Hematological and Neurotoxic Effects of Endosulfan Pesticide on Common Carp Cyprinus carpio
Main Article Content
Abstract
The present study including determined blood picture by measuring red blood cells count, hemoglobin concentration, packed cell volume and white blood cells in common carp Cyprinus carpio, as well as description behavior and growth of carp fish .In order to estimate LC50 used 240 fingerlings of common carp Cyprinus carpio were exposed to 0.0008μg/L, 0.0010μg/L, 0.0011μg/L, 0.0012μg/L, 0.0013μg/L, 0.0014μg/L and 0.0015μg/L. The LC50 of endosulfan was 0.0012μg/L for 48h of exposure Fish behavioral were recorded that showed abnormalities after exposure to the various endosulfan concentrations such as increase swimming activity, hypersensitivity, jerky movement, violent movements, loss of equilibrium, hyperactivity, increase operculum movement, frequent jumping, swimming at the water surface, erratic swimming, spiraling, convulsion, escape attempts from the aquarium with respiratory stress and decrease in respiratory rate as well as a significant decrease at (P<0.05) in body weight of all treated groups. The result of blood picture showed a significant reduction in red blood cells count, hemoglobin concentration, packed cell volume values while the number of white blood cells was showed a significant increase in its values.
Downloads
Article Details
How to Cite
References
Abraham, CC. (2004). Endosulfan’s effects: Omissions and flawed data. Environ. Health Perspect., 112: A538.
Kumari, B.; Madan, VK. and Kathpal, TS, (2007). Pesticide residues in rain water from Hisar, India. Environ Monit Assess 133:467-71.
Evans, MS.; Muir, D.; Lockhart, WL.; Stern, G.; Ryan, M. and Roach, P. (2005). Persistent organic pollutants and metals in the freshwater biota of the Canadian Subarctic and Arctic: an overview. Sci. Total Environ. 351-352:94-147.
Anon, (2002). Hazardous Substances Data Book (HSDB)- US National Library of Media – Canadian Centre for Occupational Health and Safety.
Nowak, BF.; Goodsell, A. and Julli, M. (1995). Residues of endosulfan in carp as an indicator of exposure conditions. Ecotoxicology,
: 363-371.
Salvo, LM.; Sinhorini Maludelli, BE.; Klmez COlmedo, DC.; Sanchez, NL.; Carboni,M I.; Malucelli, BM. and Silva, H. C(2008). Effects of endosulfan sublethal concentrations on carp (Cyprinus carpio, Linnaeus, 1758): morphometric, hystologic, ultrastructural analyses and cholinesterase activity evaluation. Braz. J. Vet. Res. Anim. Sci., 45(2): 87-94.
Lombardi, JV.; Machado-Neto. GJ.; Brossi-Garcia, AL.; Marques, HL. and Kubo, E. (2001). Acute toxicity of the pesticides endosulfan and ametryne to the freshwater prawn Macrobrachium rosenbergii De man. Bull Environ Contam.Toxicol., 67: 665-671.
Wirth, EF.; Lund, SA.; Fulton, and Scott, GI. (2002). Reproductive alterations in adult grass shrimp (Palaemonetes pugio), following sublethal, chronic endosulfan exposure. Aquat. Toxicol., 59: 93-99.
Rao, JV.; Begum, G.; Pallela, G.; Usman, PK. and Rao, RN.(2005).Changes in behavior and brain acetylcholinesterase activity in mosquito fish Gambusia affinis in elation to sublethal exposure of chlorpyrifos. Intl J Environ Res Public Health 2(3-4): 478-483.
Rajasekaran, G.; Kavitha, M.; Sudha, R. and Kannan, K. (2009). Impact of temperature on behaviour and respiratory responses of catfish Clarias gariepinus. J. Ecobiol., 24(3): 263-268.
Bradbury, S P. and Coast, J R.(1989).Toxicokinetics and toxicodynamics of pyrethroid insecticides in fish.Environ Toxicol Chem.,
: 373-380.
Pandey, AC. (1988). Impact of endosulfan (Thiodan) EC 35 on behavior and dynamics of oocyte development in the teleostean fish, Colisa (Trichogaster) fasciatus. Ecotoxico. and Env. Safety, 15: 221-225.
Sunderam, RI.; Cheng, DM. and Thompson, GB. (1992). Toxicity of endosulfan to native and introduced fish in australia. Environ. Toxico. Chem., 11: 1469-1476.17. ASTM (2008). Standard guide for measurement of behavior during fish toxicity
tests.E1711–95. Am Soc Test Mater, 12.
Jensen, EG.; Skaare, JU.; Egaas, E. and Goksoyr, A. (1991). Response of xenobiotic metabolizing enzymes in rainbow trout (Oncorhynchus mykiss) to endosulfan, detected by enzyme activities and immunochemical methods. Aquatic Toxicology,
: 81-91.
