The effect of Aloe Vera leaf gel in promoting wound healing and as antibacterial Ali Aziz Al-Khayyat , Lubna Ahmed Kafi and Zena Munther A. Fahmi

Abstract

This study was carried out to explore the effect of Aloe Vera leaf
gel in promoting wound healing and to investigate the
antibacterial effect against some pathogenic bacteria in comparison
with Nigella sativa oil. Standard dilutions of Aloe Vera leaf gel were
made from ten to one hundred percent and its antibacterial effect
had been examined in seeded agar method against Staphylococcus
aureus and Pseudomonas aeruginosa both were previously identified
by laboratory and biochemical methods, Nigella Sativa oil which is
known to be one of the important medicinal plant was used for
comparison . Statistical analysis showed a significant difference
(P<0.05) between AVG and Nigella Sativa oil.
The effect of Aloe Vera leaf gel against Staph .aureus was more
potent than against Pseudomonas aeruginosa. There was a
proportional relation between different concentrations of AVG
with the values of inhibition zones diameters of the bacteria.
Results showed that Aloe Vera was more potent than Nigella sativa
against both bacteria.
The Minimum Inhibitory Concentration (MIC) was sixty
mg/ml and the Minimum Bactericidal Concentration (MBC) was
eighty mg/ml for Staph .aureus.
In order to investigate the effect of Aloe Vera gel on wound
healing, twenty-four local male rabbits were used. They were
divided into four equal groups and each animal was wounded in
both sides of the back region by making a one by two centimeter
square standard longitudinal incision with surgical scalpel. The
first group was a control group (wounded without treatment); this
group was employed to observe the normal wound healing. The
second group was treated with crude Aloe Vera leaf gel twice daily
for 10 days. While the third group was wounded and infected with
the pathogenic bacteria Staphylococcus vaureus without treatment,
in order to observe the natural body defense against pathogenic
microorganisms. The last group was infected with the same
bacteria but treated with crude Aloe Vera leaf gel to observe the
antibacterial effect. The wounds in the left side in second and
fourth group were left as self-control.