study the effect of alcoholic and watery extracts of garlic and onion plants on growth of candida albicans and Cryptococcus neoformans in vitro&vivo

Abstract

This research included study the effect of garlic and onion plants
extracts(alcoholic and watery) in vitro in three different concentrations
15%,25%,35% and in vivo in experimental white mice .Research was
performed by three experiments, first one was conducted to study
effectiveness of different concentration of alcoholic and watery garlic
extract on growth of candida albicans and Cryptococcus neoformans in
vitro, showed that the effect of alcoholic extract on the growth of candida
albicans was inhibitory,started from 0.4 mm to 0.1 mm compared with
control plats 4.2 mm ,where as the results of the effect on the growth of
Cryptococcus neoformans showed more clearness and the inhibition
started from 0.6 to inhibit all the growth in plat in comparison with
control plats1.4 mm. While the effect of watery garlic extract showed less
effect and the inhibition began from 0.5 mm to 0.2 mm for candida
albicans , but the growth inhibition of Cryptococcus neoformans began
from 0.4 to 0.15 mm.The second experiment was the same as the first
experiment , but using alcoholic and extracts onion , the growth of
candida albicans inhibited by alcoholic exract from 0.6 mm to no growth
in the plat , but the inhibition of Cryptococcus neoformans was started
from 0.5mm to 0.2 mm for alcoholic onion extract. While the watery
onion extract effect on the growth of candida albicans the inhibition
started from 1.6 mm to 1 mm ,but the inhibition of Cryptococcus
neoformans was began from 1 mm to 0.3 mm.
Third experiment was study the effect of crude garlic and onion alcoholic
extract ointment 1% on experimental infection in mice , using 30
experimental mice divided to 6 equal groups,each group include 5 mice
*groups which infected with candida albicans treated :
The group 1,2,3,expermrutly infected with candida albicans ,where as
group 3,4,6 were infected with Cryptococcus neoformans for 1,2,3 group
,treted with the ointment of alcoholic extract of garlic, group 2 treated
with alcoholic extract ointment of onion, where as group 3 left with no
treatment as a control group