The Differences in Bioactivity between Crude and Purified Cholera Toxin from Local Isolate of Vibrio cholera Amina, N. Al- Thwani1 and Khlood, A. Al-Khafaji

Abstract

In this investigate Cholera toxin was purified from the local clinical isolate
designated as V. cholerae S . Few steps were employed for purification of CT
including concentration of the protein, back extraction, and gel filtration. The
bioactivity of CT reflected that crude and purified toxin had lethal activity on Balb/c
mice in which a dose of 2.5µg/ ml of purified toxin caused mice death with
symptoms such as muscle cramps and tachycardia while erythematous and
induration was seen at intradermal I. d injection of skin of Guinea pig.
Histopathological examination for liver, intestine, adrenal gland and spleen of white
mice showed that liver tissue was more affected compared with spleen, intestine and
adrenal gland tissues.