Detection of Canine Parvovirus in Baghdad city by PCR technique Ahmed F. Ahmed1 ,Shony M. Odeisho1 ,Zhala A. Karim

Abstract

Canine parvovirus 2 (CPV2) is a highly contagious and fatal disease of dogs, causing
acute hemorrhagic enteritis and myocarditis. In this study different mutant strains of the virus
were characterized by polymerase chain reaction (PCR).The fecal samples from infected dogs
suspected for CPV2 infection were collected in a suitable medium. The viral DNA from fecal
samples was extracted using specific kits, PCR were carried out with five different primer,
pCPV-2ab and pCPV-2b, to distinguish the strain prevalent in field condition. The primer
pCPV-2ab recognized both variant CPV-2a and CPV-2b, whereas the primer pCPV-2b
recognized only the variant CPV-2b, using the third primer pCPV to recognize the residual
base pair, enabling the differentiation of CPV-2a variant from CPV-2b in field isolates. The
different PCR products were further analyzed by using gel electrophoresis.