The Protective Role of Date Palm Pollen (Phoenix dactylifera L.) on Liver Function in Adult Male Rats Treated with Carbon Tetrachloride

Abstract

The present study was carried out to investigate the protective role of date palm pollen
aqueous suspension against the toxic effects of carbon tetrachlorideon liver function in adult
male rats by studying the following parameters,estimation of ALP, AST, ALT enzymes
activity, totalserum bilirubin, and histological study of liver.Forty adult male rats aged 12-14
weeks and weighed 275-325 gm were randomly divided into four equal groups(10 rats/group)
and were treated for 42 days as follows : rats of the first group were received 1 ml tap water
orally once a day and olive oil 0.5 ml /kg B.W. intraperitonelly twice a week which
considered as group C, rats of the second group were received date palm pollen suspension
orally (150 mg/kg B.W) once a day and olive oil 0.5 ml /kg B.W. intraperitonelly twice a
week( group T1),rats of the third group were treated intraperitonelly with 500mg / kg B.W. of
CCL4 mixed with equal volume of olive oil twice a week (group T2) ,while rats of the fourth
group were received date palm pollen suspension (150 mg/kgB.W) once a day orally and
treated intraperitonelly with 500 mg / kg B.W. of CCL4 mixing with equal volume of olive
oil twice a week (group T3). The blood samples were collected at (zero, 14, 28, 42) days for
biochemical parameters. After that, six rats from each group were sacrificed, and then
samples of liver were taken for histological study. The results revealed no significant
differences (P > 0.05)in Liver Enzymes activity (ALT , AST , ALP ) as well asserum
Bilirubin (TSB) inT1 group treated with date palm pollen comporting with control group
while a significant elevation(P ≤ 0.05) in liver Enzymes (ALT , AST , ALP ) activity and total
serum bilirubin (TSB) in group T2 which exposed to carbon tetrachloride.the protective role
of date palm pollen against carbon tetrachloride was recorded in group T3 which manifested
by significant differences (P>0.05) in liver enzymes activity (ALT , AST , ALP ) and serum
bilirubin (TSB) as compared to the control.The histological study of liverof date palm pollen
treated rats (group T1) indicated proliferation of kupffer cells and no nuclear lesion, while
group T2 showed severe vacuolation in the hepatocytes, while group T3 showed moderate
vacuolation in hepatocytes.From the result obtained ,it was concluded that it seems likely that
dosage of rats with (150 mg/kg B.W) of date palm pollen for 42 days of treatment caused
improvement and enhancement of liver function against carbon tetrachloride harmful effects.