.
The results show theres no characterstic signs in infected chickens except
depression,anoxia&and theres marked decrease of egg production,
qualititativly & quantitativly.
L.monocytogenes isolated from egg ( shell, ,yolk,albumine)&persiste to
(31,28&19) dayses respectively,also Listeria isolate from ovary&oviduct in
chickens which killed in (7,15,24,30)day,&not isolate from chickens that
killed in day (39,34,42)or in control.
The histopathological changes theres congestion of the organs ,diffuse
infiltration of infilammatory cells such as lymphocyte , neutrophils &
macrophage. also theres odeama, multi necrosis foci &desqumation.