Olsen, RE.; Sundell, K.; Mayhew,TM.; Myklebust, R. and Ring, E.(2006). Acute stress alters intestinal function of rainbow trout, Oncorhynchus mykiss (Walbaum). Aquaculture, 250(1-2): 480- 495.
Barbieri, E.; Serralheiro, P C. and Rocha, I O.(2002). The use of metabolism to evaluate the toxicity of dodecil benzen sodium sulfonate (LAS-C12) on the Mugil platanus(mullet) according to the temperature and salinity. J. Exp. Mar Biol. Ecol., 277(2): 109-127.
ASTM (2008). Standard guide for measurement of behavior during fish toxicity tests.E1711–95. Am Soc Test Mater, 12.
Dembele, K.; Haubruge, E. and Gaspar, C. (2000): Concentration effects of selected insecticides on brain acetylcholinesterase in the common carp (Cyprinus carpioL.). Ecotoxicology and Environmental Safety, 45: 49-54.
Weyts, FA.; Cohen, N.; Flik, G. and Kemenade, BM. (1999).Interactions between the immune system and the hypothalamo-pituitaryinterrenal axis in fish. Fish & Shellfish Immunology, 9: 1-20.
United States Environmental Protection Agency (USEPA). (2006). Assessme Lindane and other hexachlorocyclohexane isomers.U.S.Environmental Protection Agency. http://www.epa.gov/fedrgstr/EPA- PEST/2006/February/Day- 08/p1103.htm
Řehulka, J.; Minařik, B. and Řehulkovà, E. (2004).Red blood cell indices of rainbow trout, Oncorhynchus mykiss (Walbaum) in aquaculture. Aqua. Res., 35: 529-546.
Simonato, JD.; Guedes, CL. and Martinez, CB. (2008). Biochemical, physiological and histological changes in the neotropical fish prochilodus lineatus exposed to diesel oil. Ecotoxicol. Environ. Saf., 69: 112-120.
Jenkins, F. ;Smith, J.; Rajanna, B.; Shameem, U.; Umadevi, K.; Sandhya, V. and Madhavi, R. (2003).Effect of sub-lethal concentrations of endosulfan on hematological and serum biochemical parameters in the carp (Cyprinus carpio). BullEnviron Contam Toxicol
: 993-997.
Drastichova, J.; Svobodova, Z.; Luskova, V. and Machova, J. (2004).Effect of cadmium on hematological indices off common carp, Cyprinus carpio L. Bull Environ ContamToxicol 72: 725-732.
Banik, S.; Chakraborty, S. and Choudhury, JR.(1996).Haemolytic anaemia in Anabas testudineus with reference to endosulfan. Uttar Pradesh. J. Zoo, 16: 87-88.
Ogundiran, MA.; Fawole, OO.; Adewoye, SO. and Ayandiran, TA.(2009). Pathologic lesions in the gills of Clarias gariepinus exposed to sublethal concentrations of soap and detergent effluents. J. Cell Anim. Bio., 3 (5): 078-082.
Sweilum, MA. (2006).Effect of sublethal toxicity of some pesticides on growth parameters, haematological properties and total production of Nile tilapia (Oreochromis niloticusL.) and water quality of ponds. Aquaculture Research,
: 1079–1089.
Jee, JH.; Masroor, F. and Kang, JC. (2005). Responses of cypermethrin- induced stress in haematological parameters of Korean rockfish, Sebastes schlegeli(Hilgendorf). Aqua. Res., 36: 898–905.
Joshi, P. and Deep, H. (2002). Effect of lindane and malathion exposure to certain blood parameters in a freshwater teleost fish Clarias batrachus. Poll Res., 21: 55-57.
Matkovics, B. and Witas, HO. (1981).Paraquat as an agent affecting antioxidant enzymes of common carp erythrocytes.Comp. Biochem. Physiol. , 87C: 217-219.
Gill TS and Pant JC (1985). Erythrocytic and lecocytic responses to cadmium poisoning in a freshwater fish, Puntius conchonius Ham. Environ. Res., 36:327-337.
Srivastava, AK. and Mishra, S. (1979). Blood dyscrasia in teleost, Closia fasciatusassociated with cadmium poisoning. J. Comp. Pathol., 89:1-5.
Chandrasekar, S. and Jayabalan, N. (1993). Hematological responses of the common carp Cyprinus carpio L. exposed to the pesticide endosulfan. Asian Fish Soc. 6: 331-340.
John, P.(2007). Alteration of certain blood parameters of freshwater teleost Mystus vittatusafter chronic exposure to Metasystox and Sevin. Fish Physiol. Biochem., 33: 15-20.
Mahajan, CL. and Dheer, JS. (1979). Cell types in the peripheral blood of an airbreathing fish Channa punctatus. J Fish Biol 14: 481-4