Fluorescent Antibody Technique For Detection Of Salmonella Typhimurium In The Intestine Of Experimentally Infected Calves Faisal, G. Habasha and Abdul Wahab Abul Razak

Abstract

A systemic study on the pathogenesis of experimentally induced
Salmonella typhimurium infection was carried out in calves. Sixteen normal,
colostrum-fed, friesian calves, ranging in age from 3 to 6 weeks were used. The
calves were divided into two equal groups. Group I calves were inoculated
orally with 1.5 x 1011 CFU of Salmonella typhimurium and group II calves
served as control.
Using an indirect fluorescent antibody technique, it was found that
Salmonella typhimurium has an apparent predilection for the small intestine
particularly jejunum and ileum. The results of this study suggest that this
technique can be considered as a rapid reliable technique for diagnosis of
salmonellosis in endemic outbreaks.