Study of Using Thymbra spicata leaves to reduce the toxic immunosuppresive effect of aflatoxin in broilers Hatem M. M. Al-Naemey, Nwal S. Ja`afar and Hassiba A. Omran

Abstract

The present study performed to evaluate the impact of addition of
Thymbra spicata leaves powder of aflatoxin contaminated ration in
reduction of the aflatoxin immuno suppressive effect in the immune
response induced by Newcastle disease vaccine in broilers.
The study conducted in three groups, The first group fed aflatoxin free
ration, second group fed aflatoxin contaminated ration without any
treatment, while the third group fed aflatoxin contaminated ration
supplemented of 2% Thymbra spicata leaves powder.
All chick groups were vaccinated twice with Newcastle disease vaccine
(NDV) at 10 and 20 days of age. ELIZA test was carried out to estimate the
humeral immune response at 6, 19 and 31 days of age. Infection challeng
were done at day 32 of age with velogenic ND strain (Z-2003).
The results showed that the first group has high significante antibodies titer
reach to 3239±249.3 at 19days of age and 10186+845.3 at 31 days of age
with significant level 0.01 and high protection ratio against challenge
(100%)at 32 days of age, while the second group has the low antibodies titer
reach to 2622.6±27.3 at 19 days of age and 5712.5±786.9at 31daysof age
and low protection ratio against challenge(66.67), the third group’s
antibodies titer and protection ratio lower than first group and higher than
second group ,the titers are 3060±542.5 at 19 days of age and 5712.5±786.9
at31 days of age while the protection ratio against challenge is 80% at 32
days of age .
The results showed the immuno suppressive effect of aflatoxin as in second
group and the role of Thymbra spicata leaves powder in reduction of these
effect.