Studying the efficacy of giving anthrax toxin as a vaccine intranasally and subcutaneously

Abstract

This study was concerned on the production of Bacillus anthracis
toxin and its use as a vaccine. Two groups of guinea pigs were
immunized with the toxin which was given in a dose of 0.5 ml
subcutaneously to the 1st group while the animals of the 2nd group were
immunized by instillation in the nose 4 drops into each naris. The two
groups were given booster dose 14 days after the 1st dose. The immunized
and control animals were bled by cardiac puncture 20 days after the
booster dose.
In the present study, anthraxin was used as an antigen in Enzyme
Linked Immuno Sorbant Assay (ELISA) to measure the optical density
(OD) obtained from adminsteration of vaccine in two different routes.
High mean of optical density 1.660 ± 0.07 nanometer was recorded to
subcutaneous group and the mean 1.181 ± 0.002 nanometer to the
intranasal group.