Comparative study between the prophylactics Effects of aqueous extract of Black Currant (Vitis vinferia.L) and vitamin E on some biological parameters related with heart diseases in oxidative Stressed rats Abdul-Katum, A. H. and Khudair, K.K.

Abstract

This study was designed to investigate the prophylactic effect of aqueous
extract of black currant concentrate on some biological markers related with
heart disease in male rat treated H2O2. It also aimed at comparing the
prophylactic effect of black currant concentrate to that of vit E.
Forty adult male rats were divided randomly into equal groups (ten
rat/group) & were treated as follows for 42 days. Rats in the first group (G І)
were received normal water with oral intubation of sun flower oil 1ml /rat and
consider as control group. Animals of the second group (G Π) were received
0.5% H2O2 in drinking water, while rats of the third group (G Ш) were received
0.5% H2O2 in drinking water with oral intubation of vit E 400 I.U/Kg.B.W
diluting in sun flower oil for each rat daily .While animals in the fourth group (G
ІV) were intubated daily 60mg/kg body weight of aqueous extract of black
currant concentrate plus 0.5% H2O2 in drinking water.
Fasting blood samples were collected at 0, 21, and 42 days of experiment
to study the following parameters:
A - Platelet count (PC) and prothrombin time (PT).
B- Serum concentration of total cholesterol TC, triacylglycerol TAG, High
density lipoprotein-cholesterol HDL-C, Low density lipoprotein-cholesterol
LDL-C and Very low density lipoprotein- cholesterol VLDL-C of each group
were measured. These parameters were regarded as biomarkers of
atherosclerosis and coronary heart disease (CHD). Furthermore section of heart
& aorta were assessed for histopathogical studies.
The result revealed that administration of 0.5% H2O2 in drinking water for six
weeks (42days) caused significant increase (p<0.05) in platelet count & in serum
TC,TAG,LDL-C, and VLDL-C concentration with significant decrease(p<0.05)
in prothrombin time and HDL-C concentration as compared to other groups, on
other hand oral intubation of vitamin E or aqueous extract of black currant
concentrate in addition to H2O2 (groups Ш and ІV respectively) decreased the
serum concentration of TC, TAG, LDL-C, VLDL-C, and platelet count
Iraqi Journal of Veterinary Medicine Vol. 32, No. 2, 2008
228