Control of the infection with Brucella melitensis in cattle Hussein.A.S , Abd-Alrahman.A.M , Nadia A.A

Abstract

Sera from1722 cattle of different age (3 months to 8 years) were
examined by the Rose Bengal and tube agglutination test: 134 cow give
positive result and the overal Brucella seroprevalence was (8%) milk
samples zeropositive milking cattle were cultured on Brucella selecative
media.
Seropositive cattle (102) were treated for the first time with long acting
oxytetracyclin at the dose of 20 mg/kg of body weight administered
intramuscularly (i.m) every 2 days for 30 days and streptomycin at 25
mg/kg (i.m) every 2 days for 16 days.
The regimen was found to be effective in eliminating the shedding of
Brucella organisms by cattle in milk.
Moreover all treated cattle became zeronegative within 16 months after
treatment…zeronegative cattle (1588)were vaccinated for the first time
with the Br.Melitensis as follows
1. 362 young cattle(aged three months to one year)were each inoculated
subcutaneously with a full dose(1×106)viable organisms in 1 ml, Brucella
antibody titers were detected 2-4 weeks post vaccination then decreased
gradually until the animals became zeronegative 8 months after
vaccination.
2. 1226cattle aged more than one year were each inoculated
subcutaneously with reduced dose (1×103 viable organism in 1
ml)antibody titers measured 2-4 weeks post vaccination then decreased
gradually until the animals became zeronegative 3 months post
vaccination.No Brucella organisms were seen from repeated udeer
secretion samples from all vaccinated milking cattle, and no abortions
were recorded among pregnant vaccinated cattle.