Determination of Patulin By TLC From Mouldy Apples Omar J. Al saree ;Khalid N., Jassim Thkra A. Hassen

Abstract

Analysis of the decayed apples (van. Starking delicious), collected from the local
market, for patulin mycotoxin, using column chromotography and TLC Plates showed
that the presence of the amount of patulin mycotoxin in the mouldy tissues (1.3 , 2.5 ,
3.4 , 4.2, 5.5, and 6.4 ) cm in diameters were (5.1, 5.8, 6.3, 10.5, 12.3, and 14 ) μg
patulin/g moldy tissues respectively. According to this research also, Patulin
mycotoxin could diffused to the surrounding mouldy tissues. It is found that (0.5and
0.2), (1.0 and 0.5), and (1.5 and 0.5) μg patulin/g tissues in the first and second
centimeters after mouldy tissues 4.2, 5.5 and 6.4 cm respectively, this phenomenon
due to the presence of intracellular spaces in the tissues which allow to patulin to
diffuse from mouldy to the healthy tissues there was no patulin found in the other
surrounding healthy tissues. Gram positive bacteria found to be more susceptible to
patulin, the zone of inhibition against Staphyllococcus aureus was 10 to 11 mm in
diameter at concentration 10 and 30 mg /ml respectively when compared with 0.3
μg/ml streptomycin and 0.06 μg/ml ciprofloxacin while no inhibitory effect found
against Gram negative bacteria Klebsiella pneumoniae and Escherichia coli