Treatment and prevention of Salomellosis in puppies using Lactobacillus acidophilus Al-Samarrae, S.A.G.; Al-Judi, A.M.H.; Muhsen. R.K.

Abstract

This study was carried out to evaluate the ability of Lactobacillus
acidophilus to prevent and treat Salmonella typhimurium in puppies. In vitro
antibiotic sensitivity test of S. typhimurium was made and the result revealed
that Ciprofloxacin is the most effective.Isolation of L. acidophilus from the
intestinal tract of the normal dogs and also revealed that all Lactobacillus strains
were biochemically identical to standard strain. A bacterial strain that had high
ability to inhibit the growth of S. typhimurium in vitro with high ability to
adhere to intestinal epithelial cells and tolerate the low pH and bile salts was
chosen for the experimental study.
Twenty puppies were divided into four groups and treated in different ways.
The clinical, haematological and biochemical parameters were obtained from all
animals at the period of two days before inoculation until the death of animals of
the first group at the sixth day post infection. The results revealed that , puppies
experimentally infected with S.typhimurium showed both septicemic and
gastrointestinal forms of the disease accompanied with isolation of
S.typhimurium from the blood and stool throughout the experiment. The
statistical analysis of the results of all parameters among all groups revealed ,for
the first time, that L.acidophilus plays an important role in the prevention of
S.typhimurium infection in puppies . It also has high therapeutic effect against
S.typhimurium, which was almost similar to that of Ciprofloxacin.