Isolation and Identification of Escherichia coli and Salmonella typhimurium from Sheep in Baghdad city الاء طه حنون و اكرام عباس عبود السامرائي
Article Sidebar
Main Article Content
Abstract
One hundred fifty fecal samples of sheep were collected from September, 2017-March, 2018 in Baghdad city areas (Abu-Grab zone, Dora zone, Saydyia zone, Arab jbure zone, Al-Usfia zone, Al-Fudhalia zone and College Veterinary medicine –University of Baghdad). Samples were cultured on MacConkey and Eosine Methylene Blue agar for E.coli isolation, Xylose Lysine Deoxycholate and Salmonella –Shigella agar for S. typhimurium isolation. Results showed high percent (78.57%) of infection in female of E.coli than 68.42% in Salmonella typhimurium. E.coli was recovered with an infection rate 93.33% in Al-Fudhalia area, and S. typhimurium with an infection rate 12.66% in Arab Jbur. Also, the results showed an infection rate of E.coli 78.75% (110/140) and 21.43% (30/140), and S. typhimurium was recovered in 68.42% (13/19) and 31.58% (6/19) in females and males respectively .
Downloads
Article Details
How to Cite
References
Tenaillon ,O.; Skurnik, D.; Picard , B. and Denamur , E.(2010). The population genetics of commensal Escherichia coli. Nat. Rev .Microbial., 8:207- 217.
Anderson, G.G.; Goller, C.C.; Justice, S.; Hultgren, S.J. and Seed, P.C.(2010). Polysacchride capsule and sialic acid –mediated regulation promote biofilm –like intracellular bacterial communities durincystitis . Infect. Immun., 78(3):963-975.
Kaper, J .B .; Nataro , J. P . and Mobley, H.L.T. (2004). Pathogenic Escherichia coli. Nat. Rev. Microbial., 2:123- 140.
Rodostits, O.M.; Gay, O.M.; Hincheliff, K.W. and Coststable, K.W. (2007) . Veterinary Medicine. A text book of the cattle, horses, sheep, pigs and goats. 8th ed. Slsevier. USA:163.
Sandler, R.S.; Stewart, W.F.; Liberman, J.N.; Ricci, J.A. and Zorich, N.L. (2000). Abdominal pain, bloating and diarrhea in the United
States:prevalence and impact. Dig. Dis. Sci., 45(6): 1166-1171.
Schoenian, S. (2007). Diarrhea (scours) in small ruminants. Maryland cooperative Exttension. Sheep and Goats specialist Western Maryland Research and Eduction Center.
Ansaruzzaman, M.; Albert, J.; Nahar, S.; Byun, R.; Katouli, M.; Kuhn,I. and Mollby, R. (2000). Clonal groups of enter pathogenic isolated in case control studies of diarrhea in Bangladesh .J. Med. Microbiol., 49: 177-185.
Bavaro,M.F.(2012). E.coli and other oxigenic strains: The cures of global food distribution. Curr. Gastroenterol. Rep., 14: 317-323.
Gouali, M. and Weill, F.X.(2013). Enterohemorragic Escherichia colitopical Enterobacteriaceae. Presse. Med., 42(1):68-75.
Sonja, J.O.; Miller, G.; Breuer, T.; Kennedy, M.; Higgins, C.; Walford, J.; McKee, G.; Fox, K.; Bibb, W. and Mead, P.(2002). A Water borne
outbreak of Escherichia coli and haemolytic uraemic syndrome implicated for rural water system .Emerg.Infect. Dis.,8(4):370-375.
Mastroeni, P., Maskell, D., 2006, Salmonella infections: clinical, immunological, and molecular aspects. Cambridge University Press,
Cambridge.
Brenner, F.W. Villar, R.G. Angulo, F.J. Tauxe, R. and Swaminathan, B. (2000): Salmonella nomenclature. J. Clin. Microbiol
.,38: 2465-2467.
Wray, C. and Davis, R. (2003): The epidemiology and ecology of Salmonella in meat-producing animals. In: Torrence, M. E. and Isaacson, R. E. (eds): Microbiological Food Safety in Agriculture Current Topics. Iowa State: Black Well Publishing Company. PP :73-82.
Quinn, P.J.; Carter, M.E.; Markey, B. and Cater, G.R. (2004). Clinical Veterinary Microbiology. Elsevier Limited,6th ed.Mos by Wolfe,London. PP: 66-85.
Schulze, J.; Schiemann, M. and Sonnenborn, U. (2006). 120 years of E.coli its importance in research and medicine .6th ed. Alfred-Nissle -Gesellschaft, Germany. PP: 11-13.
Nielsen, E.M.; Scheutz, F.; and Torpdahl, M.(2006). Continuous surveillance of Shiga toxin-producing Escherichia coli infections by pulsedfield gel electrophoresis shows that most infections are sporadic. Foodborne Pathog. Dis.,3:81-87.
Fotadar , U.; Zaveloff, P. and Terracio, L., (2005). Growth of E. coli at elevated temperatures. J. Basic Microbial., (5): 403–404.
Nadhom,B.N.(2016). Extractionof biofilm produced by Escherichia colithat are isolated from animals infected with diarrhea and study its protective role.Kufa. J.Vet. Med. Sci., 7 (1):
Khalil, S. A. and Eraky, Mona, I.(2012). Microbiological study of Escherichia Coli in Sheep. J. Vet. Sci., 37 ( 1 ):62-68.
Osman, K.M.; Mustafa, A.M.; Elhariri, M. and Abdelhamed, G.S. (2012). The distribution of Escherichia coliSerovars, virulence genes, gene association and combinations and virulence genes encoding serotypes in pathogenic E.coli recovered from diarrheic calves, sheep and goats. Transbound Emerg. Dis., 21. doi: 10.1111/j.1865-1682.2012.01319.
Oloya, J.; Theis, M.; Doetkott, D.; Dyer, N.; Gibbs, P. and Khaitsa, M.l.(2005). Evaluation of Salmonellaoccurrence in domestic animals and humans in North Dakota. Foodborne Pathog. Dis., 4(4):551-630.
AL-Karawiy, H. A. (2008). Isolation and identification of Salmonella typhimurium and detection of gene encoded type-1- fimbriae by using polymerase chain reaction. MSc. thesis, Vet. Med. University of Baghdad, Iraq.
Wessam, M. and Saleh, M. (2012). Isolation of Salmonella spp. from slaughtered sheep in Busrah.Bas.J.Vet.,11(2):
Kamil M.; Ali, K. and Bayan, H.(2015). Detection of Salmonella spp in different food source in Baghdad
city .J. Adv. Res. Biol. Sci., : 2348-8069