Study influence of Salinomycin and Anticoccidial Vaccine on Pathological changes in intestine of Broiler Chickens experimental infected with Eimeria spp.

Abstract

To conduct the influence of salinomycin & anticoccidial vaccine on
pathological changes in intestine of broiler chickens experimental
infected with Eimeria spp. By using 40 broiler chicks divided randomly
to four groups( 10 chicks of each group ) First group vaccinated with
anticoccidial vaccine (coccivac) at 8 days of age in drinking water while
the second group feeded salinomycin in concentration 60ppm from one
day old ,the third group left non vaccinated nor given salinomycin as a
control group at(26 days )of age chicks in that three groups infected with
different species of Eimeria spp .with high dose (744x74³) Oocyst /chick
by group inoculation & the fourth group consider negative control not
vaccinated or treated .At (33 days)of chicks age all the chicks were killed
for measuring lesion score of intestine which their results +1.75 , +3, +4
with mortality rate 0%,10%,20% for vaccinated ,salinomycine treated &
control groups respectively then took samples for Histopathological
changes the results pointed high pathological changes in intestine of
positive control group with sever necrosis & degeneration of epithelia
with sever tissue damage companied with maturation first & second
generation sporozoite in the endothelial layer of intestine. While the
pathological changes was less sever in salinomycine treated group with
necrosis & degeneration of epithelial layers accompanied with exist of
sporozoite in endothelial layer of intestine . while the vaccinated group
showed no clear pathological changes in intestine except hyperplasia of
global cells & infiltration of lymphocytes cells in the layers of intestine ,
it was concluded that the vaccine which was given to chicks provide good
protection & decrease dangerous of coccidiosis infection .