Effect of of Levamisole as immunostimulatoron the humoral and cellular immune response in sheep vaccinated with Brucella melitensis Rev-1 vaccine Yousif, A, A ; Talal A.K andAl- saigh,M,N

Abstract

This study was designed to determined the influence of levamisole
administration as immunostimulator for sheep vaccinated with Brucella
melitensis vaccine (Rev-1). The sixty sheep (males and females) were
divided equally into four groups *The first group (vaccinated group ) the
animals were vaccinated with Rev-1 vaccine at a dose of 1 ml s/c which
contain 2 x 109 viable bacteria *The second group ( vaccine and
levamisole ), was vaccinated with Rev-1 as the same of the first group as
well as levamisole was given orally at a dose of 10 mg / kg B.W a week
before vaccination and with vaccination and a week after vaccination and
then levamisole was given at monthly intervals up to the end of the
experiment.* Third group (levamisole group) was given levamisole orally
at a dose of 10 mg / kg B.W at the same regimen of the time of the
second group.
*The fourth group kept as a control group which was given 1 ml of PBS
s/c
The humeral and cellular immunity assessed on animals at 0 time
,2,4,6,8,10,12,14 weeks.The vaccinated animals showed a high
temperature after vaccination in the Ist and 2nd groups, the highest rate
value was recorded in the first group 40.93C. Same trends were noticed in
respiratory and pulse rates. No changes were recorded in the third and
fourth groups.
The vaccine stimulate humeral immunity ( first and second groups ).
Rose Bengal Test gave a positive result in both vaccinated groups only.
The second group revealed high means significant antibody titer 1160.53
than the first group, while the third and fourth groups showed a negative
result in the antibody titer as detected by passive haemagglutination test
(PHA).
While the cellular immunity showed the following results .Delayed type
hypersensitivity test was positive in the vaccinated groups and the second
groupshowed significantly higher average in redness area and its
thickness, than the first group,while in third and fourth groups the results
were negative .