Toxicopathological study on the effect of different toxic doses of Euphorbia granulata F. crude extract in albino mice

Abstract

The purpose of this study was to evaluate the effect of different toxic doses
with regard to toxicopathological changes in mice injected with ethanolic crude
extract of Euphorbia granulata F. The experiment was done on (24) mice of
(6-8weeks age and 20-25g weight ) divided equally into (4) groups as follows:-
First group injected distilled water subcutaneously for 45 days and considered
as control ,second group injected subcutaneously (600 mg/kg B.W/day)
ethanolic extract of E. granulata for 45 days, Third group injected
subcutaneously (800 mg/kg B.W/day) ethanolic extract of E. granulata for 45
days. Fourth group injected subcutaneously (1000 mg/kg B.W/day) ethanolic
extract of E. granulata for 45 days.The microscopic findings of internal target
organs revealed that the severity of the lesion increased with the dose of the
plant extract. Animals injected with (1000 mg/kg B.W) showed fibrosis in liver,
kidney, myocardium with encephalomalacia and aggregation of astrocytes and
microglial cells.Furthermore the study showed mononuclear infiltrations in
some internal organs, with multiple granulomatous reaction and perivascular
lymphocytic cuffings. Amyloid deposition in spleen with nodular hyperplasia of
liver and hyperplasia of epithelial lining of endometrium.In conclusion, toxic
doses of ethanolic crude extract of Euphorbia granulata F cause severe
toxicopathological changes in important organs and tissues in addition the
active compound of plant may act as immune stimulant, with formation of
precancerous lesions in liver and uterus.