Immune relationahip between Pseudomonas aeruginosa and Listeria monocytogenes Mohammed Jawed Alwan ,Khalid yassin zagir Nagham ,mohammed Al-Jebouri

Abstract

In order to known the effect of whole sonicated L. monocytogenes antigen on as mice experimentally
infected with P.aeruginosa.,30 white mice,8-12 weeks age, were divided randomly into three equal
groups..The 1st group was immunized subcutaneously twice with (0.5)ml of whole sonicated P.aeruginosa
antigen(7.6mg/ml concentration protein) ,with two weeks intervals.The cellular immune responses was
checked at (27) days post-immunization The animals of the 1st and 2nd groups were challenged
subcutaneously with (0.5) ml of bacterial suspension contain1X10 9cfu/ml of virulent P.aeruginosa while
the 3rd group was inoculated S/C with (0.5)ml of sterile phosphate buffer saline and served as control
negative group.. The resuls showed that the prepared antigen antigen induced a good
protection in the immunized animals which was,characterized by survival all immunized animals and no
bacterial isolates and pathological changes in the internal organs after infection with P.aeruginosa as
compared with nonimmunized infected animals,which died after infection, with severe bacterial isolates
and sever acute suppurative reaction in their internal organs